BRD7 (NM_013263) Human Untagged Clone

SKU SC115318

BRD7 (untagged)-Human bromodomain containing 7 (BRD7), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: BRD7
• Synonyms: BP75; CELTIX1; NAG4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013263, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGCAAGAAGCACAAGAAGCACAAGTCGGACAAACACCTCTACGAGGAGTATGTAGAGAAGCCCTTGA; AGCTGGTCCTCAAAGTAGGAGGGAACGAAGTCACCGAACTCTCCACGGGCAGCTCGGGGCACGACTCCAG; CCTCTTCGAAGACAAAAACGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGAAAGGAGAG; AAGCAGATTCCAGGGGAAGAAAAGGGGAGAAAACGGAGAAGAGTTAAGGAGGATAAAAAGAAGCGAGATC; GAGACCGGGTGGAGAATGAGGCAGAAAAAGATCTCCAGTGTCACGCCCCTGTGAGATTAGACTTGCCTCC; TGAGAAGCCTCTCACAAGCTCTTTAGCCAAACAAGAAGAAGTAGAACAGACACCCCTTCAAGAAGCTTTG; AATCAACTGATGAGACAATTGCAGAGAAAAGATCCAAGTGCTTTCTTTTCATTTCCTGTGACTGATTTTA; TTGCTCCTGGCTACTCCATGATCATTAAACACCCAATGGATTTTAGTACCATGAAAGAAAAGATCAAGAA; CAATGACTATCAGTCCATAGAAGAACTAAAGGATAACTTCAAACTAATGTGTACTAATGCCATGATTTAC; AATAAACCAGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGAATGAAAATTCTTAGCC; AGGAAAGAATTCAGAGCCTGAAGCAGAGCATAGACTTCATGGCTGACTTGCAGAAAACTCGAAAGCAGAA; AGATGGAACAGACACCTCACAGAGTGGGGAGGACGGAGGCTGCTGGCAGAGAGAGAGAGAGGACTCTGGA; GATGCCGAAGCACACGCCTTCAAGAGTCCCAGCAAAGAAAATAAAAAGAAAGACAAAGATATGCTTGAAG; ATAAGTTTAAAAGCAATAATTTAGAGAGAGAGCAGGAGCAGCTTGACCGCATCGTGAAGGAATCTGGAGG; AAAGCTGACCAGGCGGCTTGTGAACAGTCAGTGCGAATTTGAAAGAAGAAAACCAGATGGAACAACGACG; TTGGGACTTCTCCATCCTGTGGATCCCATTGTAGGAGAGCCAGGCTACTGCCCTGTGAGACTGGGAATGA; CAACTGGAAGACTTCAGTCTGGAGTGAATACTTTGCAGGGGTTCAAAGAGGATAAAAGGAACAAAGTCAC; TCCAGTGTTATATTTGAATTATGGGCCCTACAGTTCTTATGCACCGCATTATGACTCCACATTTGCAAAT; ATCAGCAAGGATGATTCTGATTTAATCTATTCAACCTATGGGGAAGACTCTGATCTTCCAAGTGATTTCA; GCATCCATGAGTTTTTGGCCACGTGCCAAGATTATCCGTATGTCATGGCAGATAGTTTACTGGATGTTTT; AACAAAAGGAGGGCATTCCAGGACCCTACAAGAGATGGAGATGTCATTGCCTGAAGATGAAGGCCATACT; AGGACACTTGACACAGCAAAAGAAATGGAGATTACAGAAGTAGAGCCACCAGGGCGTTTGGACTCCAGTA; CTCAAGACAGGCTCATAGCGCTGAAAGCAGTAACAAATTTTGGCGTTCCAGTTGAAGTTTTTGACTCTGA; AGAAGCTGAAATATTCCAGAAGAAACTTGATGAGACCACCAGATTGCTCAGGGAACTCCAGGAAGCCCAG; AATGAACGTTTGAGCACCAGACCCCCTCCGAACATGATCTGTCTCTTGGGTCCCTCATACAGAGAAATGC; ATCTTGCTGAACAAGTGACCAATAATCTTAAAGAACTTGCACAGCAAGTAACTCCAGGTGATATCGTAAG; CACGTATGGAGTTCGAAAAGCAATGGGGATTTCCATTCCTTCCCCCGTCATGGAAAACAACTTTGTGGAT; TTGACAGAAGACACTGAAGAACCTAAAAAGACGGATGTTGCTGAGTGTGGACCTGGTGGAAGTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013263 unedited; TTCAACAATTAGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCTCGTGCC; CGAATTCGGCACGAGGTCGGACATGGGCAAGAAGCACAAGAAGCACAAGTCGGACAAACA; CCTCTACGAGGAGTATGTAGAGAAGCCCTTGAAGCTGGTCCTCAAAGTAGGAGGGAACGA; AGTCACCGAACTCTCCACGGGCAGCTCGGGGCACGACTCCAGCCTCTTCGAAGACAAAAA; CGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGAAAGGAGAGAAGCAGAT; TCCAGGGGAAGAAAAGGGGAGAAAACGGAGAAGAGTTAAGGAGGATAAAAAGAAGCGAGA; TCGAGACCGGGTGGAGAATGAGGCAGAAAAAGATCTCCAGTGTCACGCCCCTGTGAGATT; AGACTTGCCTCCTGAGAAGCCTCTCACAAGCTCTTTAGCCAAACAAGAAGAAGTAGAACA; GACACCCCTTCAAGAAGCTTTGAATCAACTGATGAGACAATTGCAGAGAAAAGATCCAAG; TGCTTTCTTTTCATTTCCTGTGACTGATTNTATTGCTCCTGGCTACTCCATGATCATTAA; ACACCCAATGGATNTTAGTACCATGAAAGAAAAGATCAAGAACAATGACTATCAGTTCAT; AGAAGAACTAAAGGATAACTTCAAACTAATGTGTACTAATGCCATGATTTACAATAAACC; AGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGAATGAAAATTCTTAG; CCAGGAAAGAATTCAGAGCCTGAAGCAGAGCATAGACTTCATGGCTGACTTGCAGAANAC; TCGAAAGCAGAAAGATGGAACAGACACCTCACANNAGTGGGAGGACNGGAGCTGCTGGCA; NANAGAGAGAGAGACTCTGGAATGCTGAGCACACGCCTTCAGAGTCCCAGCAAGAAAATA; AAAGAAAGACAAGATATGCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_013263 unedited; ATTCTATGNACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGCATGCAAGT; AACTAGGGTTTATTCTATATAATGAATATTTATAGATCTGTAACATTTGTTTCAAAATGC; TGTTTCATTTTTATAAAGTACCAGTGTTTAGCTGCTTTTTATACATTAAATTAGCAATTT; GAAAAACTCAAATATCTGAAGGTATTTTATTTTCGTGGTCCTCATGAACTCACTAGAGAT; TAAAAGTAGACTCAAACAACTTGAAAATTTGACTCTTTTAGCATAAGATTTTAAGTCTTT; TGAGGGAATTAATTTTCCTTTACCCTATTTGTTCTTCCTAAATACTAATTAATTTTCAAA; GTCAGGAAAATAATAGAAATCAAATGGCTTCCTGCCTGGGTGATTTATAGAAGGATTGTA; TAAGGGCCTTCAAACTTAATTTGTTCACTGAACAAGGCTATCTATCTATCTATCTCCATC; CTGATTTTTTTCCCTTGTGTATAACAGGTTCTATACCGATTCAGCAAAATCACCATTTAT; CTCAGCTTGCTGATAAAAGGGGGCCATGAATCTTGTGGTCTCCTACTTAGAGTTCAACTC; CTTGGGTACTTGACCTTTGTATTTCAGATAAACAGTNAGTAAAGTAGAAATGTCACCTTT; TGGTAATCTGGTATTTCCTGATCTATCAAGGATGACCTAGTTTAATCTATTTGGCTTATT; ACTTTGGCATAGTACTAAATAACCTCCTTTTTCTTTAAAAACGGAAAAAACCAAAAAGAA; CACTAGCCATGTGACAGGGCTTTAAAATTCCCATGTGCTTTTGTCAGGGGGGGGGGGAAG; GGCCTAATACCCTTACAAGGGATATTCCCCCGGGTTTCCAGGCCCAACCCCTAGGTAATT; TTAAAATTTTCCAGGTGTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_013263
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013263.2, NP_037395.2
• RefSeq Size: 2204 bp
• RefSeq ORF: 1956 bp
• Locus ID: 29117
• UniProt ID: Q9NPI1
• Cytogenetics: 16q12.1
• Domains: BROMO
• Summary: This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks one internal residue, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.