ENOX1 (NM_017993) Human Untagged Clone

SKU SC113785

ENOX1 (untagged)-Human ecto-NOX disulfide-thiol exchanger 1 (ENOX1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ENOX1
• Synonyms: bA64J21.1; cCNOX; CNOX; PIG38
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113785 sequence for NM_017993 edited (data generated by NextGen Sequencing); ATGGTAGATGCAGGTGGAGTTGAGAACATCACCCAGCTTCCCCAGGAGCTTCCTCAGATG; ATGGCTGCAGCAGCCGATGGTTTGGGGAGTATAGCGATAGACACGACCCAGCTCAACATG; TCCGTGACAGATCCCACAGCCTGGGCTACAGCCATGAATAACCTGGGCATGGTTCCCGTA; GGGTTGCCTGGACAGCAGCTCGTGTCTGACTCAATCTGTGTCCCAGGCTTTGATCCAAGC; CTCAACATGATGACTGGAATCACCCCCATTAACCCAATGATACCAGGCCTTGGACTGGTA; CCTCCCCCACCACCAACAGAAGTGGCTGTTGTCAAAGAAATAATCCACTGCAAAAGCTGT; ACTCTTTTTCCTCAAAATCCAAATCTTCCACCTCCTTCCACAAGAGAACGACCTCCTGGG; TGTAAGACCGTGTTTGTCGGAGGATTACCAGAAAATGCTACTGAGGAAATTATTCAAGAA; GTCTTTGAACAGTGCGGTGATATTACAGCAATTCGGAAAAGCAAGAAGAATTTTTGTCAC; ATTCGCTTTGCAGAGGAATTCATGGTTGATAAAGCCATTTACCTTTCTGGTTATAGGATG; CGATTAGGGTCTAGCACCGACAAAAAGGATTCAGGCCGCCTTCATGTGGACTTTGCCCAG; GCCAGGGATGACTTCTATGAGTGGGAATGCAAGCAGAGGATGCGTGCCCGGGAGGAGCGG; CACCGGCGCAAGCTGGAGGAGGACCGGCTCAGGCCCCCATCCCCGCCTGCCATAATGCAC; TACTCGGAGCACGAAGCCGCTCTGCTGGCTGAAAAGCTGAAAGATGATAGCAAGTTTTCA; GAGGCTATCACAGTGCTGCTTTCCTGGATTGAACGAGGGGAAGTGAATCGGCGCTCTGCA; AACCAGTTCTATTCCATGGTGCAGTCGGCCAACAGCCACGTCCGCCGGCTAATGAATGAA; AAAGCCACCCATGAGCAAGAGATGGAGGAAGCCAAGGAGAATTTTAAAAATGCCTTAACT; GGGATTCTCACTCAATTTGAGCAGATTGTGGCCGTTTTCAACGCTTCTACCAGACAAAAA; GCTTGGGACCATTTCTCGAAAGCCCAGCGCAAGAACATAGACATTTGGCGAAAGCATTCT; GAGGAGCTCCGGAATGCTCAAAGTGAGCAGCTCATGGGCATCCGCCGCGAAGAAGAAATG; GAAATGTCTGATGATGAGAACTGTGACAGCCCTACAAAGAAAATGAGAGTCGATGAATCA; GCCCTGGCTGCCCAGGCCTACGCTCTGAAAGAGGAGAATGACAGTCTCCGCTGGCAGCTG; GATGCCTACAGGAATGAGGTGGAGCTGCTGAAACAAGAAAAAGAACAGCTTTTCCGAACA; GAAGAAAACCTCACCAAGGACCAGCAACTGCAGTTTCTGCAGCAAACCATGCAAGGCATG; CAGCAGCAATTGCTAACCATCCAGGAGGAGTTAAACAACAAAAAGTCAGAATTGGAACAA; GCAAAGGAAGAGCAGTCCCATACACAAGCGTTACTAAAAGTCCTGCAGGAACAATTAAAA; GGTACCAAGGAATTGGTCGAGACCAATGGCCACAGCCATGAGGATTCAAATGAAATCAAT; GTGTTGACAGTTGCATTAGTCAACCAAGACCGAGAGAACAATATTGAGAAAAGAAGCCAA; GGCTTAAAATCAGAGAAAGAAGCTCTGCTAATAGGTATCATATCAACGTTTCTTCACGTC; CATCCTTTTGGAGCCAACATAGAATATCTTTGGTCATACATGCAGCAGCTGGACTCCAAG; ATATCTGCAAATGAAATAGAAATGCTTTTGATGAGGCTGCCACGCATGTTCAAACAGGAA; TTCACGGGTGTGGGAGCCACGCTGGAAAAAAGATGGAAGTTGTGTGCCTTTGAAGGAATT; AAAACTACCTAA; ; Clone variation with respect to NM_017993.3; 1900 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017993 unedited; AATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAA; TTCGGCACGAGGCCCAGCTCCGTGCCCGGGACAGAGTCCGGGAGAAGCAAAGGAGGAGGA; GGACGCCGAGGCTAGGAGGAGCCGCGCCGCGCCCGGCGCCCCACTCCGCGCAGCGGGTTG; CCTCCGCTCCCCATACGGCGGCCCCAGGTCCTTTGCTGCCAGCATGATGTCGTATTTATA; ATATGTGGAGGAGAGAGAGCTCACCATGCTCAGAAGCCTCTGGTATGTCAGAACCATGCT; GTCTTCCATGAGACTTCCTTTGTGAAGAGCATCCATTTAAAAGACTTTTATGAATACATG; GTTTCAATCAAGTCCCCAGAGAACACATTTGTCTTCTGAGCTGCTGGCAGTTTTGAGAAT; CTGATGACCTCCGAGGGGACCCTGCACTCAGCCATCAAAGTGTTCCTGCCCCTCTGGACA; CTCATAATTCAATGGTAGATGCAGGTGGAGTTGAGAACATCACCCAGCTTCCCCAGGAGC; TTCCTCAGATGATGGCTGCAGCAGCCGATGGTTTGGGGAGTATAGCGATAGACACGACCC; AGCTCAACATGTCCGTGACAGATCCCACAGCCTGGGCTACAGCCATGAATAACCTGGGCA; TGGTTCCCGTAGGGTTGCCTGGACAGCAGCTCGTGTCTGACTCAATCTGTGTCCCCAGCT; TTGATCCAAGCCTCACATGATGACTGGAATCACCCCCATTAACCCAATGATACCAGGCCT; GGNACTGGTACCCTNCCCCACCCCAACAGAAGTGGGCTGTTGTCAAAGAAATATCCACTG; CNAAAGCTGTACTCTTTTTCCTCAAATCCAAATCTCCACCTCCTTNCACAGAAACGACTC; CTGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017993 unedited; GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTACTAAAACA; GAAAGACTTTCAGGGAAAATGTCATTTAATGATTTCAGCTATACATTACATATACAACTC; TGTAGCCTAGCAACAATCATTGCTACACCATCATTAACTTTAAATATTTTGCTTGGTGAG; CTATTCTCAAATGTAGCACATTTTAAGATTTTGCCATCATTATGACTGTGGAATCATTGT; TTGGTTTTCATAGGAAACAGATCTGTCACAGTATGAAGCTTTGTTTATTTTAAGAAACTG; GTACACAATTACATTTCCACTTACAAGAGAACGCCACAGATATAACTAAAGGCAGGCTTC; GATGGCTCCACAAAGGTTGCGTGCGGGACCAACCCCACACCTCCTGCTTCCCCGTCGCAC; CGCCCTGGCCAGGTTCACATGGTTTCATTTCCAGAGATGCTTTGCTCTTCGCAGTTAGGT; AGTTTTAATTCCTTCAAAGGCACACAACTTCCATCTTTTTTCCAGCGTGGCTCCCACACC; CGTGAATTCCTGTTTGAACATGCGTGGCAGCCTCATCAAAAGCATTTCTATTTCATTTGC; AGATATCTTGGAGTCCAGCTGCTGCATGTATGACCAAAGATATTCTATGTTGGCTCCAAA; AGGATGGACGTGAAGAAACGTTGATATGATACCTATTACCAGAGCTTCTTTCTCTGATTT; TAAGCCTTGGCTTCTTTTCTCAATATTGGTCTCTCGGTCTTGGGTTGACTAATGCCACTG; TCCACACCATTGATTCCTTTGAATCCTCATGCCTGGGGCCATTGTGCCTCGACCATTCCT; TGGACCTTTTAATGGTCCCTGCAGGACTTTTATTACCCCTGGGTTATGGAACCGTTTTCC; TT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017993
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017993.1, NP_060463.1
• RefSeq Size: 2624 bp
• RefSeq ORF: 1932 bp
• Locus ID: 55068
• UniProt ID: Q8TC92
• Cytogenetics: 13q14.11
• Domains: RRM
• Summary: The protein encoded by this gene is involved in plasma membrane electron transport pathways. The encoded protein has both a hydroquinone (NADH) oxidase activity and a protein disulfide-thiol interchange activity. The two activities cycle with a periodicity of 24 minutes, with one activity being at its peak when the other is at its lowest. [provided by RefSeq, Dec 2016]; Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 10. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1-9 all encode the same isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.