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IF3EI (EIF3L) (NM_016091) Human Untagged Clone

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SKU
SC114447
EIF3L (untagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L)
  $526.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IF3EI
Synonyms EIF3EIP; EIF3S6IP; EIF3S11; HSPC021; HSPC025; MSTP005
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114447 sequence for NM_016091 edited (data generated by NextGen Sequencing)
ATGTCTTATCCCGCTGATGATTATGAGTCTGAGGCGGCTTATGACCCCTACGCTTATCCC
AGCGACTATGATATGCACACAGGAGATCCAAAGCAGGACCTTGCTTATGAACGTCAGTAT
GAACAGCAAACCTATCAGGTGATCCCTGAGGTGATCAAAAACTTCATCCAGTATTTCCAC
AAAACTGTCTCAGATTTGATTGACCAGAAAGTGTATGAGCTACAGGCCAGTCGTGTCTCC
AGTGATGTCATTGACCAGAAGGTGTATGAGATCCAGGACATCTATGAGAACAGCTGGACC
AAGCTGACTGAAAGATTCTTCAAGAATACACCTTGGCCCGAGGCTGAAGCCATTGCTCCA
CAGGTTGGCAATGATGCTGTCTTCCTGATTTTATACAAAGAATTATACTACAGGCACATA
TATGCCAAAGTCAGTGGGGGACCTTCCTTGGAGCAGAGGTTTGAATCCTATTACAACTAC
TGCAATCTCTTCAACTACATTCTTAATGCCGATGGTCCTGCTCCCCTTGAACTACCCAAC
CAGTGGCTCTGGGATATTATCGATGAGTTCATCTACCAGTTTCAGTCATTCAGTCAGTAC
CGCTGTAAGACTGCCAAGAAGTCAGAGGAGGAGATTGACTTTCTTCGTTCCAATCCCAAA
ATCTGGAATGTTCATAGTGTCCTCAATGTCCTTCATTCCCTGGTAGACAAATCCAACATC
AACCGACAGTTGGAGGTATACACAAGCGGAGGTGACCCTGAGAGTGTGGCTGGGGAGTAT
GGGCGGCACTCCCTCTACAAAATGCTTGGTTACTTCAGCCTGGTCGGGCTTCTCCGCCTG
CACTCCCTGTTAGGAGATTACTACCAGGCCATCAAGGTGCTGGAGAACATCGAACTGAAC
AAGAAGAGTATGTATTCCCGTGTGCCAGAGTGCCAGGTCACCACATACTATTATGTTGGG
TTTGCATATTTGATGATGCGTCGTTACCAGGATGCCATCCGGGTCTTCGCCAACATCCTC
CTCTACATCCAGAGGACCAAGAGCATGTTCCAGAGGACCACGTACAAGTATGAGATGATT
AACAAGCAGAATGAGCAGATGCATGCGCTGCTGGCCATTGCCCTCACGATGTACCCCATG
CGTATTGATGAGAGCATTCACCTCCAGCTGCGGGAGAAATATGGGGACAAGATGTTGCGC
ATGCAGAAAGGTGACCCACAAGTCTATGAAGAACTTTTCAGTTACTCCTGCCCCAAGTTC
CTGTCGCCTGTAGTGCCCAACTATGATAATGTGCACCCCAACTACCACAAAGAGCCCTTC
CTGCAGCAGCTGAAGGTGTTTTCTGATGAAGTACAGCAGCAGGCCCAGCTTTCAACCATC
CGCAGCTTCCTGAAGCTCTACACCACCATGCCTGTGGCCAAGCTGGCTGGCTTCCTGGAC
CTCACAGAGCAGGAGTTCCGGATCCAGCTTCTTGTCTTCAAACACAAGATGAAGAACCTC
GTGTGGACCAGCGGTATCTCAGCCCTGGATGGTGAATTTCAGTCAGCCTCAGAGGTTGAC
TTCTACATTGATAAGGACATGATCCACATCGCGGACACCAAGGTCGCCAGGCGTTATGGG
GATTTCTTCATCCGTCAGATCCACAAATTTGAGGAGCTTAATCGAACCCTGAAGAAGATG
GGACAGAGACCTTGA

Clone variation with respect to NM_016091.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_016091 unedited
NGGGTCAATATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCAAGC
GAGGCAGCCATGTCTTATCCCGCTGATGATTATGAGTCTGAGGCGGCTTATGACCCCTAC
GCTTATCCCAGCGACTATGATATGCACACAGGAGATCCAAAGCAGGACCTTGCTTATGAA
CGTCAGTATGAACAGCAAACCTATCAGGTGATCCCTGAGGTGATCAAAAACTTCATCCAG
TATTTCCACAAAACTGTCTCAGATTTGATTGACCAGAAAGTGTATGAGCTACAGGCCAGT
CGTGTCTCCAGTGATGTCATTGACCAGAAGGTGTATGAGATCCAGGACATCTATGAGAAC
AGCTGGACCAAGCTGACTGAAAGATTCTTCAAGAATACACCTTGGCCCGAGGCTGAAGCC
ATTGCTCCACAGGTTGGCAATGATGCTGTCTTCCTGATTTTATACAAAGAATTATACTAC
AGGCACATATATGCCAAAGTCAGTGGGGGACCTTCCTTGGAGCAGAGGTTTGAATCCTAT
TACAACTACTGCAATCTCTTCAACTACATTCTTAATGCCGATGGTCCTGCTCCCCTTGAA
CTACCCAACCAGTGGCTCTGGGATATTATCGATGAGTTCATCTACCAGTTTCAGTCATTC
AGTCAGTACCGCTGTAAGACTGCCAAGAAGTCAGAGGAGGAGATTGACTNTCTTCGTTCC
AATCCCAAAATCTGGAATGTTCATAGTGTCCTCAATGTCCTTCATTCCCTGGTAGACANA
TCCAACATCAACCCGACAGTGGGAGTATACACAAGCGGNAGTGACCCTGANAGTGTGGCT
GGGGAGTATGGGNCGGACTCCCTCTACAAATGCTTGGTACCTCAGCCTGGTCGGNCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_016091 unedited
CGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCTGGCTCCAAATATCCTTTACTGAG
ATCCACTTGAAACACTTCGGTCCTTAACTTGTTAACTGAGTTGACAGGCTGATGGCTGAT
CTAGGTAAAGGTTTCACGGTAGCAAAAACACTTCCTGCCTATAATACATCAAAACAGGTT
CCTGAATGTGTGTGAATATCATCAAGGTCTCTGTCCCATCTTCTTCAGGGTTCGATTAAG
CTCCTCAAATTTGTGGATCTGACGGATGAAGAAATCCCCATAACGCCTGGCGACCTTGGT
GTCCGCGATGTGGATCATGTCCTTATCAATGTAGAAGTCAACCTCTGAGGCTGACTGAAA
TTCACCATCCAGGGCTGAGATACCGCTGGTCCACACGAGGTTCTTCATCTTGTGTTTGAA
GACAAGAAGCTGGATCCGGAACTCCTGCTCTGTGAGGTCCAGGAAGCCAGCCAGCTTGGC
CACAGGCATGGTGGTGTAGAGCTTCAGGAAGCTGCGGATGGTTGAAAGCTGGGCCTGCTG
CTGTACTTCATCAGAAAACACCTTCAGCTGCTGCAGGAAGGGCTCTTTGTGGTAGTTGGA
GTGCACATTATCATAGTTGGGCACTACAGGCGACAGGAACTTGNGGCAGGAGTAACTGAA
AAGTTCTTCATAGACTTGTGGGTCACCTTTCTGCATGCGCAACATCTTGTCCNCATATTT
CTCCAGCAGCTGGAGGTGAATGCTCTCATCNATACGCATGGNGTACATCGTGAAGGCAAT
GGNCAGCAGCGCATGCATCTGCTCATTCTGGCTGTAATCATCTCATACTTGNACGGAGGC
CTCTGGAACATGCTCTTGGTCCTCTGGATGTANAGGAAGATTGTNGCGAAAACCCGNATG
CCTCCTGNTACGACGCATCTCAATATGCAAACCCACATATATATTGGTGACCTGCACTCT
GCACACGGAATACTACTCTCTGTCATTCGAGTTTTCAA
Restriction Sites ECoRI-NOT |
ACCN NM_016091
Insert Size 1770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016091.2, NP_057175.1
RefSeq Size 1970 bp
RefSeq ORF 1695 bp
Locus ID 51386
UniProt ID Q9Y262
Cytogenetics 22q13.1
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_016091" in other vectors (5)
SKU Description Size Price
RC206690 EIF3L (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L) 10 ug
$525.00
RC206690L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit L (EIF3L), Myc-DDK-tagged 10 ug
$825.00
RC206690L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit L (EIF3L), mGFP tagged 10 ug
$825.00
RG206690 EIF3L (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit L (EIF3L) 10 ug
$725.00
SC323942 EIF3L (untagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L) 10 ug
$526.00

file-alt Citations

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