ARNT2 (NM_014862) Human Untagged Clone

SKU SC114814

ARNT2 (untagged)-Human aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARNT2
• Synonyms: bHLHe1; WEDAS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114814 sequence for NM_014862 edited (data generated by NextGen Sequencing); ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG; ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT; GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC; AGTAAATTTTCANNNGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT; CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA; GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG; AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT; CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG; ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG; AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC; ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA; GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG; ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG; AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC; CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC; GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC; AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG; CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC; CAACCCCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC; CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG; TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC; CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA; CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC; TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG; GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA; GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG; ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA; GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG; TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC; TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT; GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT; ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT; GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG; CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG; ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAACCCAGGGGACTGGCAAC; TATAACATCGAAGACTTTGCCGACCTGGGCATGTTTCCACCGTTTTCTGAGTAG; ; Clone variation with respect to NM_014862.3; 193 a=>n;194 g=>n;195 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014862 unedited; GTAACGTTCCAATTTGTATACGACTCATATAGGCGGCCGCGAATTCGCACGAGGGGAGCG; CCGCCCGCCCGCGCCGTCCTTTGTGTGGCGGCGGCGGCGCCTGGTCCTGACCGGGTCCCC; GGGGCTGAGCGCCGGGCTCCGCGCCGCCCCTCCCGCGCCCCTGCCAAGCGGGCGCCTATC; CTCTCCGAGCAAGATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACAT; ACCTGGATCTGTGACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGC; GGGGGCCATGCCTGCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGA; TGGTGAAGGCCCCAGTAAATTTTCAAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGA; ACAAGATGACTCAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGG; CTCGGAAGCCAGACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGA; GGGGTACAGGGAACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGG; AACTGAAGCATCTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGA; CAGGGCGAGTGATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAG; AGTGGTTTGGGAGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAG; AGCAACTGTGCACCTCAGATAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGA; CGGTCAAGATAGAATGGCAGCAGTCATCCATGAAGATGTGCATGGGCTCGCGGCGGTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014862 unedited; ATGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTAAAGGAAACTGAGATT; GTTAGATGAAGCAAGCCGTCCTGCTCCCGCACAGCCTGTGAAACCTCCATTTTGCCACTT; TCAAGGTCAGTGCCCCACAGACCCTGGCCTGTTGTTGACCATAACACTAGCTTTGGCCAC; AAAAGCCAGGGACACCTACGCTTGGAAATGTGCCTGCACCACTGTGTGGTGCCTCCTTAA; GTTGCCCCACACTCCACATGGCCAAAGAAAAGGTGAATGTCCAGGTGGTTTATTGGCTTC; TTATTATGGGGTGTTTGGTGGGGAATGACCCCGGTAGGTTAGGAGACAGTGACTTAGGTC; AAGTCCATATTTTCTGGTTTTATAAAGGACACTCGTATTCTGACTTGCGATTTCTATAGC; TGTAAGGGTTACTGTCTGTTTACTGTTATATGTGGTTCGCTATAACCGTCACGGTATTAG; GTGAGTGCTCCATTGTTATGTTCCTTATGGACTCTTTAAGTGCCGTCCTCAGTGGTCAGA; CTATTTCTTTATTTGAATATGTTGTCTTTTTTGTTTTTGCCCTTAAGTCTTCATGTTATT; CTGTATTCATCGCATCCCGTAATCACATCTAAATATCTTCTCCTCTTCTNCTCACTTCTC; TCTCTCTCTTATCACCTCGATTTCGCCCCTCACCTCTTCCTTCCTTCTGCTATTTCTCCA; TATGCTCTCTCCTTCATCTCTCTATGTGTTCTCCCCTCTTTGCCCTTCATTTCGCTTCCC; CCTTCTTCTTCTTCCTCATCTTCTCTTTATTCTTCTTCTGCACCCCTCGCTCCNCACTAT; CTTACTCTTTCCTCTTCCTAATTCTTTTTTCACACTCACCCTCCTCTACTTCTCTCTCTC; TTGTCCTGATCTTGTCATCCTATTAGTGATTCCTCATCTTATCATTTCCTACCTTCGATT; CTAATCTACCTACACCCCCTCCTCTTCGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014862
• Insert Size: 6930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014862.3, NP_055677.3
• RefSeq Size: 6576 bp
• RefSeq ORF: 2154 bp
• Locus ID: 9915
• UniProt ID: Q9HBZ2
• Cytogenetics: 15q25.1
• Domains: HLH, PAC, PAS
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Summary: This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]