EPLIN (LIMA1) (NM_016357) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | EPLIN |
| Synonyms | EPLIN; LDLCQ8; SREBP3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_016357 |
| Insert Size | 2360 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016357.3, NP_057441.1 |
| RefSeq Size | 3664 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 51474 |
| UniProt ID | Q9UHB6 |
| Cytogenetics | 12q13.12 |
| Domains | LIM |
| Summary | This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. provided by RefSeq, Aug 2011 Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2, also known as EPLIN-beta) that is 1 aa shorter than isoform 1. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016357" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211066 | LIMA1 (Myc-DDK-tagged)-Human LIM domain and actin binding 1 (LIMA1), transcript variant b | 10 ug |
$695.00
|
|
| RC211066L1 | Lenti ORF clone of Human LIM domain and actin binding 1 (LIMA1), transcript variant b, Myc-DDK-tagged | 10 ug |
$995.00
|
|
| RC211066L2 | Lenti ORF clone of Human LIM domain and actin binding 1 (LIMA1), transcript variant b, mGFP tagged | 10 ug |
$995.00
|
|
| RC211066L3 | Lenti ORF clone of Human LIM domain and actin binding 1 (LIMA1), transcript variant b, Myc-DDK-tagged | 10 ug |
$995.00
|
|
| RC211066L4 | Lenti ORF clone of Human LIM domain and actin binding 1 (LIMA1), transcript variant b, mGFP tagged | 10 ug |
$995.00
|
|
| RG211066 | LIMA1 (tGFP-tagged) - Human LIM domain and actin binding 1 (LIMA1), transcript variant b | 10 ug |
$789.00
$895.00
|
|
Citations
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