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DARS2 (NM_018122) Human Untagged Clone

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SKU
SC113685
DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein
  $971.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DARS2
Synonyms ASPRS; LBSL; MT-ASPRS; mtAspRS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_018122, the custom clone sequence may differ by one or more nucleotides


ATGTACTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAAC
CGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTT
TGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA
TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCA
TTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCA
AGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAA
ATCAAAGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGA
AGAAAACAGAGGCTCTTCGGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCT
GCGACTGAGGTCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA
GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCCAGGGAACCTG
GAAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGCGGTTTAGACAG
ATATTTTCAGGTTGCCCGATGTTATCGAGATGAAGGTTCAAGACCAGACAGACAGCCTGAGTTTACTCAG
ATTGACATAGAGATGTCATTTGTAGACCAGACTGGGATCCAGAGTTTAATTGAGGGTTTGCTCCAGTATT
CCTGGCCCAATGACAAAGATCCTGTGGTTGTTCCTTTTCCTACTATGACTTTTGCTGAGGTGCTGGCCAC
CTATGGAACTGATAAACCTGACACTCGCTTTGGAATGAAGATTATAGATATCAGTGATGTGTTTAGAAAC
ACAGAGATTGGATTTCTTCAAGATGCACTTAGTAAGCCCCATGGAACTGTGAAAGCCATATGTATCCCTG
AAGGAGCAAAATACTTAAAAAGGAAAGACATTGAATCCATTAGAAACTTTGCAGCTGACCATTTTAATCA
GGAAATCTTACCTGTATTCCTTAACGCCAATAGAAACTGGAATTCTCCAGTTGCTAATTTCATAATGGAG
TCACAAAGACTGGAATTAATCAGACTAATGGAGACCCAAGAGGAAGATGTGGTCCTACTAACTGCTGGAG
AGCACAATAAAGCATGCTCTTTGTTAGGAAAATTACGACTGGAATGTGCTGACCTTCTAGAAACAAGAGG
AGTGGTGCTCCGTGACCCCACTCTGTTCTCTTTCCTTTGGGTGGTAGATTTCCCACTCTTCCTGCCCAAG
GAGGAAAATCCCAGAGAGCTGGAATCGGCCCACCACCCATTTACTGCTCCCCACCCCAGTGACATACATC
TCCTGTACACTGAGCCCAAAAAGGCCCGTAGCCAACACTATGACTTGGTTTTAAATGGCAATGAAATAGG
AGGTGGTTCAATTCGAATTCACAATGCAGAGCTGCAGCGTTATATCCTGGCAACCTTACTAAAGGAGGAT
GTGAAAATGCTCTCCCATCTGCTCCAGGCTTTAGATTATGGGGCACCCCCTCATGGAGGAATTGCCTTAG
GGTTAGACAGACTGATATGCCTTGTCACTGGATCTCCAAGCATCAGAGATGTCATAGCCTTCCCAAAGTC
CTTCCGGGGACATGACCTCATGAGCAATACCCCAGATTCTGTCCCTCCTGAGGAACTGAAGCCCTATCAT
ATCCGAGTCTCCAAGCCAACAGACTCCAAAGCAGAAAGAGCTCATTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_018122 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCTCTCTAC
AGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTTTGTTGTCCGG
ACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA
TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTT
CAAGTTATCATTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCC
CCTGTGGAATCTGTGGTGCAAGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAG
AATCCAAAAATGCCAACAGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTTCTGAAT
GCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGAAGAAAACAGAGGCTCTTCGG
TTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCTGCGACTGAGG
TCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA
GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCC
AGGGAACCTGGNAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTG
ATGGTTGGCGGGTTAGACAGATATTTTCAAGTTGCCCGATGTTATCGAGATGAAGTTTCA
AGACCAGACAGACAGCCCTNAGTTTACTCAGATTGACATAGAGATGTCATTTGTAGACCA
GACTGGGATCCAGAGTTTATATGAGGGTTTGCTCCAGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018122 unedited
GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATAATTATTAGTTTATTAAAAC
TTTTATGTGTATGATTAGAACTTAAAATCACAATGAGTTTTGGTGTTCACTTTTTCGCAC
ATTTGAGGTTAATCTAAGGTTGATTAGAATTTTCAATTACTTATAATTGAGAGAAAATAG
TGTTCAGTGATTAATAATATCTTTGTTGTCTTTTTTTTTTTTGAGATGGAGTCTTGCTCT
GTCACCCAGGCTGGAGTGTAATGGCACCATCTTGGCTCACTGCAACCTCCATCTCCCAGG
TTCAAGCAAACCTCATGCCTCAGCCTCCCGAGTGGCTGCGATTACAGGCATGCGCCACCA
TGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACTATGTTGGCCAGGCTGGTG
TTGAACTCCTGACCTCAAGTGATCCACCCACCTCAATCTCCCAAAGTGCTGGGATTACAG
GTGTGAGCCACCACTCCCGGCCAATAATATCTTTAAAACTAAAAAAAAAACAGAAAATTG
ATAAGTAAAATGACTTTTACTATTGATATCAGTTNTGTTTAATCTGAAACTATAAAATAG
AGATATATCTACCTATTTATGGATGGATAATACCATCATCTCATATTTGGGGAATGCTTT
ATAGTTTTTCAAAGTACTTTTATTCACTTTATCTTCCATATATGGGATCCTCTCTGATTC
CAGCAATAACAGCCCGGTGAGGTAGCCAGGGCAAGTATGTTTTTTACACTTTAACAGGAA
GGGAAGCTAAGCGAGGTTTATGTACTTACTCAGCCTGAAACACTGAAGAAAAATTGTGAC
TCTCATTTCAGGGAGGTTTCTGCTTATTAAAAATTTAGCCCCTCCTCCTTATATTGGGGG
GGTGGAAGGCCTTTTAACCTAATTTAATGATCCTCTGCGAGGGGAGAAACATGGTCCTTC
CTCCTATTTACGGG
Restriction Sites NotI-NotI |
ACCN NM_018122
Insert Size 3370 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018122.1, NP_060592.1
RefSeq Size 3348 bp
RefSeq ORF 3348 bp
Locus ID 55157
UniProt ID Q6PI48
Cytogenetics 1q25.1
Domains GAD, tRNA-synt_2
Protein Pathways Aminoacyl-tRNA biosynthesis
Summary The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). provided by RefSeq, Nov 2009
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Documents
Resources
"NM_018122" in other vectors (7)
SKU Description Size Price
RC207360 DARS2 (Myc-DDK-tagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein 10 ug
$600.00
RC207360L1 Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$900.00
RC207360L2 Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$900.00
RC207360L3 Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$900.00
RC207360L4 Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$900.00
RG207360 DARS2 (tGFP-tagged) - Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein 10 ug
$489.00 $800.00
SC318102 DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein 10 ug
$661.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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