ENPP4 (NM_014936) Human Untagged Clone

SKU SC114773

ENPP4 (untagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative) (ENPP4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ENPP4
• Synonyms: NPP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114773 sequence for NM_014936 edited (data generated by NextGen Sequencing); ATGAAGTTATTAGTAATACTTTTGTTTTCTGGACTTATAACTGGTTTTAGAAGTGACTCT; TCCTCTAGTTTGCCACCTAAGTTACTACTAGTATCCTTTGATGGCTTCAGAGCTGATTAT; CTGAAGAACTATGAATTTCCTCATCTCCAGAATTTTATCAAAGAAGGTGTTTTGGTAGAG; CATGTTAAAAATGTTTTTATCACAAAAACATTTCCAAACCACTACAGTATTGTGACAGGC; TTGTATGAAGAAAGCCATGGCATTGTGGCTAATTCCATGTATGATGCAGTCACAAAGAAA; CACTTTTCTGACTCTAATGACAAGGATCCTTTTTGGTGGAATGAGGCAGTACCTATTTGG; GTGACCAATCAGCTTCAGGAAAACAGATCAAGTGCTGCTGCTATGTGGCCTGGTACTGAT; GTACCCATTCACGATACCATCTCTTCCTATTTTATGAATTACAACTCCTCAGTGTCATTT; GAGGAAAGACTAAATAATATTACTATGTGGCTAAACAATTCGAACCCACCAGTCACCTTT; GCAACACTATATTGGGAAGAACCAGATGCAAGTGGCCACAAATACGGACCTGAAGATAAA; GAAAACATGAGCAGAGTGTTGAAAAAAATAGATGATCTTATCGGTGACTTAGTCCAAAGA; CTCAAGATGTTAGGGCTATGGGAAAATCTTAATGTGATCATTACAAGTGATCATGGGATG; ACCCAGTGTTCTCAGGACAGACTGATAAACCTGGATTCCTGCATCGATCATTCATACTAC; ACTCTTATAGATTTGAGCCCAGTTGCTGCAATACTTCCCAAAATAAATAGAACAGAGGTT; TATAACAAACTGAAAAACTGTAGCCCTCATATGAATGTTTATCTCAAAGAAGACATTCCT; AACAGATTTTATTACCAACATAATGATCGAATTCAGCCCATTATTTTGGTTGCCGATGAA; GGCTGGACAATTGTGCTAAATGAATCATCACAAAAATTAGGTGACCATGGTTATGATAAT; TCTTTGCCTAGTATGCATCCATTTCTAGCTGCCCACGGACCTGCATTTCACAAAGGCTAC; AAGCATAGCACAATTAACATTGTGGATATTTATCCAATGATGTGCCACATCCTGGGATTA; AAACCACATCCCAATAATGGGACCTTTGGTCATACTAAGTGCTTGTTAGTTGACCAGTGG; TGCATTAATCTCCCAGAAGCCATCGCGATTGTTATCGGTTCACTCTTGGTGTTAACCATG; CTAACATGCCTCATAATAATCATGCAGAATAGACTTTCTGTACCTCGTCCATTTTCTCGA; CTTCAGCTACAAGAAGATGATGATGATCCTTTAATTGGGTGA; ; Clone variation with respect to NM_014936.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014936 unedited; AGTTAACATTTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGGCCGCGAGC; GCCGAGTTCCGCGCATTAGGAAATAAGCGACCGCGGCGGCTGGAACCCTGATTGCTGTCC; TTCAACGTGTTCATTATGAAGTTATTAGTAATACTTTTGTTTTCTGGACTTATAACTGGT; TTTAGAAGTGACTCTTCCTCTAGTTTGCCACCTAAGTTACTACTAGTATCCTTTGATGGC; TTCAGAGCTGATTATCTGAAGAACTATGAATTTCCTCATCTCCAGAATTTTATCAAAGAA; AGTGTTTTGGTAGAGCATGTTAAAAATGTTTTTATCACAAAAACATTTCCAAACCACTAC; AGTATTGTGACAGGCTTGTATGAAGAAAGCCATGGCATTGTGGCTAATTCCATGTATGAT; GCAGTCACAAAGAAACACTTTTCTGACTCTAATGACAAGGATCCTTTTTGGTGGAATGAG; GCAGTACCTATTTGGGTGACCAATCAGCTTCAGGAAAACAGATCAAGTGCTGCTGCTATG; TGGCCTGGTACTGATGTACCCATTCACGATACCATCTCTTCCTATTTTATGAATTACAAC; TCCTCAGTGTCATTTGAGGAAAGACTAAATAATATTACTATGTGGCTAAACAATTCGAAC; CCACCAGTCACCTTTGCAACACTATATTGGGAAGAACCAGATGCAAGTGGCCACAAATAC; GGACCTGAAGATAAAGAANACATGAGCAGAGTGTTGAAAAAATAGATGATCTTATCGGTG; ACTTAGTCCAAGACTCAAGATGTTAGGGCTATGGGGAAAATCTAATGTGATCATTANCAG; TGATCATGGGATGACCCAGTGNNTCTCAGNACAGACTGATAACTGGGATTCTGCATCGAT; CATTCATACTACACTCTTATAGATTTTGAGCCCCAGTGCTGCATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014936 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATATAATATATGTTTT; ATTAAATAAATACTATTCTTCTTGTCCCATCAACATATACTTTTTAAATTTTTGCTTAAT; TTACAATTCGAGTTAATATTTTGAGAACGTAAGGAAAGACAAATAGGTAAATGTCTTGTT; GAAAGGGTTAAGATACTGACAGGACATACCTTCCTGTTCCATCCTATATAAAATCCACTC; ATGAGTTGGATGCCAAGTATAGATTTCTGAAACTCATAACCACCAATCACGATAATGCAA; TCATTAAAACTCACACCATTGCAAGGACCAAGTATAAAATATTCTGCTACTATTTTAGAT; ACTTCAAAAACATCCACTACTTTAAATGAAAAGCTTTAAATGTGTCAATGCTATTTGCTT; TTTAATGGAAATTAAAGTAGAATTTTTCTATTATTCTGTTTCGATCAACTATTAACCTTT; AGGATAATTGTTTTATGTGAAAAAAATTAGAAATTTTTTTAAAGCAAAATTAGTACTATT; GGTTTTTATTTATTTTACTGTTCTTAATACAGTTAACTTATGACCAACAGCATGGATTAT; TTATACAGGGTTTGAAAATGCCTCTCAACAACCACAAATACCATAGGCAGCAAATACCAG; GCTCAAGAAATTAGCAGTGACTCAATAGGACGCACTGTATGTTTGATGACTGGGCACCAC; TGTTGTCTTTACATCACAGGATATCAAATTTAACTGGGCTGCATATTATTATACTTTTAT; ATTTGTAAACAGTTACCAGGTTTATTTATATTGTCAAAACTTAAAGATCATCTATTACTG; CCTGCTCACGTCTGATGATGGTCTTGACCCCATTTTTCCCTACTATTTAATCTAGTCGGA; CAACCTAGTTCTCATATTATTTTGATACAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014936
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014936.3, NP_055751.1
• RefSeq Size: 4312 bp
• RefSeq ORF: 1362 bp
• Locus ID: 22875
• UniProt ID: Q9Y6X5
• Cytogenetics: 6p21.1
• Domains: Phosphodiest
• Protein Families: Transmembrane
• Summary: Hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. Acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.[UniProtKB/Swiss-Prot Function]