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SAMHD1 (NM_015474) Human Untagged Clone

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SKU
SC114650
SAMHD1 (untagged)-Human SAM domain and HD domain 1 (SAMHD1)
  $584.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SAMHD1
Synonyms CHBL2; DCIP; HDDC1; hSAMHD1; MOP-5; SBBI88
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114650 sequence for NM_015474 edited (data generated by NextGen Sequencing)
ATGCAGCGAGCCGATTCCGAGCAGCCCTCCAAGCGTCCCCGTTGCGATGACAGCCCGAGA
ACCCCCTCAAACACCCCTTCCGCAGAGGCAGACTGGTCCCCGGGCCTGGAACTCCATCCC
GACTACAAGACATGGGGTCCGGAGCAGGTGTGCTCCTTCCTCAGGCGCGGTGGCTTTGAA
GAGCCGGTGCTGCTGAAGAACATCCGAGAAAATGAAATCACAGGCGCATTACTGCCTTGT
CTTGATGAGTCTCGTTTTGAAAATCTTGGAGTAAGTTCCTTGGGGGAGAGGAAGAAGCTG
CTTAGTTATATCCAGCGATTGGTTCAAATCCACGTTGATACAATGAAGGTAATTAATGAT
CCTATCCATGGCCACATTGAGCTCCACCCTCTCCTCGTCCGAATCATTGATACACCTCAA
TTTCAACGTCTTCGATACATCAAACAGCTGGGAGGTGGTTACTATGTTTTTCCAGGAGCT
TCACACAATCGATTTGAGCATAGTCTAGGGGTGGGGTATCTAGCAGGATGTCTAGTTCAC
GCACTGGGTGAAAAACAACCAGAGCTGCAGATAAGTGAACGAGATGTTCTCTGTGTTCAG
ATTGCTGGACTTTGTCATGATCTCGGTCATGGGCCATTTTCTCACATGTTTGATGGACGA
TTTATTCCACTTGCTCGCCCGGAGGTGAAATGGACGCATGAACAAGGCTCAGTTATGATG
TTTGAGCACCTTATTAATTCTAATGGAATTAAGCCTGTCATGGAACAATATGGTCTCATC
CCTGAAGAAGATATTTGCTTTATAAAGGAACAAATTGTAGGACCACTTGAATCACCTGTC
GAAGATTCATTGTGGCCATATAAAGGGCGTCCTGAAAACAAAAGCTTCCTTTATGAGATA
GTATCTAATAAAAGAAATGGCATTGATGTGGACAAATGGGATTATTTTGCCAGGGACTGC
CATCATCTTGGAATCCAAAATAATTTTGATTACAAGCGCTTTATTAAGTTTGCCCGTGTC
TGTGAAGTAGACAATGAGTTGCGTATTTGTGCTAGAGATAAGGAAGTTGGAAATCTGTAT
GACATGTTCCACACTCGCAACTCTTTACACCGTAGAGCTTATCAACACAAAGTTGGCAAC
ATTATTGATACAATGATTACAGATGCTTTCCTCAAAGCAGATGACTACATAGAGATTACA
GGTGCTGGAGGAAAAAAGTATCGCATTTCTACAGCAATTGACGACATGGAAGCCTATACT
AAGCTGACAGATAACATTTTTCTGGAGATTTTATACTCTACTGATCCCAAATTGAAAGAC
GCACGAGAGATTTTAAAACAAATTGAATACCGTAATCTATTCAAGTATGTGGGTGAGACG
CAGCCAACAGGACAAATAAAGATTAAAAGGGAGGACTATGAATCTCTTCCAAAAGAGGTT
GCCAGTGCTAAACCCAAAGTATTGCTAGACGTGAAACTGAAGGCTGAAGATTTTATAGTG
GATGTTATCAACATGGATTATGGAATGCAAGAAAAGAATCCAATTGATCATGTTAGCTTC
TATTGTAAGACTGCCCCCAACAGAGCAATCAGGATTACTAAAAACCAGGTTTCACAACTT
CTGCCAGAGAAATTTGCAGAGCAGCTGATTCGAGTATATTGTAAGAAGGTGGACAGAAAG
AGTTTGTATGCCGCAAGACAATATTTTGTTCAGTGGTGTGCAGACAGAAATTTCACCAAG
CCGCAGGATGGCGATGTTATAGCCCCACTCATAACACCTCAAAAAAAGGAATGGAACGAC
AGTACTTCAGTCCAAAATCCAACTCGCCTCCGAGAAGCATCCAAAAGCAGAGTCCAGCTT
TTTAAAGATGACCCAATGTGA

Clone variation with respect to NM_015474.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_015474 unedited
GGGTGGTCAGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGGGCCC
TGCTGTCAGTGCGCCTGCGCGCGGGTCCGGCGCCGAGGTTCTTGACTGCTGTGCCGGACG
CCAGGTGTAGCCATGCAGCGAGCCGATTCCGAGCAGCCCTCCAAGCGTCCCCGTTGCGAT
GACAGCCCGAGAACCCCCTCAAACACCCCTTCCGCAGAGGCAGACTGGTCCCCGGGCCTG
GAACTCCATCCCGACTACAAGACATGGGGTCCGGAGCAGGTGTGCTCCTTCCTCAGGCGC
GGTGGCTTTGAAGAGCCGGTGCTGCTGAAGAACATCCGAGAAAATGAAATCACAGGCGCA
TTACTGCCTTGTCTTGATGAGTCTCGTTTTGAAAATCTTGGAGTAAGTTCCTTGGGGGAG
AGGAAGAAGCTGCTTAGTTATATCCAGCGATTGGTTCAAATCCACGTTGATACAATGAAG
GTAATTAATGATCCTATCCATGGCCACATTGAGCTCCACCCTCTCCTCGTCCGAATCATT
GATACACCTCAATTTCAACGTCTTCGATACATCAAACAGCTGGGAGGTGGTTACTATGTT
TTTCCAGGAGCTTCACACAATCGATTTGAGCATAGTCTAGGGGTGGGGTATCTAGCAGGA
TGTCTAGTTCACGCACTGGGTGAAAAACAACCAGAGCTGCAGATAAGTGAACGAGATGTT
CTCTGTGTTCAGATTGCTGGACTTTGTCATGATCTCGGTCATGGGCCATTTTCTCACATG
TTTGATGGACGATTTATTCCACTTGCTCGCCCGGNAGGTGAAATGGACGCATGAACAAGG
CTCAGTTATGATGTTTGAGCACCTTATTAATTCTATGGGATNAGCCTGTCATGGACN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_015474 unedited
NANGATCGAGTTTTTTTTTTTTTTTTTTGAAGGAGAAAGCTTTAATAATATTAAAAATAG
CAAGGTAATGCTTTTCATAGTATAGTAAGATTTGCCTAATACCATCTTATTTCTTTGATT
AAAAGTTACTTAGCTTCAGCATGCGTGTACATTTAAAATACAAAATTAAAGCATGAGTTG
TCATTAATTTGCAGAATTCTATGATTGAAGCCTCTAAATGAATTGTGCAGGAGAGGGAGT
TTGTAAACAACTGACTACAGACATTCACATTGGGTCATCTTTAAAAAGCTGGACTCTGCT
TTTGGATGCTTCTCGGAGGCGAGTTGGATTTTGGACTGAAGTACTGTCGTTCCATTCCTT
TTTTTGAGGTGTTATGAGTGGGGCTATAACATCGCCATCCTGCGGCTTGGTGAAATTTCT
GTCTGCCCACCACTGAACAAAATATTGTCTTGCGGCATACAAACTCTTTCTGTCCACCTT
CTTACAATATACTCGAATCAACTGCTCTGCAAATTTTTCTGGCAGAAATTGTGAAACCCG
GGTTTTAATAAATCCTGATTGCTCTGTTGGGGGCAATCTTACCATAGAAGCTAACATGGT
CAAATGGATTCTTTTCTTGGTTTCCTAAATCCTGGTGGGAAACTCCACTATAAAATCTTC
AGCCTTCAGGTTTAACTTTTAGCAATACTTTGGGTTTAACCCTGGGCACCCTTTTTTGGG
AAAAAATAATAAGCGCCCCCTTTTAAAACCTTATTTGGCCGGGGGGGGGGGGGGTAAACC
CAATACTTGGATAAATTACCGGGTTCCAAATTTGGTTTAAAAAACCTCCCGGTGCGGCTT
TCTAATATGGGGTCCACA
Restriction Sites NotI-NotI |
ACCN NM_015474
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015474.2, NP_056289.2
RefSeq Size 2701 bp
RefSeq ORF 1881 bp
Locus ID 25939
UniProt ID Q9Y3Z3
Cytogenetics 20q11.23
Domains HD, SAM
Summary This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_015474" in other vectors (6)
SKU Description Size Price
RC206013 SAMHD1 (Myc-DDK-tagged)-Human SAM domain and HD domain 1 (SAMHD1) 10 ug
$583.00
RC206013L1 Lenti ORF clone of Human SAM domain and HD domain 1 (SAMHD1), Myc-DDK-tagged 10 ug
$883.00
RC206013L2 Lenti ORF clone of Human SAM domain and HD domain 1 (SAMHD1), mGFP tagged 10 ug
$883.00
RC206013L3 Lenti ORF clone of Human SAM domain and HD domain 1 (SAMHD1), Myc-DDK-tagged 10 ug
$883.00
RC206013L4 Lenti ORF clone of Human SAM domain and HD domain 1 (SAMHD1), mGFP tagged 10 ug
$883.00
RG206013 SAMHD1 (tGFP-tagged) - Human SAM domain and HD domain 1 (SAMHD1) 10 ug
$489.00 $783.00

file-alt Citations

PIAS1 potentiates the anti-EBV activity of SAMHD1 through SUMOylation
Saiada, Zhang, Li
Cell Biosci (2021) 11 (1), 127 DOI: 10.1186/s13578-021-00636-y
Cited by 18
AI Summary: OriGene’s pCMV-XL4-SAMHD1 plasmid (Cat# SC114650) was used in this study to express SAMHD1, enabling the investigation of its SUMOylation by PIAS1 and its role in restricting Epstein-Barr virus replication.
Snippet: pCMV-XL4-SAMHD1 Origene Cat# SC114650
FigureFigureFigure
PIAS1 Potentiates the Anti-viral Activity of SAMHD1 through SUMOylation
Saiada, Zhang, Li
Research Square (2021)
Snippet: pSG5-ZTA Hayward Lab Collection NA Halo-HA-BGLF4 Hayward Lab Collection pGL772 Halo-HA-UL97 Hayward Lab Collection pGL798 Halo-V5-PIAS1 Li Lab (31) pKZ28 pCMV-XL4-SAMHD1 Origene Cat# SC114650 pCMV-XL4-SAMHD1-K469R This study pSF004;- pSG5-ZTA Hayward Lab Collection NA Halo-HA-BGLF4 Hayward Lab Collection pGL772 Halo-HA-UL97 Hayward Lab Collection pGL798 Halo-V5-PIAS1 Li Lab (31) pKZ28 pCMV-XL4-SAMHD1 Origene Cat# SC114650 pCMV-XL4-SAMHD1-K469R This study pSF004
Conserved Herpesvirus Protein Kinases Target SAMHD1 to Facilitate Virus Replication
Zhang, Lv, Li
Cell Rep (2019) 28 (2), 449-459.e5 DOI: 10.1016/j.celrep.2019.04.020
AI Summary: OriGene’s human recombinant SAMHD1 protein (Cat# TP306013) was used in this study to investigate how herpesvirus protein kinases phosphorylate and inhibit SAMHD1, thereby facilitating viral replication.
Snippet: # P8010 TPA Fisher Scientific Cat# NC9325685 Sodium Butyrate (NaBu) Millipore Cat# 19137 Glutaraldehyde solution Sigma Cat# G7776 SAMHD1 human recombinant protein Origene Cat# TP306013 Critical Commercial Assays Halo-tag protein purification kit VWR/Promega Cat# PAG6790 Glutathione Sepharose 4B GE Healthcare Cat# 17–0756-01 Lipo
FigureFigureFigure
Conserved Herpesvirus Protein Kinases Target SAMHD1 to Facilitate Virus Replication
Zhang, Lv, Li
bioRxiv (2018)
Snippet: Page 1. 1 Conserved Herpesvirus Protein Kinases Target SAMHD1 to 1 Facilitate Virus Replication 2 3 4 5 6 7 Kun Zhang 1 , Dong-Wen Lv 1 and Renfeng Li 1,2,3,4* 8 9 1 Philips Institute for Oral Health Research, School of Dentistry;2 Department of Microbiology 10 View as HTML;The HA-tagged viral kinases (CHPKs) were gifts from Robert 377 Kalejta (Addgene plasmid #s 26689-26698) (Kuny et al., 2010). SAMHD1 clone (Cat# 378 SC114650) and recombinant SAMHD1 protein (Cat# TP306013) were purchased from Origene
DNA damage response signaling does not trigger redistribution of SAMHD1 to nuclear foci
Medeiros, Soares, Coelho et al
Biochem. Biophys. Res. Commun. (2018) 499 (4), 790-796 DOI: 10.1016/j.bbrc.2018.03.225
AI Summary: In this study, the human SAMHD1 plasmid (catalog number SC114650) from OriGene was used to investigate the localization and detection of SAMHD1 in cells following DNA damage.
Snippet: cells. 2. Materials and methods. Plasmids and antibodies - The plasmid encoding the human SAMHD1 construct (NM_015474) was obtained from Origene Technologies, Inc. (Rockville, MD, catalog number SC114650). Mouse
Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1
Li, Xu, Hu et al
Biochem. J. (2015)
AI Summary: OriGene contributed human SAMHD1 (SC114650) and mouse SAMHD1 (MC206493) cDNAs, which were used as templates for cloning and expression studies to investigate the interactions between SIVrcm Vpx and SAMHD1 in this paper.
Snippet: Plasmid construction 113 The human SAMHD1 and mouse SAMHD1 cDNAs were purchased from OriGene 114 (SC114650 and MC206493, respectively). These cDNAs were cloned into the 115 pcDNA3.1(+) expression vector using the BamHI and EcoRI restriction ... Cite SaveSaving
Showing 1-6 of 7 papers.
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