HAO1 (NM_017545) Human Untagged Clone

SKU
SC114070
HAO1 (untagged)-Human hydroxyacid oxidase (glycolate oxidase) 1 (HAO1)
$457.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HAO1
Synonyms GOX; GOX1; HAOX1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_017545 edited
GGCGGCCGCGAATTCGGCACCAGATAACCTGTGAAAATGCTCCCCCGGCTAATTTGTATC
AATGATTATGAACAACATGCTAAATCAGTACTTCCAAAGTCTATATATGACTATTACAGG
TCTGGGGCAAATGATGAAGAAACTTTGGCTGATAATATTGCAGCATTTTCCAGATGGAAG
CTGTATCCAAGGATGCTCCGGAATGTTGCTGAAACAGATCTGTCGACTTCTGTTTTAGGA
CAGAGGGTCAGCATGCCAATATGTGTGGGGGCTACGGCCATGCAGCGCATGGCTCATGTG
GACGGCGAGCTTGCCACTGTGAGAGCCTGTCAGTCCCTGGGAACGGGCATGATGTTGAGT
TCCTGGGCCACCTCCTCAATTGAAGAAGTGGCGGAAGCTGGTCCTGAGGCACTTCGTTGG
CTGCAACTGTATATCTACAAGGACCGAGAAGTCACCAAGAAGCTAGTGCGGCAGGCAGAG
AAGATGGGCTACAAGGCCATATTTGTGACAGTGGACACACCTTACCTGGGCAACCGTCTG
GATGATGTGCGTAACAGATTCAAACTGCCGCCACAACTCAGGATGAAAAATTTTGAAACC
AGTACTTTATCATTTTCTCCTGAGGAAAATTTTGGAGACGACAGTGGACTTGCTGCATAT
GTGGCTAAAGCAATAGACCCATCTATCAGCTGGGAAGATATCAAATGGCTGAGAAGACTG
ACATCATTGCCAATTGTTGCAAAGGGCATTTTGAGAGGTGATGATGCCAGGGAGGCTGTT
AAACATGGCTTGAATGGGATCTTGGTGTCGAATCATGGGGCTCGACAACTCGATGGGGTG
CCAGCCACTATTGATGTTCTGCCAGAAATTGTGGAGGCTGTGGAAGGGAAGGTGGAAGTC
TTCCTGGACGGGGGTGTGCGGAAAGGCACTGATGTTCTGAAAGCTCTGGCTCTTGGCGCC
AAGGCTGTGTTTGTGGGGAGACCAATCGTTTGGGGCTTAGCTTTCCAGGGGGAGAAAGGT
GTTCAAGATGTCCTCGAGATACTAAAGGAAGAATTCCGGTTGGCCATGGCTCTGAGTGGG
TGCCAGAATGTGAAAGTCATCGACAAGACATTGGTGAGGAAAAATCCTTTGGCCGTTTCC
AAGATCTGACAGTGCACAATATTTTCCCATCTGTATTATTTTTTTTCAGCATGTATTACT
TGACAAAGAGACACTGTGCAGAGGGTGACCACAGTCTGTAATTCCCCACTTCAATACAAA
GGGTGTCGTTCTTTTCCAACAAAATAGCAATCCCTTTTATTTCATTGCTTTTGACTTTTC
AATGGGTGTCCTAGGAACCTTTTAGAAAGAAATGGACTTTCATCCTGGAAATATATTAAC
TGTTAAAAAGAAAACATTGAAAATGTGTTTAGACAACGTCATCCCCTGGCAGGCTAAAGT
GCTGTATCCTTT
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017545 unedited
GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATAACCTGTGA
AAATGCTCCCCCGGCTAATTTGTATCAATGATTATGAACAACATGCTAAATCAGTACTTC
CAAAGTCTATATATGACTATTACAGGTCTGGGGCAAATGATGAAGAAACTTTGGCTGATA
ATATTGCAGCATTTTCCAGATGGAAGCTGTATCCAAGGATGCTCCGGAATGTTGCTGAAA
CAGATCTGTCGACTTCTGTTTTAGGACAGAGGGTCAGCATGCCAATATGTGTGGGGGCTA
CGGCCATGCAGCGCATGGCTCATGTGGACGGCGAGCTTGCCACTGTGAGAGCCTGTCAGT
CCCTGGGAACGGGCATGATGTTGAGTTCCTGGGCCACCTCCTCAATTGAAGAAGTGGCGG
AAGCTGGTCCTGAGGCACTTCGTTGGCTGCAACTGTATATCTACAAGGACCGAGAAGTCA
CCAAGAAGCTAGTGCGGCAGGCAGAGAAGATGGGCTACAAGGCCATATTTGTGACAGTGG
ACACACCTTACCTGGGCAACCGTCTGGATGATGTGCGTAACAGATTCAAACTGCCGCCAC
AACTCANGATGAAAAATTTTGAAACCAGTACTTTATCATTTTCTCCTGAGGAAAATTNTG
GAGACGACAGTGGACTTGCTGCATATGTGGCTAAAGCAATAGACCCATCTATCAGCTGGG
AAGATATCAAATGGCTGAGAAGACTGACATCATTGCCAATTGTTGCAAAGGGCATTTTGA
GAGGTGATGATGCCAGNGAGGCTGTTAAACATGGCTTTGATGGGATCTTGNTGTCGAATC
ATGGNGCTCGACNACTCGATGGGGTGCCAGCCACTATTGATGTTCTGCCAGAAATTGTGG
NAGCTGTGGAAGGGAAAGTGNAAGTCTTCTGNACNGGGTGNTGCGAAAGCACTGATGTCT
GAAGCTTTGCTCTTGCCCAAGCTGGGTTGGGGG
Restriction Sites NotI-NotI
ACCN NM_017545
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017545.2, NP_060015.1
RefSeq Size 1746 bp
RefSeq ORF 1113 bp
Locus ID 54363
UniProt ID Q9UJM8
Cytogenetics 20p12.3
Domains FMN_dh
Protein Pathways Glyoxylate and dicarboxylate metabolism, Metabolic pathways
Summary This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
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Citations

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