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DIRAS2 (NM_017594) Human Untagged Clone

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SKU
SC114084
DIRAS2 (untagged)-Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2)
  $1,171.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DIRAS2
Synonyms Di-Ras2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_017594, the custom clone sequence may differ by one or more nucleotides


ATGCCTGAGCAGAGTAACGATTACCGGGTGGCCGTGTTTGGGGCTGGCGGTGTTGGCAAGAGCTCCCTGG
TGTTGAGGTTTGTGAAAGGCACATTCCGGGAGAGCTACATCCCGACGGTGGAAGACACCTACCGGCAAGT
GATCAGCTGTGACAAGAGCATATGCACATTGCAGATCACCGACACGACGGGGAGCCACCAGTTCCCGGCC
ATGCAGCGGCTGTCCATCTCCAAAGGGCACGCCTTCATCCTGGTGTACTCCATTACCAGCCGACAGTCCT
TGGAGGAGCTCAAGCCCATCTACGAACAAATCTGCGAGATCAAAGGGGACGTGGAGAGCATCCCCATCAT
GCTGGTGGGGAACAAGTGTGATGAGAGCCCCAGCCGCGAGGTGCAGAGCAGCGAGGCGGAGGCCTTGGCC
CGCACATGGAAGTGTGCCTTCATGGAGACCTCAGCCAAGCTCAACCATAACGTGAAGGAGCTTTTCCAGG
AGCTGCTCAACCTGGAGAAGCGCAGGACCGTGAGTCTCCAGATCGACGGGAAAAAGAGCAAGCAGCAGAA
AAGGAAAGAGAAGCTCAAAGGCAAGTGCGTGATCATGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_017594 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCACTCCCCCAAAATGACAC
CCACCGTCGTCAGGGTAGCATGTATAATGCCCACGTGTTAAACATTGCATTTAATCGAGA
TGCGTCCTATTGTCCTTAAGAGGGCGTTTCACACCACCAACAGTAAGCCACCCACTCTGG
AGTCACAGAATCTGCCAGGCGGTTCAAGTGAAAACCAACACACTCAGCATCCCTGGGAAC
TGAGAGGTGCCAGCAATTGCTGAAGGTGGCGATGAACACCCGAAGGTGGGAGGGAGGACT
GGTACCCACAAAGCAACATGTACCGAGAGGACTAAATGTCATCTACGTGCATGTGAGAGC
GTGTTAACCTAGAGTTACCTGCACCAACCCCAGACAGAAGCCAATCACATCTTTGGGGGA
GGGGAGGGGCAGGAAGAGGTGAGAAGATCAGATGGTCCAAAGTGGACCACACTTGGTCCA
TTTTACACTTTTTTAAAGGGGATTAAAAAACACAGCCTCTCCCCCAAAGGGTGTCCGTTC
TTAATTCCCACCTGGCCTGTTAGGAGCCTTGCTACCCTGAGGGGATGTGTTCACCTTACC
TAGACCTAGTTAGGAAGTATCATTNTAAGCTATTAGAGTATTTATCTTCATGTGCAGGGA
TAAGTGCACTAACAGTGTGCTGCTCTGTCGGAAAGTCTTCAGTTTTTAAGTGAGGATATC
GTGACAGTATTAAAACATCGCAATAATGGTCCTGTGTGTTATACATCNGAGGTTTTAGAA
TGTGATTTTCTTCTTTTGACTGTGAGGAGTATAACTTCTTCAGCCTCAGATTTAATACCA
GCAATAACTACTATTTTTAGACGTTTTTTCTNCAGGTGGTTTTCTCTCTTAATACTCGAT
CTGTACCAGCTGGGTACAGCAGCAAACCATCAAACACCAGACCATCCATTTTGTATGTCC
AA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_017594 unedited
AGAGTCGAGGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCATAACTGGAGCTTTA
TTGGAATATCTCCCATTATTTCCAGAACATCACTGGCAACTAAAAACTTGTATGCAAGGG
TGATTTTTATAACCGCAGAGTAAATAAAATAAAATAGAGAATAGACTTGTAACAATAAAT
ACACTTTAAATATAAATATACACATTGATATCCCACACATATAAAAGTCATGCTCTGCAA
ATACTTTGTATACACACTGGCCTTGTGGGGTATGAATTCCAGGATGTGTCTTCAAGGATT
TACATGTGTCCTATTAGTTGGAGATATTTCCAGAGAACTTATGTAGAAGCAACACATTAC
AAATTTTGGGGAAAAAAATTAAGTATCACAGAGCAATTTTTAAAATATTTAATACATTTT
TGTTCTACAAAGATGAGCATTTCTTAAATATTACAAACAGTGAAACAAATATACTAGCTT
ACAGATATGTACAATTTATGACTTTATACTTCAAAAATGCAGGAAGATAAATATTTATTT
TATATACATGTAATTTTAGATAGAATGAACATTCATATTGCTCTTGGGTGGCCTTGTGCA
TTGATGCATGCCCATGGCTGTCGCTGGATGGAGAAGGGCTTATGGGGATAGACGGAGTCA
TGGACCCCATGCTCATGCCAGACGCCTTCTCAAGCAAATTTAATAAAAATAACTTATCTG
GCTGGTAAAAACTGTTTGCCCTATGCAAGGTAGATGGGTTTAAGAACACAGTTCATATTT
GGGATGGAAACGTCAAATGCCTAAAAAAATTAATTTGGGAACTAACTCCCCAACTTGGAC
CATGGGGAACCCTAAAGAAAATTTGGAAACCACTGGTTCCTCAGTAGGTCATGAAAGATT
CAATGAGACCCTATGAGGGTCATAGCCTCTAGCTTAATCCAGAAAATTGAGGAAAAGGGG
CTGATGAATAACTCAGATTCAACTTTAGCGTGAACCTGAA
Restriction Sites NotI-NotI |
ACCN NM_017594
Insert Size 3380 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017594.1, NP_060064.1
RefSeq Size 4107 bp
RefSeq ORF 4107 bp
Locus ID 54769
UniProt ID Q96HU8
Cytogenetics 9q22.2
Domains RAB, ras, RAS, RHO
Summary DIRAS2 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.supplied by OMIM, Apr 2004
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Documents
Resources
"NM_017594" in other vectors (5)
SKU Description Size Price
RC200740 DIRAS2 (Myc-DDK-tagged)-Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2) 10 ug
$300.00
RC200740L3 Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2), Myc-DDK-tagged 10 ug
$600.00
RC200740L4 Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2), mGFP tagged 10 ug
$600.00
RG200740 DIRAS2 (tGFP-tagged) - Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2) 10 ug
$489.00 $500.00
SC317295 DIRAS2 (untagged)-Human DIRAS family, GTP-binding RAS-like 2 (DIRAS2) 10 ug
$330.00

file-alt Citations

RAS-related GTPases DIRAS1 and DIRAS2 induce autophagic cancer cell death and are required for autophagy in murine ovarian cancer cells
Sutton, Yang, Huang et al
Autophagy (2018)
AI Summary: In this study, OriGene products—including the anti-DIRAS2 antibody (TA809398) and pCMV-DIRAS1 and pCMV-DIRAS2 mouse and human plasmids (SC100097)—were used to detect DIRAS2 protein expression and to re-express DIRAS1 and DIRAS2 in cancer cells, enabling the investigation of their roles in autophagy and cancer cell death.
Snippet: other members of the DIRAS family. Antibody against DIRAS2 (TA 809398) was purchased from Origene. AKT (sc-514302) and MAPK/ERK (sc-514302) antibodies pCMV-DIRAS1 and pCMV- DIRAS2 mouse and human plasmids were purchased from Origene (SC100097
Showing 1-1 of 1 papers.
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