NDUFA8 (NM_014222) Human Untagged Clone

SKU SC115137

NDUFA8 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa (NDUFA8), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFA8
• Synonyms: CI-19KD; CI-PGIV; MC1DN37; PGIV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014222 edited; ATGCCGGGGATAGTGGAGCTGCCCACTCTAGAGGAGCTGAAAGTAGATGAGGTGAAAATT; AGTTCTGCTGTGCTTAAAGCTGCGGCCCATCACTATGGAGCTCAATGTGATAAGCCCAAC; AAGGAATTTATGCTCTGCCGCTGGGAAGAGAAAGATCCGAGGCGGTGTTTAGAGGAAGGC; AAACTGGTCAACAAGTGTGCTTTGGACTTCTTTAGGCAGATAAAACGTCACTGTGCAGAG; CCTTTTACAGAATATTGGACTTGCATTGATTATACTGGCCAGCAGTTATTTCGTCACTGT; CGCAAACAGCAGGCAAAGTTTGACGAGTGTGTGCTGGACAAACTGGGCTGGGTGCGGCCT; GACCTGGGAGAACTGTCAAAGGTCACCAAAGTGAAAACAGATCGACCTTTACCGGAGAAT; CCCTATCACTCAAGACCAAGACCGGATCCCAGCCCTGAGATCGAGGGAGATCTGCAGCCT; GCCACACATGGCAGCCGCTTTTATTTCTGGACCAAGTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014222 unedited; GTTTGNAATTTGTAATACGACTCACTATAGGNGCGGNNCGCGAATCGGCACGAGGCGGGG; GCGACGCGGCTGAGGGCTTCTCGTCGGGGTCGGGGCTGCAGCCGTCATGCCGGGGATAGT; GGAGCTGCCCACTCTAGAGGAGCTGAAAGTAGATGAGGTGAAAATTAGTTCTGCTGTGCT; TAAAGCTGCGGCCCATCACTATGGAGCTCAATGTGATAAGCCCAACAAGGAATTTATGCT; CTGCCGCTGGGAAGAGAAAGATCCGAGGCGGTGTTTAGAGGAAGGCAAACTGGTCAACAA; GTGTGCTTTGGACTTCTTTAGGCAGATAAAACGTCACTGTGCAGAGCCTTTTACAGAATA; TTGGACTTGCATTGATTATACTGGCCAGCAGTTATTTCGTCACTGTCGCAAACAGCAGGC; AAAGCTTGACGAGTGTGTGCTGGACAAACTGGGCTGGGTGCGGCCTGACCTGCGAGAACT; GCCAAAGGTCACCAAAGTGAAAACAGATCGACCTTTACCGGAGAATCCCTATCACTCAAG; ACCAAGACCGGATCCCAGCCCTGAGATCGAGGGAGATCTGCAGCCTGCCACACATGGCAG; CCGCTTTTATTTCTGGACCAAAGCAAAGATGGGTCCGTGGCCCACACTCGGGTCATGTGC; TCAGAACACGACTGATGAAAACCGCCCATGCGGTTTGCATCGACTGATAGTGCGCTCCTT; TCCGGGACACAAACATTAACAAAAAATTAACCTATGTGACTTGGCCGTTATTCCATACCA; TCTCCTCGCCATAAAATATTTAAAGGGCCACAAATCCCCCACCGCACCACACCCCCCCCC; CCCCCCCCCCCCCCCCAGCCCCCCCCCCCCCGCACACCCCCCCCCCCCCCCCACCCCAAA; AACACCCCCCCTCTTAGCGGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014222 unedited; CTTTTCATGGCCAGGTAAAGGTATAGNATAACTGCCAAGTCACATAAGTTAACTTTTTTG; TTAATGGTTTGATGATCCCGGNAAATAACACACTATCAGTCGATGCAAACCGCATGGGCG; TTTTCATCAGTCGTTGTCTGAGCACATGACCGAGTGTGGGCCACGGACCCATCTTTACTT; GGTCCAGAAATAAAAGCGGCTGCCATGTGTGGCAGGCTGCAGATCTCCCTCGATCTCAGG; GCTGGGATCCGGTCTTGGTCTTGAGTGATAGGGATTCTCCGGTAAAGGTCGATCTGTTTT; CACTTTGGTGACCTTTGACAGTTCTCCCAGGTCAGGCCGCACCCATCCCAGTTTGTCCAG; CACACACTCGTCAAACTTTGCCTGCTGTTTGCGACAGTGACGAAATAACTGCTGGCCAGT; ATAATCAATGCAAGTCCAATATTCTGTAAAAGGCTCTGCACAGTGACGTTTTATCTGCCT; AAAGAAGTCCAAAGCACACTTGTTGACCAGTTTGCCTTCCTCTAAACACCGCCTCGGATC; TTTCTCTTCCCAGCGGCAGAGCATAAATTCCTTGTTGGGCTTATCACATTGAGCTCCATA; GTGATGGGCCGCAGCTCTAAGCACAGCAGAACTAATTTTTACCCTCATCTACTTTTCAGC; TCCTCTAGAGTGGGCAGCTCCACTATCCCCGGCATGACAGCTGCAGCCCCGATCCCGACG; AGTATCCCTTATCCGCGTTGTCCCCCGCCTTGTGCCGTATTTCCCGGCCGCCCTATATGG; AGTCGGTTTACAAAATTCTGACGGTTCACTAAACCAGCCTTGCTTATATAACCTTCCACC; GTCACGCCTACCGCCTTTTGGCAAACGGGGCGAGTTTTACAACATTTTGAAAGTCCGCTG; TTTTGGTGCCAAACAACTCCATGACGCCATGGGGGGAAACTTGTAATCCCGGGGGTAAAC; GCTTCACCGCCTTGGGGGCCTGCCAACCCTTCCCATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014222
• Insert Size: 870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014222.2, NP_055037.1
• RefSeq Size: 859 bp
• RefSeq ORF: 519 bp
• Locus ID: 4702
• UniProt ID: P51970
• Cytogenetics: 9q33.2
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).