RTF2 (NM_016407) Human Untagged Clone

SKU SC114290

RTFDC1 (untagged)-Human chromosome 20 open reading frame 43 (C20orf43)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RTF2
• Synonyms: C20orf43; CDAO5; HSPC164; RTFDC1; SHUJUN-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114290 sequence for NM_016407 edited (data generated by NextGen Sequencing); ATGGGTTGCGACGGGGGAACAATCCCCAAGAGGCATGAACTGGTGAAGGGGCCGAAGAAG; GTTGAGAAGGTCGACAAAGATGCTGAATTAGTGGCCCAATGGAACTATTGTACTCTAAGT; CAGGAAATATTAAGACGACCAATAGTTGCCTGTGAACTTGGCAGACTTTATAACAAAGAT; GCCGTCATTGAATTTCTCTTGGACAAATCTGCAGAAAAGGCTCTTGGGAAGGCAGCATCT; CACATTAAAAGCATTAAGAATGTGACAGAGCTGAAGCTTTCTGATAATCCTGCCTGGGAA; GGGGATAAAGGAAACACTAAAGGTGACAAGCACGATGACCTCCAGCGGGCGCGTTTCATC; TGCCCCGTTGTGGGCCTGGAGATGAACGGCCGACACAGGTTCTGCTTCCTTCGGTGCTGC; GGCTGTGTGTTTTCTGAGCGAGCCTTGAAAGAGATAAAAGCGGAAGTTTGCCACACGTGT; GGGGCTGCCTTCCAGGAGGATGATGTCATCGTGCTCAATGGCACCAAGGAGGATGTGGAC; GTGCTGAAGACAAGGATGGAGGAGAGAAGGCTGAGAGCGAAGCTGGAAAAGAAAACAAAG; AAACCCAAGGCAGCAGAGTCTGTTTCAAAACCAGATGTCAGTGAAGAAGCCCCAGGGCCA; TCAAAAGTTAAGACAGGGAAGCCTGAAGAAGCCAGCCTTGATTCTAGAGAGAAGAAAACC; AACTTGGCTCCCAAAAGCACAGCAATGAATGAGAGCTCTTCTGGAAAAGCTGGGAAGCCT; CCGTGTGGAGCCACAAAGAGGTCCATCGCTGACAGTGAAGAATCGGAGGCCTACAAGTCC; CTCTTTACCACTCACAGCTCCGCCAAGCGCTCCAAGGAGGAGTCTGCCCACTGGGTCACC; CACACGTCCTACTGCTTCTGA; ; Clone variation with respect to NM_016407.3; 511 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016407 unedited; NAAGGGTGCACTATTATGTAATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCT; GGCTCTGACTCCCGTCCTGCGATGGGTTGCGACGGGGGAACAATCCCCAAGAGGCATGAA; CTGGTGAAGGGGCCGAAGAAGGTTGAGAAGGTCGACAAAGATGCTGAATTAGTGGCCCAA; TGGAACTATTGTACTCTAAGTCAGGAAATATTAAGACGACCAATAGTTGCCTGTGAACTT; GGCAGACTTTATAACAAAGATGCCGTCATTGAATTTCTCTTGGACAAATCTGCAGAAAAG; GCTCTTGGGAAGGCAGCATCTCACATTAAAAGCATTAAGAATGTGACAGAGCTGAAGCTT; TCTGATAATCCTGCCTGGGAAGGGGATAAAGGAAACACTAAAGGTGACAAGCACGATGAC; CTCCAGCGGGCGCGTTTCATCTGCCCCGTTGTGGGCCTGGAGATGAACGGCCGACACAGG; TTCTGCTTCCTTCGGTGCTGCGGCTGTGTGTTTTCTGAGCGAGCCTTGAAAGAGATAAAA; GCGGAAGTTTGCCACACGTGTGGGGCTGCCTTCCAGGAGGATGATGTCATCGTGCTCAAT; GGCACCAAGGAGGATGTGGACGTGCTGAAGACAANGATGGAGGAGAGAAGGCTGAGAGCG; AAGCTGGAAAAGAATACATAGATACCCAAGGCAGCAGAGTCTGTTTCAAAACCAGATGTC; AGTGAAGAAGCCCCAGGGCCATCAAAGTTAAGACAGGGGAGCCTGTAGAACCAGCCTTGA; TTCTAAAGAGAAGAAACCAACTTGNCTCCCAAAAGCACAGCAATGAATGAGAGCTTTTTT; TGAAAAAGCTGGGAAGCCTCCGTGTTGAGCCACCAAGAGTTCATCGCTGCCATGAAGAAT; CGGAGGCCTCAAGTCCTTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016407 unedited; AGCTATGGACCCGCGGCCGCAATCTAGGATCGAGNNNTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTGGAAGAGGCAAACCAGCTTTATTTTTGAACCCAATCACCGG; GTGCCCAACTATGACTTTGCTATTTTCGAAATGATGAAATGGGGCACACTCCCCTAACAG; TATTTAGCTAGACCAACAAGGATCCTGGCTCCTCCCAGGGCTGTAACAAAGGGACGAGGC; CCTTCGAAGCTTTTAGGGGGCACAGGGTATTTGAAATCCATCCTTTTTAAAGCCCAGTTT; CACTTGGCCTACTTGATTTATTGGTGGGAGCAAGATCATTAAAATTTTAGTTAATTAATA; TGGGCCAACTCTGCACCGGGGAGGACTCTGGAGTCTCTTGTTACTGCAAGCATAAAAAGT; GCAGCCCACAGGGCTTCCTGGCCATGGAAAGGACCAAATGCACCTGCAGGTGGAAACAAG; CATGACCTCGGGCCACTGTGCAGCCCCTCCCATGTCCCCCCTGGAACCGACACGCCTCAC; ATCAAGAGTGGCCACACCAGCTTGAAGGCTGGCCAGGACAGCTTTATGACACAGAACTAT; TGAGAGAACACACAGCAGCGCACTCAGAGGCACAAAGCGACCTGCCTACGTGGAAACTAA; ACAACCTTCTGGGGCAGGAACGGTGGCAATGCGGGCTTCAAAGCAGTAGGACGTGTGGGT; GACCCAGTGGGCAGACTCCTCCTTGGAGCGCTTGGCGGAGCTGTGAGTGGTAAAGAAGGA; CTTGTAGGCCTCCGATTCTTTACTGGCAGCGATGGACCTCTTTGGGGCTCCAACGNGAGC; CTCCCAGCTTTTCAGAGAGCTCTCATCATGCTGGCTTTTGGAGCCAGATGGTTTTTCTTC; TAGAATAAGGCTGCTTCTCAAGCTTCCTGGTTAACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_016407
• Insert Size: 1610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016407.1, NP_057491.1
• RefSeq Size: 1639 bp
• RefSeq ORF: 921 bp
• Locus ID: 51507
• UniProt ID: Q9BY42
• Cytogenetics: 20q13.31
• Domains: DUF602
• Summary: Replication termination factor which is a component of the elongating replisome (Probable). Required for ATR pathway signaling upon DNA damage and has a positive activity during DNA replication. Might function to facilitate fork pausing at replication fork barriers like the rDNA. May be globally required to stimulate ATR signaling after the fork stalls or encounters a lesion (Probable). Interacts with nascent DNA (PubMed:29290612).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.