RNMTL1 (MRM3) (NM_018146) Human Untagged Clone

SKU
SC113706
RNMTL1 (untagged)-Human RNA methyltransferase like 1 (RNMTL1)
  $457.00
4 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RNMTL1
Synonyms RMTL1; RNMTL1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_018146, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGCTGGTGAGACCCGCGAGGTTTGTCGTGCGACCGTTGCTGCAGGTGGTCCAGGCTTGGGACC
TTGACGCGAGGCGCTGGGTCCGGGCGCTGCGGCGGAGCCCAGTGAAAGTGGTGTTTCCTTCCGGAGAGGT
GGTGGAACAGAAGCGCGCTCCTGGGAAGCAGCCCCGCAAGGCACCATCTGAGGCCAGTGCCCAGGAGCAA
CGAGAGAAACAACCGCTCGAGGAGTCCGCATCCCGCGCTCCCAGCACCTGGGAAGAGTCTGGGCTTCGCT
ACGATAAAGCTTATCCCGGGGACAGGAGGCTGAGCAGTGTAATGACAATAGTAAAGTCCAGGCCATTTCG
GGAAAAACAAGGGAAGATCCTGCTGGAAGGTCGCAGGCTCATTTCAGACGCTCTCAAGGCTGGAGCTGTG
CCAAAAATGTTCTTCTTTAGCCGTCTAGAATACCTAAAGGAGTTGCCAGTCGATAAGCTGAAAGGTGTCA
GCCTCATTAAGGTGAAATTTGAGGATATCAAGGATTGGTCCGACCTCGTAACGCCACAAGGAATAATGGG
GATTTTTGCCAAGCCTGACCATGTTAAGATGACATATCCAAAGACTCAGCTTCAGCATTCACTGCCTTTA
TTATTGATTTGTGACAATCTCCGTGACCCTGGGAACCTGGGGACAATTCTGAGATCTGCAGCTGGGGCAG
GCTGCAGCAAAGTGTTACTCACCAAAGGCTGTGTGGATGCCTGGGAGCCCAAAGTGCTCCGGGCGGGTAT
GGGCGCACATTTCCGGATGCCCATTATCAATAATCTGGAATGGGAAACCGTGCCCAATTACCTGCCCCCT
GACACTCGGGTCTATGTGGCTGACAACTGTGGCCTTTATGCCCAGGCTGAGATGTCTAATAAAGCTAGTG
ACCATGGCTGGGTGTGTGATCAACGAGTGATGAAGTTTCACAAGTATGAGGAAGAGGAAGATGTAGAAAC
CGGAGCCAGTCAAGATTGGCTGCCTCATGTTGAGGTTCAGAGTTACGACTCGGACTGGACAGAGGCGCCG
GCAGCTGTGGTGATTGGCGGGGAGACCTACGGCGTGAGCCTGGAGTCCCTGCAGCTGGCCGAGAGCACTG
GTGGCAAGAGGCTGCTGATCCCCGTTGTGCCTGGTGTGGACAGCCTCAACTCGGCCATGGCGGCAAGCAT
CCTGCTTTTCGAAGGGAAAAGACAGCTGCGGGGGAGGGCGGAGGACTTGAGCAGGGACAGGAGTTACCAC
TGA


5' Read Nucleotide Sequence
>OriGene 5' read for NM_018146 unedited
GTATCCGACTTCTGATAGGCGGCCGCGAATTCGCACGAGCTCAGGAACATGGCGGCGCTG
GTGAGACCCGCGAGGTTTGTCGTGCGACCGTTGCTGCAGGTGGTCCAGGCTTGGGACCTT
GACGCGAGGCGCTGGGTCCGGGCGCTGCGGCGGAGCCCAGTGAAAGTGGTGTTTCCTTCC
GAAGAGGTGGTGGAACAGAAGCGCGCTCCTGGGAAGCAGCCCCGCAAGGCACCATCTGAG
GCCAGTGCCCAGGAGCAACGAGAGAAACAACCGCTCGAGGAGTCCGCATCCCGCGCTCCC
AGCACCTGGGAAGAGTCTGGGCTTCGCTACGATAAAGCTTATCCCGGGGACAGGAGGCTG
AGCAGTGTAATGACAATAGTAAAGTCCAGGCCATTTCGGGAAAAACAAGGGAAGATCCTG
CTGGAAGGTCGCAGGCTCATTTCAGACGCTCTCAAGGCTGGAGCTGTGCCAAAAATGTTC
TTCTTTAGCCGTCTAGAATACCTAAAGGAGTTGCCAGTCGATAAGCTGAAAGGTGTCAGC
CTCATTAAGGTGAAATTTGAGGATATCAAGGATTGGTCCGACCTCGTAACGCCACAAGGA
ATAATGGGGATTTTTGCCAAGCCTGACCATGTTAAGATGACATATCCAAAGACTCAGCTT
CAGCATTCACTGCCTTTATTATTGATTTGTGACCATCTCCGTGACCCTGGGAACCTGGNG
GACATTCTGAGATCTGCAGCTGGGGCAGGCTGCANCAAAGTGTTACTCACCAAAGCTGTG
TGGATGCCTGGGAGCCCAAATGCTCCCGGCGGGTATGGCCGCCATTTTCGGATGCCATAT
CATAATCTGGATGGAAACCGTCGCCATTCTGCCCCTGAACTGGGTCTTGTGGCTGACACT
GTGC
Restriction Sites NotI-NotI |
ACCN NM_018146
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018146.2, NP_060616.1
RefSeq Size 1815 bp
RefSeq ORF 1263 bp
Locus ID 55178
UniProt ID Q9HC36
Cytogenetics 17p13.3
Domains SpoU_methylase
Protein Families Stem cell - Pluripotency
Summary Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2015
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_018146" in other vectors (4)
SKU Description Size Price
RC204113 RNMTL1 (Myc-DDK-tagged)-Human RNA methyltransferase like 1 (RNMTL1) 10 ug
$457.00
RC204113L3 Lenti ORF clone of Human RNA methyltransferase like 1 (RNMTL1), Myc-DDK-tagged 10 ug
$757.00
RC204113L4 Lenti ORF clone of Human RNA methyltransferase like 1 (RNMTL1), mGFP tagged 10 ug
$757.00
RG204113 RNMTL1 (tGFP-tagged) - Human RNA methyltransferase like 1 (RNMTL1) 10 ug
$489.00 $657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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