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BRD7 (NM_013263) Human Untagged Clone

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SKU
SC115318
BRD7 (untagged)-Human bromodomain containing 7 (BRD7), transcript variant 2
  $607.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BRD7
Synonyms BP75; CELTIX1; NAG4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_013263, the custom clone sequence may differ by one or more nucleotides


ATGGGCAAGAAGCACAAGAAGCACAAGTCGGACAAACACCTCTACGAGGAGTATGTAGAGAAGCCCTTGA
AGCTGGTCCTCAAAGTAGGAGGGAACGAAGTCACCGAACTCTCCACGGGCAGCTCGGGGCACGACTCCAG
CCTCTTCGAAGACAAAAACGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGAAAGGAGAG
AAGCAGATTCCAGGGGAAGAAAAGGGGAGAAAACGGAGAAGAGTTAAGGAGGATAAAAAGAAGCGAGATC
GAGACCGGGTGGAGAATGAGGCAGAAAAAGATCTCCAGTGTCACGCCCCTGTGAGATTAGACTTGCCTCC
TGAGAAGCCTCTCACAAGCTCTTTAGCCAAACAAGAAGAAGTAGAACAGACACCCCTTCAAGAAGCTTTG
AATCAACTGATGAGACAATTGCAGAGAAAAGATCCAAGTGCTTTCTTTTCATTTCCTGTGACTGATTTTA
TTGCTCCTGGCTACTCCATGATCATTAAACACCCAATGGATTTTAGTACCATGAAAGAAAAGATCAAGAA
CAATGACTATCAGTCCATAGAAGAACTAAAGGATAACTTCAAACTAATGTGTACTAATGCCATGATTTAC
AATAAACCAGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGAATGAAAATTCTTAGCC
AGGAAAGAATTCAGAGCCTGAAGCAGAGCATAGACTTCATGGCTGACTTGCAGAAAACTCGAAAGCAGAA
AGATGGAACAGACACCTCACAGAGTGGGGAGGACGGAGGCTGCTGGCAGAGAGAGAGAGAGGACTCTGGA
GATGCCGAAGCACACGCCTTCAAGAGTCCCAGCAAAGAAAATAAAAAGAAAGACAAAGATATGCTTGAAG
ATAAGTTTAAAAGCAATAATTTAGAGAGAGAGCAGGAGCAGCTTGACCGCATCGTGAAGGAATCTGGAGG
AAAGCTGACCAGGCGGCTTGTGAACAGTCAGTGCGAATTTGAAAGAAGAAAACCAGATGGAACAACGACG
TTGGGACTTCTCCATCCTGTGGATCCCATTGTAGGAGAGCCAGGCTACTGCCCTGTGAGACTGGGAATGA
CAACTGGAAGACTTCAGTCTGGAGTGAATACTTTGCAGGGGTTCAAAGAGGATAAAAGGAACAAAGTCAC
TCCAGTGTTATATTTGAATTATGGGCCCTACAGTTCTTATGCACCGCATTATGACTCCACATTTGCAAAT
ATCAGCAAGGATGATTCTGATTTAATCTATTCAACCTATGGGGAAGACTCTGATCTTCCAAGTGATTTCA
GCATCCATGAGTTTTTGGCCACGTGCCAAGATTATCCGTATGTCATGGCAGATAGTTTACTGGATGTTTT
AACAAAAGGAGGGCATTCCAGGACCCTACAAGAGATGGAGATGTCATTGCCTGAAGATGAAGGCCATACT
AGGACACTTGACACAGCAAAAGAAATGGAGATTACAGAAGTAGAGCCACCAGGGCGTTTGGACTCCAGTA
CTCAAGACAGGCTCATAGCGCTGAAAGCAGTAACAAATTTTGGCGTTCCAGTTGAAGTTTTTGACTCTGA
AGAAGCTGAAATATTCCAGAAGAAACTTGATGAGACCACCAGATTGCTCAGGGAACTCCAGGAAGCCCAG
AATGAACGTTTGAGCACCAGACCCCCTCCGAACATGATCTGTCTCTTGGGTCCCTCATACAGAGAAATGC
ATCTTGCTGAACAAGTGACCAATAATCTTAAAGAACTTGCACAGCAAGTAACTCCAGGTGATATCGTAAG
CACGTATGGAGTTCGAAAAGCAATGGGGATTTCCATTCCTTCCCCCGTCATGGAAAACAACTTTGTGGAT
TTGACAGAAGACACTGAAGAACCTAAAAAGACGGATGTTGCTGAGTGTGGACCTGGTGGAAGTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_013263 unedited
TTCAACAATTAGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCTCGTGCC
CGAATTCGGCACGAGGTCGGACATGGGCAAGAAGCACAAGAAGCACAAGTCGGACAAACA
CCTCTACGAGGAGTATGTAGAGAAGCCCTTGAAGCTGGTCCTCAAAGTAGGAGGGAACGA
AGTCACCGAACTCTCCACGGGCAGCTCGGGGCACGACTCCAGCCTCTTCGAAGACAAAAA
CGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGAAAGGAGAGAAGCAGAT
TCCAGGGGAAGAAAAGGGGAGAAAACGGAGAAGAGTTAAGGAGGATAAAAAGAAGCGAGA
TCGAGACCGGGTGGAGAATGAGGCAGAAAAAGATCTCCAGTGTCACGCCCCTGTGAGATT
AGACTTGCCTCCTGAGAAGCCTCTCACAAGCTCTTTAGCCAAACAAGAAGAAGTAGAACA
GACACCCCTTCAAGAAGCTTTGAATCAACTGATGAGACAATTGCAGAGAAAAGATCCAAG
TGCTTTCTTTTCATTTCCTGTGACTGATTNTATTGCTCCTGGCTACTCCATGATCATTAA
ACACCCAATGGATNTTAGTACCATGAAAGAAAAGATCAAGAACAATGACTATCAGTTCAT
AGAAGAACTAAAGGATAACTTCAAACTAATGTGTACTAATGCCATGATTTACAATAAACC
AGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGAATGAAAATTCTTAG
CCAGGAAAGAATTCAGAGCCTGAAGCAGAGCATAGACTTCATGGCTGACTTGCAGAANAC
TCGAAAGCAGAAAGATGGAACAGACACCTCACANNAGTGGGAGGACNGGAGCTGCTGGCA
NANAGAGAGAGAGACTCTGGAATGCTGAGCACACGCCTTCAGAGTCCCAGCAAGAAAATA
AAAGAAAGACAAGATATGCTG
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_013263 unedited
ATTCTATGNACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGCATGCAAGT
AACTAGGGTTTATTCTATATAATGAATATTTATAGATCTGTAACATTTGTTTCAAAATGC
TGTTTCATTTTTATAAAGTACCAGTGTTTAGCTGCTTTTTATACATTAAATTAGCAATTT
GAAAAACTCAAATATCTGAAGGTATTTTATTTTCGTGGTCCTCATGAACTCACTAGAGAT
TAAAAGTAGACTCAAACAACTTGAAAATTTGACTCTTTTAGCATAAGATTTTAAGTCTTT
TGAGGGAATTAATTTTCCTTTACCCTATTTGTTCTTCCTAAATACTAATTAATTTTCAAA
GTCAGGAAAATAATAGAAATCAAATGGCTTCCTGCCTGGGTGATTTATAGAAGGATTGTA
TAAGGGCCTTCAAACTTAATTTGTTCACTGAACAAGGCTATCTATCTATCTATCTCCATC
CTGATTTTTTTCCCTTGTGTATAACAGGTTCTATACCGATTCAGCAAAATCACCATTTAT
CTCAGCTTGCTGATAAAAGGGGGCCATGAATCTTGTGGTCTCCTACTTAGAGTTCAACTC
CTTGGGTACTTGACCTTTGTATTTCAGATAAACAGTNAGTAAAGTAGAAATGTCACCTTT
TGGTAATCTGGTATTTCCTGATCTATCAAGGATGACCTAGTTTAATCTATTTGGCTTATT
ACTTTGGCATAGTACTAAATAACCTCCTTTTTCTTTAAAAACGGAAAAAACCAAAAAGAA
CACTAGCCATGTGACAGGGCTTTAAAATTCCCATGTGCTTTTGTCAGGGGGGGGGGGAAG
GGCCTAATACCCTTACAAGGGATATTCCCCCGGGTTTCCAGGCCCAACCCCTAGGTAATT
TTAAAATTTTCCAGGTGTTC
Restriction Sites NotI-NotI |
ACCN NM_013263
Insert Size 3200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013263.2, NP_037395.2
RefSeq Size 2204 bp
RefSeq ORF 1956 bp
Locus ID 29117
UniProt ID Q9NPI1
Cytogenetics 16q12.1
Domains BROMO
Summary This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2010
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks one internal residue, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_013263" in other vectors (6)
SKU Description Size Price
RC208734 BRD7 (Myc-DDK-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 2 10 ug
$606.00
RC208734L1 Lenti ORF clone of Human bromodomain containing 7 (BRD7), transcript variant 2, Myc-DDK-tagged 10 ug
$906.00
RC208734L2 Lenti ORF clone of Human bromodomain containing 7 (BRD7), transcript variant 2, mGFP tagged 10 ug
$906.00
RC208734L3 Lenti ORF clone of Human bromodomain containing 7 (BRD7), transcript variant 2, Myc-DDK-tagged 10 ug
$906.00
RC208734L4 Lenti ORF clone of Human bromodomain containing 7 (BRD7), transcript variant 2, mGFP tagged 10 ug
$906.00
RG208734 BRD7 (tGFP-tagged) - Human bromodomain containing 7 (BRD7), transcript variant 2 10 ug
$489.00 $806.00

file-alt Citations

Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in transcriptional repression of their target genes
Tae, Karkhanis, Velasco et al
Nucleic Acids Res. (2011) 39 (13), 5424-38 DOI: 10.1093/nar/gkr170
Cited by 92
AI Summary: OriGene’s pCMV6-XL5/BRD7 vector was used as the source of BRD7 cDNA for cloning and protein expression in this study, enabling functional analyses of BRD7’s role in transcriptional repression.
Snippet: a 1.9-kb BRD7 cDNA-encoding amino acids 2–651, which was PCR-amplified from the pCMV6-XL5/BRD7 vector (Origene Technologies Inc ... Plasmid pGEX-2TK/BRD7 (amino acids 2–651), which expresses GST–BRD7 used in GST pull-down assays and antibody production
FigureFigureFigure
Showing 1-1 of 1 papers.
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