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DEPDC1 (NM_017779) Human Untagged Clone

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SKU
SC113941
DEPDC1 (untagged)-Human DEP domain containing 1 (DEPDC1), transcript variant 2
  $492.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DEPDC1
Synonyms DEP.8; DEPDC1-V2; DEPDC1A; SDP35
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113941 sequence for NM_017779 edited (data generated by NextGen Sequencing)
ATGGAGAGTCAGGGTGTGCCTCCCGGGCCTTATCGGGCCACCAAGCTGTGGAATGAAGTT
ACCACATCTTTTCGAGCAGGAATGCCTCTAAGAAAACACAGACAACACTTTAAAAAATAT
GGCAATTGTTTCACAGCAGGAGAAGCAGTGGATTGGCTTTATGACCTATTAAGAAATAAT
AGCAATTTTGGTCCTGAAGTTACAAGGCAACAGACTATCCAACTGTTGAGGAAATTTCTT
AAGAATCATGTAATTGAAGATATCAAAGGGAGGTGGGGATCAGAAAATGTTGATGATAAC
AACCAGCTCTTCAGATTTCCTGCAACTTCGCCACTTAAAACTCTACCACGAAGGTATCCA
GAATTGAGAAAAAACAACATAGAGAACTTTTCCAAAGATAAAGATAGCATTTTTAAATTA
CGAAACTTATCTCGTAGAACTCCTAAAAGGCATGGATTACATTTATCTCAGGAAAATGGC
GAGAAAATAAAGCATGAAATAATCAATGAAGATCAAGAAAATGCAATTGATAATAGAGAA
CTAAGCCAGGAAGATGTTGAAGAAGTTTGGAGATATGTTATTCTGATCTACCTGCAAACC
ATTTTAGGTGTGCCATCCCTAGAAGAAGTCATAAATCCAAAACAAGTAATTCCCCAATAT
ATAATGTACAACATGGCCAATACAAGTAAACGTGGAGTAGTTATACTACAAAACAAATCA
GATGACCTCCCTCACTGGGTATTATCTGCCATGAAGTGCCTAGCAAATTGGCCAAGAAGC
AATGATATGAATAATCCAACTTATGTTGGATTTGAACGAGATGTATTCAGAACAATCGCA
GATTATTTTCTAGATCTCCCTGAACCTCTACTTACTTTTGAATATTACGAATTATTTGTA
AACATTTTGGGCTTGCTGCAACCTCATTTAGAGAGGGTTGCCATCGATGCTCTACAGTTA
TGTTGTTTGTTACTTCCCCCACCAAATCGTAGAAAGCTTCAACTTTTAATGCGTATGATT
TCCCGAATGAGTCAAAATGTTGATATGCCCAAACTTCATGATGCAATGGGTACGAGGTCA
CTGATGATACATACCTTTTCTCGATGTGTGTTATGCTGTGCTGAAGAAGTGGATCTTGAT
GAGCTTCTTGCTGGAAGATTAGTTTCTTTCTTAATGGATCATCATCAGGAAATTCTTCAA
GTACCCTCTTACTTACAGACTGCAGTGGAAAAACATCTTGACTACTTAAAAAAGGGACAT
ATTGAAAATCCTGGAGATGGACTATTTGCTCCTTTGCCAACTTACTCATACTGTAAGCAG
ATTAGTGCTCAGGAGTTTGATGAGCAAAAAGTTTCTACCTCTCAAGCTGCAATTGCAGAA
CTTTTAGAAAATATTATTAAAAACAGGAGTTTACCTCTAAAGGAGAAAAGAAAAAAACTA
AAACAGTTTCAGAAGGAATATCCTTTGATATATCAGAAAAGATTTCCAACCACGGAGAGT
GAAGCAGCACTTTTTGGTGACAAACCTACAATCAAGCAACCAATGCTGATTTTAAGAAAA
CCAAAGTTCCGTAGTCTAAGATAA

Clone variation with respect to NM_017779.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017779 unedited
TATTTCCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
CGAATTCGGCACGAGGCCGAGACTCGCCACTGCCGCGGCCGCTGGGCCTGAGTGTCGCCT
TCGCCGCCGTGGACGCCACCGGGCGCTGACAGACCTATGGAGAGTCAGGGTGTGCCTCCC
GGGCCTTATCGGGCCACCAAGCTGTGGAATGAAGTTACCACATCTTTTCGAGCAGGAATG
CCTCTAAGAAAACACAGACAACACTTTAAAAAATATGGCAATTGTTTCACAGCAGGAGAA
GCAGTGGATTGGCTTTATGACCTATTAAGAAATAATAGCAATTTTGGTCCTGAAGTTACA
AGGCAACAGACTATCCAACTGTTGAGGAAATTTCTTAAGAATCATGTAATTGAAGATATC
AAAGGGAGGTGGGGATCAGAAAATGTTGATGATAACAACCAGCTCTTCAGATTTCCTGCA
ACTTCGCCACTTAAAACTCTACCACGAAGGTATCCAGAATTGAGAAAAAACAACATAGAG
AACTTTTCCAAAGATAAAGATAGCATTTTTAAATTACGAAACTTATCTCGTAGAACTCCT
AAAAGGCATGGATTACATTTATCTCCAGGAAATGGCGAGAAAATAAAGCATGACATAATC
AATGAAGATCAAGAAAATGCATTTGATTATAGAGAACTAAGCCAGGAAGATGTTGAAAGA
AGTTTGGAGATATGCTATTCTGACCTACCTGCAACCATTNTAAGTTGCCATCCCTAGAAG
AGGCATAATCCCAAACCAGTATTCCCCATTATAATGTACACATGGCCATACAAGTACGGG
GAGTAGTTTCTACAAACAAATAAAGACCTCCTACTGGGT
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_017779 unedited
NCCGGTACAGCTATGNNACCGCGGCCGCATTCTAGNGATCGGTTTTTTTTTTTTTTTTTT
GATAGCAAAAGATTTTAATCAGTAGAGATCTTAAACTCAATACAAGGCAAGAGGAAAGGA
CATAAAAATCGAGCGTGGGCTTGCTAAATTCAAGATTTAAATATATTGCCTTTCTTTCAC
TACAATCACAACTCAGTTAGGTAGATACTTTAATTCACATTTTACAGATGAGAAGATTCA
GAGTTTAAGCTTCCCAAGGACAGAAAGGTAAGTCAGTGGGAAATTCCATGTACATTCCAT
TACTAAATGCCACATAACTGTTTGGATAACATAAGAAGAGTGGGTCATTATATGATACCA
ATTAGAAGATATTAGGGATGGTGGAGGCAGTAATTTCTGGGATAAGAACTATAATTTACA
GAATAACCAGACATCATCTGATCTGGTGAAACCTGTGCATTCCCACAATTAGGCTTTTTC
ACACTTTCTCTCTTTAAATGTGCAACACCTTCCCCATCCCCTCTTTACTTGTAGCAGTTG
ATTTTGCTTCTTATATCCCGAGAAAGCAACTACCACCAAATCTACCAGTCAACTCATCTA
TATTTGAACTTAAAGATCTTTATGTTAGAATGGAATCTATCCATGTTCCAGCTTTAGCGA
AGCCCTTCTGAAATATCCATTCCTTCCTTCCTCATCAAATTTTCCCTCTTGACTAGATTA
AAAAATTTTCACCCCGTGGCATAATCCGAAACCTTTGTCTCATTAAGAAAAGATAGTTAA
TAAGGGCTCATCAATTTGCCCGGNAATTTTGGTTTGGGTTCAAGTGCTGTCCCAAGCCAC
CCGAAAGGCCACCTTTTTTGCCAAAGATCCCTTCTTGGGGCTATAACCCTAGA
Restriction Sites NotI-NotI |
ACCN NM_017779
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017779.3, NP_060249.2
RefSeq Size 3338 bp
RefSeq ORF 1584 bp
Locus ID 55635
UniProt ID Q5TB30
Cytogenetics 1p31.3
Domains DEP
Summary May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is shorter, compared to isoform a.
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Documents
Resources
"NM_017779" in other vectors (6)
SKU Description Size Price
RC209048 DEPDC1 (Myc-DDK-tagged)-Human DEP domain containing 1 (DEPDC1), transcript variant 2 10 ug
$491.00
RC209048L1 Lenti ORF clone of Human DEP domain containing 1 (DEPDC1), transcript variant 2, Myc-DDK-tagged 10 ug
$791.00
RC209048L2 Lenti ORF clone of Human DEP domain containing 1 (DEPDC1), transcript variant 2, mGFP tagged 10 ug
$791.00
RC209048L3 Lenti ORF clone of Human DEP domain containing 1 (DEPDC1), transcript variant 2, Myc-DDK-tagged 10 ug
$791.00
RC209048L4 Lenti ORF clone of Human DEP domain containing 1 (DEPDC1), transcript variant 2, mGFP tagged 10 ug
$791.00
RG209048 DEPDC1 (tGFP-tagged) - Human DEP domain containing 1 (DEPDC1), transcript variant 2 10 ug
$489.00 $691.00

file-alt Citations

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