MED31 (NM_016060) Human Untagged Clone

SKU SC114523

MED31 (untagged)-Human mediator complex subunit 31 (MED31)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MED31
• Synonyms: 3110004H13Rik; CGI-125; Soh1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016060 edited; ATGGCCGCTGCTGTCGCTATGGAGACAGATGATGCTGGAAATCGACTTCGGTTTCAGTTG; GAGTTGGAATTTGTGCAATGTTTAGCCAACCCAAATTACCTTAATTTTCTTGCCCAAAGA; GGTTACTTCAAAGACAAAGCTTTTGTTAATTATCTTAAATACTTGCTTTACTGGAAAGAC; CCAGAATATGCCAAGTATCTAAAGTACCCTCAGTGTTTACACATGTTAGAGCTGCTCCAA; TATGAACACTTCCGAAAGGAGCTGGTGAATGCTCAGTGTGCGAAATTTATTGATGAACAG; CAGATTCTACATTGGCAGCACTATTCCCGGAAGCGGATGCGCCTTCAGCAAGCCTTGGCA; GAGCAGCAACAGCAAAATAACACATCGGGAAAATGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016060 unedited; CACGAGGGTGGCGTTTTGGTGTCTTCGTTTGTTATGGCCGCTGCTGTCGCTATGGAGACA; GATGATGCTGGAAATCGACTTCGGTTTCAGTTGGAGTTGGAATTTGTGCAATGTTTAGCC; AACCCAAATTACCTTAATTTTCTTGCCCAAAGAGGTTACTTCAAAGACAAAGCTTTTGTT; AATTATCTTAAATACTTGCTTTACTGGAAAGACCCAGAATATGCCAAGTATCTAAAGTAC; CCTCAGTGTTTACACATGTTAGAGCTGCTCCAATATGAACACTTCCGAAAGGAGCTGGTG; AATGCTCAGTGTGCGAAATTTATTGATGAACAGCAGATTCTACATTGGCAGCACTATTCC; CGGAAGCGGATGCGCCTTCAGCAAGCCTTGGCAGAGCAGCAACAGCAAAATAACACATCG; GGAAAATGAAAAACTGGATACAAACGAGGCACTTAATACATGTATATAATGTATTTCTTT; TGTACAGTGAAGACAAAAAAAATGAAAACTCTTCCTATCTACCTTTATTATGGTAGCCCC; TAGAACCTTTAGTGTGCTTTTTCATCCAACTTTTTATTGTTAATAGACTATTTCTGTTAA; ACCTGAATTGTTCTTTGAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC; GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016060 unedited; TTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTCAAAGAACAA; TTCAGGTTTAACAGAAATAGTCTATTAACAATAAAAAGTTGGATGAAAAAGCACACTAAA; GGTTCTAGGGGCTACCATAATAAAGGTAGATAGGAAAAGTTTTCATTTTTTTTGTCTTCA; CTGTACAAAAGAAATACATTATATACATGTATTAAGTGCCTCGTTTGTATCCAGTTTTTC; ATTTTCCCGATGTGTTATTTTGCTGTTGCTGCTCTGCCAAGGCTTGCTGAAGGCGCATCC; GCTTCCGGGAATAGTGCTGCCAATGTAAAATCTGCTGTTCATCAATAAATTTCGCACACT; GAGCATTCACCAGCTCCTTTCGGAAGTGTTCATATTGGAGCAGCTCTAACATGTGTAAAC; ACTGAGGGTACTTTAGATACTTGGCATATTCTGGGTCTTTCCAGTAAAGCAAGTATTTAA; GATAATTAACAAAAGCTTTGTCTTTGAAGTAACCTCTTTGGGCAAGAAAATTAAGGTAAT; TTGGGTTGGCTAAACATTGCACAAATTCCAACTCCAACTGAAACCGAAGTCGATTTCCAG; CATCATCTGTCTCCATAGCGACAGCAGCGGCCATAACAAACGAAGACACCAAAACGCCAC; CCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCA; CTAAACGAGCTCTGCTTATATAGACCTCCCACGTACACGCCTACCGCCCATTTGCGTCAA; CGGGGCCGGGTTATTACGACATTTTGGAAAGCCCCGTTGATTTTGTGCCAAACCAACTCC; CAGGACGTCATGGGGGGGAGACTCGTAAATCCCGTGAGTAAACGCCTATCACGCCCATTG; GGGACTGGCAAACCGCATCACCTGGTAATAGCGAGACTAATACTAAATGCCTGCCAATAG; GAAGCCCCTCAGGCATGTCTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_016060
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016060.1, NP_057144.1
• RefSeq Size: 1196 bp
• RefSeq ORF: 396 bp
• Locus ID: 51003
• UniProt ID: Q9Y3C7
• Cytogenetics: 17p13.1
• Protein Families: Transcription Factors
• Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function]