MRPL18 (NM_014161) Human Untagged Clone

SKU SC115152

MRPL18 (untagged)-Human mitochondrial ribosomal protein L18 (MRPL18), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: MRPL18
• Synonyms: HSPC071; L18mt; MRP-L18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014161 edited; ATGGCGCTTCGGTCGCAGTTTTGGGGGTTCTCGGTTTGCAGGAACCCTGGGTGCAGGTTC; GCAGCCCTGTCAACCAGCTCCGAGCCGGCAGCGAAACCTGAAGTGGACCCTGTGGAAAAT; GAAGCTGTCGCCCCAGAATTCACCAACCGGAACCCCCGGAACCTGGAGCTTTTATCTGTA; GCCAGGAAAGAGCGGGGCTGGCGGACGGTGTTTCCCTCCCGTGAGTTCTGGCACAGGTTG; CGAGTTATAAGGACTCAGCATCATGTAGAAGCACTTGTGGAGCATCAGAATGGCAAGGTT; GTGGTTTCGGCCTCCACTCGTGAGTGGGCTATTAAAAAGCACCTTTATAGTACCAGAAAT; GTGGTGGCTTGTGAGAGTATAGGACGAGTGCTGGCACAGAGATGCTTAGAGGCGGGAATC; AACTTCATGGTCTACCAACCAACCCCGTGGGAGGCAGCCTCAGACTCGATGAAACGACTA; CAAAGTGCCATGACAGAAGGTGGTGTGGTTCTACGGGAACCTCAGAGAATCTATGAATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014161 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGCGAGCGACTGAG; TCGTCCGTGAGGAAAAAGAGGCGAGGCTTTTCCGAGATCGTCTCAGCGATGGCGCTTCGG; TCGCAGTTTTGGGGGTTCTCGGTTTGCAGGAACCCTGGGTGCAGGTTCGCAGCCCTGTCA; ACCAGCTCCGAGCCGGCAGCGAAACCTGAAGTGGACCCTGTGGAAAATGAAGCTGTCGCC; CCAGAATTCACCAACCGGAACCCCCGGAACCTGGAGCTTTTATCTGTAGCCAGGAAAGAG; CGGGGCTGGCGGACGGTGTTTCCCTCCCGTGAGTTCTGGCACAGGTTGCGAGTTATAAGG; ACTCAGCATCATGTAGAAGCACTTGTGGAGCATCAGAATGGCAAGGTTGTGGTTTCGGCC; TCCACTCGTGAGTGGGCTATTAAAAAGCACCTTTATAGTACCAGAAATGTGGTGGCTTGT; GAGAGTATAGGACGAGTGCTGGCACAGAGATGCTTAGAGGCGGGAATCAACTTCATGGTC; TACCAACCAACCCCGTGGGAGGCAGCCTCAGACTCGATGAAACGACTACAAAGTGCCATG; ACAGAAGGTGGTGGTGGTTCTACGGGAACCTCAGAGAATCTATGAATAAATGGAAGCATT; AATTGTTNTGAACATGTAAATATAAATCTGTCAGCCACTACAGCCATCAAAAGAGAGCAT; CTGGAAGAACAGCCAGCTTTGGAAAGTTTACAGCAATAATGGTTGCAGTGGAATATTATN; TGTAGNTAAGGTCATCCTCCTNCCCTTTTCTGTTTTTAAAATCAAGACTACATTCTGNNC; CCTCTCTGGGCTTCAGAGCATCTAGAANAGCAGNCATCAATATAATTAACTTTCAAGGGG; CAGTCANAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014161 unedited; TACTATGNACGCGGCCGCATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTAAAAGTTCA; TAATATGCCTTTATTTTGTAATTTATAAACAACTTCTGACTTGCCCTTTGAAAGTTAATT; ATAATTGATGACTGCTTTTCTTAAATGCTTCTGAAGCCCAAGAGAGGGGCAGAATGTAGT; TCTTGATTTAAAAAAACAGAAAGGGGAGGAGGATGACCTTAACTACAAATAATATTCCAC; TGCAACATTATTGCTGTAAAACTTCCAAGCTGGCTGTTCTTCCAAATGCTCTCTTTTGAT; GGCTGTAGTGGCTGACAGATTTATATTTACATGTTCAAAACAATTAATGCTTCCATTTAT; TCATAGATTCTCTGAGGTTCCCGTAGAACCACACCACCTTCTGTCATGGCACTTTGTAGT; CGTTTCATCGAGTCTGAGGCTGCCTCCCACGGGGTTGGTTGGTAGACCATGAAGTTGATT; CCCGCCTCTAAGCATCTCTGTGCCAGCACTCGTCCTATACTCTCACAAGCCACCACATTT; CTGGTACTATAAAGGTGCTTTTTAATAGCCCACTCACGAGTGGAGGCCGAAACCACAACC; TTGCCATTCTGATGCTCCACAAGTGCTTCTACATGATGCTGAGTCCTTATAACTCGCAAC; CTGTGCCAGAACTCACGGGAGGGAAACACCGTCCGCCAGCCCCGCTCTTTCCTGGCTACA; GATAAAAGCTCCAGGTTCCGGAGGTTCCGGTTGGTGAATTCTGGGGCGACAGCTTCATTT; TCCACAGGGTCCACTTNCAGTTTCGCTGCCAGCTCGNAGCTGGTTGACAGGGCTGCGAAC; CTGCACCCAAGGTTCCTTGCAACCGAAATCCCCAAACTGCGACCGAACGCATCGCTGAGA; GATCTCGGAAAGCTCGCTCTTTCCTCACGACATCTATCGCTCGCACTCGTGCGATTCCGG; CCGCCTTATGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014161
• Insert Size: 970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014161.2, NP_054880.2
• RefSeq Size: 989 bp
• RefSeq ORF: 543 bp
• Locus ID: 29074
• UniProt ID: Q9H0U6
• Cytogenetics: 6q25.3
• Summary: This nuclear gene encodes a protein component of the larger 39S subunit of mitochondrial ribosome. This protein may also aid in the import of nuclear-encoded 5S rRNA into mitochondria. Alternative splicing results in multiple transcript variants, most of which are not predicted to encode a protein. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) encodes the longer isoform (1).