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VAMP1 (NM_016830) Human Untagged Clone

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SKU
SC114137
VAMP1 (untagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3
  $150.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VAMP1
Synonyms CMS25; SPAX1; SYB1; VAMP-1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_016830, the custom clone sequence may differ by one or more nucleotides


ATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGTCCCCCTGGCC
CTCCTCCTAACATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACAT
CATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGACCGAGCTGAT
GCCTTGCAGGCAGGAGCATCACAATTTGAGAGCAGTGCTGCCAAGCTAAAGAGGAAGTATTGGTGGAAAA
ACTGCAAGATGATGATCATGCTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGGCGGGACTG
A

5' Read Nucleotide Sequence
>OriGene 5' read for NM_016830 unedited
CATATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCCGTCCACTTC
AGCCGCAGCGTCCCTCGCCGGGTGTCTCGCCGCAGCCTCCGGAGAGGAACAGACCCTCAC
TCTCTCTGTCAGAAAAATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGAC
TGCCCCAGGTGGGGGTCCCCCTGGCCCTCCTCCTAACATGACCAGTAACAGACGACTACA
GCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACATCATACGTGTGAACGTGGACAAGGT
CCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGACCGAGCTGATGCCTTGCAGGCAGG
AGCATCACAATTTGAGAGCAGTGCTGCAAAGCTAAAGAGGAAGTATTGGTGGAAAAACTG
CAAGATGATGATCATGCTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGTAA
GTATCGCTGAAGTTGCTGGTGGGGTGAAGAGGTGGGAAGGTCCTGGAGTCTTCTCCCTGC
CTGCCTGCCTGCCTGGGGAGTGGGGCTGCTCTGACTGAGGTATGGAGACCGCTGCTATGG
GATCGTCANCCTGGTCCGGGACGGGNGGAAGGGCAGAGACAAGGGATTTCTTTGATGGAC
AAGCCTTCCCCCTGCAAAGCATTGCNAGGAGATGGCGCCTCCTGTTCTGAGGAAGTGGGG
CNTATGCTGTGTGTCACTGGGCTTGGGNTGGNANGGAGAACCATGGGGGATGGGGGGCAC
TTAACAATGGAAAGACCCCTGATGTCTGGGTACCTNGGNTGTNAATTCGCCCTCNTGGTT
TNCTNCCTGGCTCTTTCTCCTTTTTCGTCTGGGACTTNCTGATTNCCTGNNGNCCAGNNC
TACTTTTTACCTGGGAGAATGGTACACCCCCTTNNCCTGTTTGTCCATTGCCATCACCAA
TTCATGGTCCTCTGGCCTTCNGTGTTTGCTCTTAACCCACTTCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_016830 unedited
NNCCTTTTGACTTGNACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTAAAGGGGAGGAAATTTGAATTTTTTGCCTTTGTTCAAAATAC
ATGACATTGGTAAATATGCCACATGCCTTTGGGGGAAGTACAACTGTTGTTATTACTCTA
TACAAGTATGAAATCAGGGTTAGGAAAAAAAGACAAAGAGGTGATGACAGACACACAGGG
GAAACCCCACATCGTCTCATGGCAAACCGAAAAACGGGATGTGGGAAGCTCAGCTTCATT
TGACTGCAAAGTCCCAGGGTTTTGTTGCACATTTGCTCATGCACATGGGTGGGGGGAGGA
AAGGGGGATAGCAAAGACACACAGAAGAGGGTACAGGGTGGGTGAAAAAGAAAGTAAAAG
GGCTAATACCCCCAAAGAACAAGGCCAACTACACCTGGTGAGCCTCAGAGGGACAGAAAC
CCAGGAATGATTCCTGTGATAGGGCTGGGAGAGCCAAAAGGACGGATTCGCTCCAGGCTT
GGGACACATCGAGGACAGTAGGTGGTTCTTCTCCAGCGGTGACCCCCTGCATTAGGCAAG
GAGGAGCCCAGAGGAGAGTGGAGACCTTCGAGGGGGGCCGTTGGGAGGGTACTGACTGCT
TTCTTCCAGCTCTTCAATCCCGCCCTTGGGCAGAACCATAGGGAATGTGGGAAACAAGGG
CGAAGGGAAAGGAAAGATGGTTTCAGAGCCATGCAATGCTGCTGGGTGGAAAGTGGGCAT
ACAGACCCTGACCAGGATGGCCTCAGCCTTTTCCTGTTCGTGGAACCGAGGGAAAAAGGA
AAAAAGGCCCAGGGCGATTTTCCCGTTATGTCACAGCTTGCCTCCTCCCTTGGCCCGGGG
CTATCCCAAGTATAA
Restriction Sites NotI-NotI |
ACCN NM_016830
Insert Size 4360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016830.2, NP_058439.1
RefSeq Size 1104 bp
RefSeq ORF 351 bp
Locus ID 6843
UniProt ID P23763
Cytogenetics 12p13.31
Domains synaptobrevin
Protein Families Secreted Protein, Transmembrane
Protein Pathways SNARE interactions in vesicular transport
Summary Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. provided by RefSeq, Jul 2014
Transcript Variant: This variant (3), also known as VAMP-1B, contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini.
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Documents
Resources
"NM_016830" in other vectors (4)
SKU Description Size Price
RC213477 VAMP1 (Myc-DDK-tagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3 10 ug
$150.00
RC213477L3 Lenti ORF clone of Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC213477L4 Lenti ORF clone of Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3, mGFP tagged 10 ug
$450.00
RG213477 VAMP1 (tGFP-tagged) - Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3 10 ug
$489.00

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