HOOK1 (NM_015888) Human Untagged Clone

SKU SC114574

HOOK1 (untagged)-Human hook homolog 1 (Drosophila) (HOOK1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HOOK1
• Synonyms: HK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114574 sequence for NM_015888 edited (data generated by NextGen Sequencing); ATGGAGGAGACGCAGCCGCCGCCGCAGCCTAAGCTGCCCCTGTGCGACAGCCTCATGATC; TGGCTGCAGACATTCAATACTGCCTCACCTTGTCAAGATGTCAAACAGCTGACTAGTGGA; GTTGCCATGGCACAAGTTCTTCATCAAATTGATGCAGCTTGGTTTAACGAATCTTGGTTA; AGCCGAATTAAAGAGGATGTTGGGGACAACTGGAGAATAAAGGCCAGTAATGTAAAGAAG; GTCCTTCAAGGAATTATGAGTTATTATCATGAGTTTTTGGGGCAGCAGATTTCAGAAGCA; CTTATCCCTGATTTAAACCAAATAACCGAATGTTCAGATCCAGTGGAGCTTGGGAGGTTG; CTCCAGCTTATTTTAGGTTGTGCGATCAACTGTGAAAAGAAGCAAGAACATATTCAAAAT; ATAATGACACTGGAAGAGTCTGTTCAACATGTGGTCATGACTGCTATTCAAGAGTTGATG; AGTAAAGAAATATTGAGCTCTCCTCCAAATGATGCTGTTGGAGAATTGGAGCAACAGCTT; AAAAGAGCCTTGGAAGAACTTCAGGAAGCACTAGCAGAAAAAGAAGAGCTGAGGCAAAGA; TGTGAAGAATTGGATATGCAGGTGACTACACTTCAAGATGAAAAGAATTCACTGGTTTCT; GAAAATGAGATGATGAATGAAAAACTTGACCAGTTGGATGGCTCTTTTGATGATCCAAAC; ACAGTGGTTGCAAAAAAGTATTTTCATGCACAATTACAACTAGAACAATTACAGGAAGAA; AACTTCAGGCTTGAAGCTGCAAAAGATGATTACCGTGTTCACTGTGAAGAACTTGAAAAG; CAGCTAATCGAATTCCAGCATAGGAATGATGAATTGACTAGTCTTGCAGAAGAAACAAGA; GCCCTGAAAGATGAAATAGATGTTCTTAGGGCTACCTCTGATAAAGCAAATAAACTGGAG; TCAACAGTTGAGATATATCGTCAGAAGCTACAAGATCTGAATGACCTTCGCAAGCAGGTG; AAAACTTTACAGGAAACCAACATGATGTATATGCATAATACAGTCAGCTTAGAAGAAGAA; TTAAAAAAAGCAAATGCAGCACGTACACAATTAGAAACATACAAAAGGCAGGTTCAAGAT; CTTCATGTTAAACTTTCCTCCGAATCCAAGAGGGCAGACACACTAGCGTTTGAAATGAAG; CGGCTTGAAGAAAAACATGAAGCTTTACTTAAGGAAAAAGAGAGACTAATTGAGCAGCGT; GATACTTTGAAAGAAACAAATGAAGAGCTTCGATGTTCACAAGTACAACAGGACCACCTA; AACCAAACAGATGCATCTGCTACAAAAAGTTATGAGAATCTTGCTGCTGAGATTATGCCA; GTGGAATATAGGGAGGTGTTTATTCGACTGCAACATGAAAATAAGATGCTTCGCTTACAG; CAAGAAGGCTCTGAGAATGAACGTATTGAGGAACTTCAGGAGCAGCTAGAACAGAAACAC; CGTAAAATGAATGAACTGGAAACTGAGCAGAGGCTGAGCAAAGAGCGTATTAGAGAATTG; CAGCAGCAGATTGAGGACCTCCAGAAATCTTTACAGGAACAAGGTTCCAAGTCTGAAGGC; GAAAGTTCCAGCAAATTAAAGCAGAAGTTGGAAGCTCATATGGAAAAACTCACAGAGGTC; CATGAAGAATTACAGAAGAAACAAGAACTCATTGAAGATCTTCAGCCAGATATAAATCAA; AATGTACAAAAGATCAATGAACTTGAAGCTGCTCTTCAGAAGAAAGATGAAGATATGAAA; GCAATGGAGGAAAGATATAAAATGTACTTGGAGAAAGCCAGAAATGTAATAAAAACTTTG; GATCCCAAGTTAAATCCAGCATCAGCTGAAATAATGCTACTAAGAAAGCAGTTGGCAGAG; AAAGAGAGAAGAATTGAGATTCTGGAGAGTGAATGCAAAGTAGCAAAATTCCGTGATTAT; GAAGAAAAACTCATTGTTTCTGCGTGGTATAATAAGAGTCTAGCATTCCAGAAACTGGGG; ATGGAATCTAGACTTGTGAGCGGCGGTGGTGCCTGCAGTGACACTGGTGCGTGCACTCCT; GCGCGGTCTTTCTTAGCGCAGCAACGGCACATCACCAACACCAGAAGAAATCTCTCTGTT; AAAGTCCCTGCTACAACATCTGATTAA; ; Clone variation with respect to NM_015888.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015888 unedited; CGCGAATTCGGCACGAGGCGCGTGAGCTGCGCGGGTCGTGCCTGGTACCGAGCTTTGCTG; GGGGCTAGCAGGTCGTGGACGCCGGCTCCTGGAGGAGAGCCGGTAGGAGGGAGGTGAAGG; TGTCCGCGGCGTCGTCGACAGCGGCGCCGGCCATGGAGGAGACGCAGCCGCCGCCGCAGC; CTAAGCTGCCCCTGTGCGACAGCCTCATGATCTGGCTGCAGACATTCAATACTGCCTCAC; CTTGTCAAGATGTCAAACAGCTGACTAGTGGAGTTGCCATGGCACAAGTTCTTCATCAAA; TTGATGCAGCTTGGTTTAACGAATCTTGGTTAAGCCGAATTAAAGAGGATGTTGGGGACA; ACTGGAGAATAAAGGCCAGTAATGTAAAGAAGGTCCTTCAAGGAATTATGAGTTATTATC; ATGAGTTTTTGGGGCAGCAGATTTCAGAAGCACTTATCCCTGATTTAAACCAAATAACCG; AATGTTCAGATCCAGTGGAGCTTGGGAGGTTGCTCCAGCTTATTTTAGGTTGTGCGATCA; ACTGTGAAAAGAAGCAAGAACATATTCAAAATATAATGACACTGGAAGAGTCTGTTCAAC; ATGTGGTCATGACTGCTATTCAAGAGTTGATGAGTAAAGAAATATTGAGCTCTCCTCCAA; ATGATGCTGTTGGAGAATTGGAGCAACAGCTTAAAAGAGCCTTGGAAGAACTTCAGGAAG; CACTAGCAGAAAAAGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_015888 unedited; TTCCCCCGTTAAATCTGAGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTT; TTTTATTGATAATGGACAATCATTTATTTAAAATAATATCCTAAATACTTACAAAAATAA; ATCAAATATTGGTTTTATTTTTCAAGTGTCAAAGTCTTTGCCATAGAAAATAGAAAGACC; TTTTTATAGCTAAACATTACATTTTGTGCTACATCATTTAATAAACTCCACACTCTTTTA; GGGTTNGGGCCTTACAAAATTTCCAAACTACCTCTTTACCATTGTCCAGGTGGCCCATTA; AAAAGGTTTGCCTAACCTTCACATACCCCATTTTTTCATAAGGAAAAGTTTCCCATTAAA; ACCCCCTTTTGGAAACTTAAACACGAACCCCTGGTGGGCCAAGGCGCCCTCCCACCCCCC; GGGGCCAAATTCCCCTAAAAAAAAAAGTTTGAAATGGGGAAACCTTTTTAACATTTAAAT; TACCCCCTCCTTTTTCAAAAAAAGAGCCCCCTTGGGGAAACCCCCCTTTTTTATTTTTAA; AAAAAAAAGTGCTTATTCTTCCTAAAAAATTTTGAGCCCCCCTTTTCCCCAAAAAAAAAA; CCCAATTTTTCCCCCCCCCAAAGTTTTATTCCCCCCAAAAAAAACCTGGGGCGGCTGTCC; CCCCCCTCTTTTTTTTTAAAAAAAAAGAATTTTTTTCTCTTCTAACCGCGGCTCCAACCG; AAAAACATCCTCCAAAGGTGGGCCGGGGTTCCCCCTTTAAACCCCCCGGGACATTTTTCT; TCCTTAAAAAAACACAAACCGGAAAAACCCTCCTTGGGGGATTATCTCCCCCCCCCTCTT; AACACCACAGTTGTGTAAACATCGTGTTACAAGAGAAATTGTTAATATGCCTTACCACCC; ACGGGGTGGTCAACTCCCTACATTAACCAGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_015888
• Insert Size: 6930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015888.3, NP_056972.1
• RefSeq Size: 2480 bp
• RefSeq ORF: 2187 bp
• Locus ID: 51361
• UniProt ID: Q9UJC3
• Cytogenetics: 1p32.1
• Summary: This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]