IKBKE (NM_014002) Human Untagged Clone

SKU SC115206

IKBKE (untagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IKBKE
• Synonyms: IKK-E; IKK-i; IKKE; IKKI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014002 edited; ATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACT; GCCAGTGTGTACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGAAGGTCTTC; AACACTACCAGCTACCTGCGGCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGG; AAGCTGAACCACCAGAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGG; CAGAAGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGC; CCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCGCTGTGTGGTG; GCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGGGAAC; ATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCT; GCCCGGGAGCTGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTG; CATCCCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTG; ACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGCCACTGGCAGCCTGCCC; TTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCATGTACCGGATCACCACGGAG; AAGCCGGCTGGGGCCATTGCAGGTGCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGC; TACACCCTCCCCATCACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATC; CTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCG; GAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGGCAGTC; CTGCACCACATCTATATCCATGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCAC; AAGCAGACCAGTGTGGCCCCCCGACACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTC; CTCGAGCCCAGCGTCTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACC; CTCTTCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGACGTCCCC; AAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGCGTGTTGGGC; GCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGGCAGGAGCTAATGTTT; CGGGGGCTGCACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTG; GCAAGGACATCCCTCCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTG; GCTGGAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGGCTGCGG; ACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGAGACCCAGGAGAGCCTG; AGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGTACATGAGGACAGAAGCATC; CAGCAGATTCAGTGCTGTTTGGACAAGATGAACTTCATCTACAAACAGTTCAAGAAGTCT; AGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAAT; TTCAGTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTAT; CAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCAC; CTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTAACACAGAAGCCCAGGGGGTCCAGGAG; AGTCTCAGCAAGCTCCTGGAAGAGCTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGG; GCTCAGGCCTCGCCGCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTT; CTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAAC; AACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACTTCCTGATGTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014002 unedited; AGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGNAAAAAACCC; ACTAGTCCCAGCTCCTGGGGTGGCACAGACATTGCAACTGGCCCTGCCTGTGGGTCCTAG; GGGCCCTTGGCTACCAGGAGGCTAAGAACACTGCTCATGAATGACAGTGAGCCCTGAAAG; CTCTGGGGGTGTCACCCAGTCCCACAAGCCTGCATCCCCTGCAGTGGAGATGGGCTCAGC; TCCTGGACGTGCCACAGACAGAAAGCATAACATACACTCGCCAGGAAGAGCCTTTGCCTG; ACTCAGGGCAGCTCAGAGTGTGGGGCAGAAGGTGACCAGCCAGCTCAGGGCAGGAGATGC; AGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACTGCCA; GTGTGTACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGAAGGTCTTCAACA; CTACCAGCTACCTGCGGCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGC; TGAACCACCAGAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGA; AGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGCCCTG; AGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCGCTGTGTGGTGGCCG; GCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGNGACATCAT; GCGCCTNCCTTAAGGGAAGGAGGGGCAGAGCATCTACNAGCTGACAGACTTTCGCGCTGC; CCGGGAGCTGGATGATGATGAGAAGTTTCGTCTCGTCTATGGGACTGAGGAGTACCTGCA; TCCCNGAATGTATGAGCCGGGCGTGCTTTCAAAGCCNCCAGCAAAAAGCGTCGNGTGACT; GTTGGATCTCTGGAGCATGGANTGACCTTGTACAGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014002 unedited; AGTCTTGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTAACAAAAGCAA; AGGCCACATTTATTCCACTTCTTGAAGGTGCAGGCCTTGGACCTGCCCAGACTCACAGTC; CTGGCTGCATGTCCCAGGACCAGCTGGAGCCTCAGAGAGTGAGGGAGAGCCAAAGGGGCC; CCAAGACACTGGCACTGGCCTTGGCCACCCATTTTCTCAACTTTAGGCTCAGTCAGACCA; AGCATAGAAAGAACAGGAGGCTCTCTATGTTGCCTGAGGCTGGCAGTGGTGACCCTCAGA; AATCACAAGGGAGCGGAGAGAGCTGTGTTCAACATGAAACGCTTGAAGAGGAAGGAAGAC; TGTGATGAGGTCGTTTGACCTACTGCCCTGGGGAAACCCCAGGAGGCCTCCTCTCCTGTT; GGCTAGGGAGTGAAGTCCTATAAACCAGAGGGAGTGGGAAGGCGTATGAGAGCTTCTTCT; CCCCACGGCCCAGCCATGGGCATGGAGGAGCAGTACCTGAGCCTAGAGAAGATAGAAAGG; TCAGCCAGGCAGTTGCTGCCACACTCTTTCCTGTGGGTCCCAACAGAGGTGGCCTGGTGC; CCAGTCTCAGCTGTGCTGCTTCCCAGGGAGAAAGGCAGTGGAAGGTAATGCTGGCGACAT; CCTGGCCAGCAGCCAGGAGGGAGGTAGGCTGATGTGATGGGAATGGGATCTTGCCCTGGG; TTGGTCTCATGGTGCAGAAGAGCAGTGTTGGAATCATTCTAATGCTTCAGGATGCCTCAT; GTGCCCCATGGAGCTCAGACATCAGGAAGTGCTGGGACTCTATTTAGCCGTCGATGATGC; GGTTTGTGTCCAAGAGTCAGATGCCGCAGCTTCATCCCTCGCAGACTCTGCATGTGGAGA; GCAGTCCTTTCTGTAGGCTTGGGTAAGGACTATGGGAGCGGCCAGCCTGACCCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014002
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014002.2, NP_054721.1
• RefSeq Size: 3263 bp
• RefSeq ORF: 2151 bp
• Locus ID: 9641
• UniProt ID: Q14164
• Cytogenetics: 1q32.1
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Summary: IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]; Transcript Variant: This variant (1) is the longest transcript and it encodes the longest protein (isoform 1).