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DCTN4 (NM_016221) Human Untagged Clone

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SKU
SC114389
DCTN4 (untagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 2
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DCTN4
Synonyms DYN4; P62
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114389 sequence for NM_016221 edited (data generated by NextGen Sequencing)
ATGGCGTCCTTGCTGCAGTCGGACCGGGTTCTCTATCTAGTCCAGGGAGAAAAGAAGGTT
CGGGCCCCGCTCTCGCAACTCTACTTCTGCCGCTATTGTAGCGAACTGCGGTCGCTGGAA
TGTGTGTCTCACGAGGTGGACTCCCATTATTGTCCCAGTTGTTTAGAAAATATGCCATCG
GCTGAAGCCAAACTAAAAAAGAATAGATGTGCCAATTGTTTTGACTGTCCTGGCTGCATG
CACACCCTCTCTACTCGGGCCACGAGCATCTCCACACAGCTTCCAGATGACCCAGCCAAG
ACCACCATGAAGAAAGCCTATTACCTGGCATGTGGATTTTGTCGCTGGACGTCTAGAGAT
GTGGGCATGGCAGACAAATCTGTAGCTAGTGGCGGTTGGCAGGAACCTGAAAATCCTCAC
ACACAACGGATGAACAAATTGATTGAATATTACCAGCAGCTTGCTCAGAAAGAGAAGGTT
GAGCGAGATCGCAAGAAACTGGCACGACGTAGAAACTATATGCCTCTGGCTTTTTCGGAC
AAATATGGTCTTGGAACCAGGCTTCAGCGACCACGAGCTGGTGCATCCATCAGTACCCTT
GCCGGACTTTCCCTTAAAGAAGGAGAGGATCAGAAAGAGATAAAGATTGAGCCAGCTCAG
GCTGTGGATGAAGTGGAACCTCTACCTGAAGACTATTATACAAGACCAGTAAATTTAACA
GAGGTAACAACCCTTCAGCAGCGTCTGTTACAGCCTGACTTCCAGCCAGTCTGTGCTTCA
CAGCTCTATCCTCGCCACAAACATCTTCTGATCAAACGGTCCCTGCGCTGCCGTAAATGT
GAACATAATTTGAGCAAGCCAGAATTTAACCCAACGTCAATCAAATTCAAAATCCAGCTG
GTCGCTGTCAATTATATTCCAGAAGTGAGAATCATGTCAATTCCCAACCTTCGCTACATG
AAGGAGAGCCAGGTCCTCCTGACTCTTACAAATCCAGTTGAGAACCTCACCCATGTGACT
CTCTTCGAGTGTGAGGAGGGGGACCCTGATGATATCAACAGCACTGCTAAGGTGGTGGTG
CCTCCCAAAGAGCTCGTTTTAGCTGGCAAGGATGCAGCAGCAGAGTACGATGAGTTGGCA
GAACCTCAAGACTTTCAGGACGATCCTGACATTATAGCCTTCAGAAAGGCCAACAAAGTG
GGTATTTTCATCAAAGTTACACCACAGCGTGAGGAGGGTGAAGTGACCGTGTGCTTCAAG
ATGAAGCATGATTTTAAAAACCTGGCAGCCCCCATTCGCCCCATTGAAGAAAGTGACCAG
GGAACAGAAGTCATCTGGCTCACCCAGCATGTGGAACTTAGCTTGGGCCCACTTCTTCCT
TAA

Clone variation with respect to NM_016221.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_016221 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAAGATGGCGTCCTTG
CTGCAGTCGGACCGGGTTCTCTATTCTAGTCCAGGGAGAAAAGAAGGTTCGGGCCCCGCT
CTCGCAACTCTACTTCTGCCGCTATTGTAGCGAACTGCGGTCGCTGGAATGTGTGTCTCA
CGAGGTGGACTCCCATTATTGTCCCAGTTGTTTAGAAAATATGCCATCGGCTGAAGCCAA
ACTAAAAAAGAATAGATGTGCCAATTGTTTTGACTGTCCTGGCTGCATGCACACCCTCTC
TACTCGGGCCACGAGCATCTCCACACAGCTTCCAGATGACCCAGCCAAGACCACCATGAA
GAAAGCCTATTACCTGGCATGTGGATTTTGTCGCTGGACGTCTAGAGATGTGGGCATGGC
AGACAAATCTGTAGCTAGTGGCGGTTGGCAGGAACCTGAAAATCCTCACACACAACGGAT
GAACAAATTGATTGAATATTACCAGCAGCTTGCTCAGAAAGAGAAGGTTGAGCGAGATCG
CAAGAAACTGGCACGACGTAGAAACTATATGCCTCTGGCTTTTTCGGACAAATATGGTCT
TGGAACCAGGCTTCAGCGACCACGAGCTGGTGCATCCATCAGTACCCTTGCCGGACTTTC
CCTTAAAGAAGGAGAGGATCAGAAAGAGATAAAGATTGAGCCAGCTCAGGCTGTGGATGA
AGTGGAACCTCTACCTGAAGACTATTATACAAGACCAGTAAATNTAACAGAGGTNACCAC
CCTTCAGCAGCGTCTGTTACAGNCTGACTTNCAGCCAGTCTGTGCTTACAGCTCTTCCTC
GCACAACTCTTCTGATCAAACGTCCTGCGCTGCCGTAATGTGAACATATTGAGCAGCCGA
ATTAACCCACGTCATCAATTCAATCCACTGGGCCTGTCATATTTTCAGAGGAGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_016221 unedited
CTGCTTTATTTCACTACATTAAGTTAAGCAATAAGGCAAAACAGTAACCTGTCATTCATA
AAGATCAAGGAGTACCTCTCTGTCCCAAAGGGACCAAAAGGGTGTTTCTTCACAAATATG
CATGACACTTAGCTCATTATGCTGATAAGCACTTCAAACAAAAGAAAAGGTCAGTAGGTG
AATCAAGTAACAAATCCAAATTAACTTAGCCTAAGTTTCCAAAGCTGTAGTTTCAAGTGC
TAATGGGATCTAACTTTCCTGTGGTGGTGCATACTGACTAGCATTAAAATTTTTGCACCT
CATCTAATATAAAAAAGGCAACAAATACAATTCCTAATTCACCAGCTGCAGGTATTGAAT
CATTTCTACGAACACACAGAATACTAGCTTACACTAAAACCTATCTTGAGGAAACATTAA
ACCAGTGGCTCTATTATTCTGCAAGTTAGTTTGGCTTCTGAAGAAAAAAGTCTATGATCA
AAGTGTTCTGTGTTTCTGTGTATGGGAATGATGAGACCCATACTTCAACATTATTACTTT
GGGGATTNATACCACCTACCTGCCTAAACGCATGACATGTGTGAAGTCATGCAAACCGTG
AAACATATGAAGCTTATCTCTACTCTATGCGCCATTTCTTCACTGGCAAATATGGACCAT
GATTTTACATTAGGCTCTAGCAGGTCTGGAATAAAAACTTGAGATTCTGGGGGCTACAAA
AACCGAATTCCTTTTTTTCCTTGCAATTTGGTATTTCCCACTCCCCTGCTTCACTTCCCA
GAGGCCAACGGTTTTACCAAAGGCGTAAAAGGAATTTCTTCCTCTTACTCGGTTCAAATT
TCACTTTGTGGCGCTTTCCTAAACGTGCGCTCCCTCTGAACTTCTCCCTCTAGGTTTCCC
ACCACAATTAGGCCTTTAACCCCTCCAAACTCAAACCTCCCCGGGATTCCACGGGCTCCG
CCCTCGTCTACCTTTACGTGTTCTCATTTTCGCCGTATAAATCAGGCAATCAACCCCCGC
CTCCTACCCTTTT
Restriction Sites NotI-NotI |
ACCN NM_016221
Insert Size 3890 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016221.2, NP_057305.1
RefSeq Size 3837 bp
RefSeq ORF 1383 bp
Locus ID 51164
UniProt ID Q9UJW0
Cytogenetics 5q33.1
Protein Pathways Huntington's disease
Summary Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
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Documents
Resources
"NM_016221" in other vectors (6)
SKU Description Size Price
RC205235 DCTN4 (Myc-DDK-tagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 2 10 ug
$457.00
RC205235L1 Lenti ORF clone of Human dynactin 4 (p62) (DCTN4), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC205235L2 Lenti ORF clone of Human dynactin 4 (p62) (DCTN4), transcript variant 2, mGFP tagged 10 ug
$757.00
RC205235L3 Lenti ORF clone of Human dynactin 4 (p62) (DCTN4), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC205235L4 Lenti ORF clone of Human dynactin 4 (p62) (DCTN4), transcript variant 2, mGFP tagged 10 ug
$757.00
RG205235 DCTN4 (tGFP-tagged) - Human dynactin 4 (p62) (DCTN4), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

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