CISD1 (NM_018464) Human Untagged Clone

SKU
SC113478
CISD1 (untagged)-Human CDGSH iron sulfur domain 1 (CISD1)
$150.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CISD1
Synonyms C10orf70; MDS029; mitoNEET; ZCD1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_018464, the custom clone sequence may differ by one or more nucleotides


ATGAGTCTGACTTCCAGTTCCAGCGTACGAGTTGAATGGATCGCAGCAGTTACCATTGCTGCTGGGACAG
CTGCAATTGGTTATCTAGCTTACAAAAGATTTTATGTTAAAGATCATCGAAATAAAGCTATGATAAACCT
TCACATCCAGAAAGACAACCCCAAGATAGTACATGCTTTTGACATGGAGGATTTGGGAGATAAAGCTGTG
TACTGCCGTTGTTGGAGGTCCAAAAAGTTCCCATTCTGTGATGGGGCTCACACAAAACATAACGAAGAGA
CTGGAGACAATGTGGGCCCTCTGATCATCAAGAAAAAAGAAACTTAA


5' Read Nucleotide Sequence
>OriGene 5' read for NM_018464 unedited
NTTCAGATNTGAAAACGATTACTATAGGCGGCCGCGAAATTCGCACGAGGCTGCGCGGTA
TCATCGCGGAGTCGGTGCTTTAGTACGCCGCTGGCACCTTTACTCTCGCCGGCCGCGCGA
ACCCGTTTGAGCTCGGTATCCTAGTGCACACGCCTTGCAAGCGACGGCGCCATGAGTCTG
ACTTCCACCTCCAGCGCACGAGTTGAATGGATCGCAGCAGACACCATTGCTGCTGGGACA
GCTGCAATTGGTTATCTAGCTTACAAAAGATTTTATGTTACAAGATCATCCGAAATAAAG
CTATGATAAACCTTCACATCCAGAAAGACAACCCCAAGATAGTACATGCTTTTGACATGG
AGGATTTGGGAGATAACAGCTGCGTACCTGCCGTTGCTGGAGGTCCAAAAAGTTCCCATT
CTGTGATGGGGCTCACACAAAACATAACGAAGAGACTGGAGACAATGTGGGCCCTCTGAT
CATCAAGAAAAAAGAAACTTACATGGACACTTCTGATGCTGCAAATCAGCTTGTCGTGAA
GTTACCTGCATGCTTAATTACAATGACTACCACCTCTGTCTGATTCACCTTCGCTGGATC
CTAAATGTGGTATATTGCAAACTGCAGCTCTCACATTCATGGCATTTGTCTTGTTGAAAC
ATCGCGGTGCCCACTTGTTTACACACAAAAAAAAAAAAAAACCTCGACTTTTAATTGCGG
GCGGGGTCTTACCTCGTCTCTGACCAGATCCCGGTGGCATCCCTGTGACCCCTCCCAAGC
CCTTCTCTGCCCTGGAAGTGCCCCTCCATGGCCACCACCCTGCACAATAACATAACAGGC
TCATTTGTCTGATAAGGGCCCTCTATAAATTTGGGGCGGGCGGGCCGCTTTCACCCGGCC
CAACT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018464 unedited
GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTAAACAAATGTGCACCACGATGT
TTCAACAAGACAAATGCCATAAATGTGAAAGCTGCAGTTTGCAATATACCACATTTAGAA
TCCAGCGAAGGTGAATCAGACAGAGGTGGTAGTCATTCTAATTAAACAATCAGGTAACTT
CACGACAAGCTGATTTGCAGCATCAAAAGTGTCCATTTAAGTTTCTTTTTTCTTGATGAT
CAGAGGGCCCACATTGTCTCCAGTCTCTTCGTTATGTTTTGTGTGAGCCCCATCACAGAA
TGGGAACTTTTTGGACCTCCAACAACGGCAGTACACAGCTTTATCTCCCAAATCCTCCAT
GTCAAAAGCATGTACTATCTTGGGGTTGTCTTTCTGGATGTGAAGGTTTATCATAGCTTT
ATTTCGATGATCTTTAACATAAAATCTTTTGTAAGCTAGATAACCAATTGCAGCTGTCCC
AGCAGCAATGGTAACTGCTGCGATCCATTCAACTCGTACGCTGGAACTGGAAGTCAGACT
CATGGCGCCGTCGCTTGCAAGGCGTGTGCACTAGGATACCGAGCTCAAACGGGTTCGCGC
GGCCGGCGAGAGTAAAGGTGCCAGCGGCGTACTAAAGCACCGACTCCGCGATGCTACCGC
GCAGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGG
TTCACTAAACGAGCTCTGCTTATATAGACCCTTCACCGTACACGCCTACCGCCCATTTGC
GTCAACGGGCGGGGGNTATACGACATTNTGGAAAGTCCCGTGATTTTGTGCCCAAACAAC
TCCATTGACGTCATGGGGNTGGAGACTTGNAANTCCNGTGAGTCAAACCGTATCCACGCC
ATGGTGTACTGGCAAACCCGCTACCATGGTATACCGAGACTAAACCTAAATGACTGCCAG
NTAGAAAGCCNGTAGGTCATGACTGGCATAATGCAGCGGCCATTAC
Restriction Sites NotI-NotI
ACCN NM_018464
Insert Size 590 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018464.2, NP_060934.1
RefSeq Size 925 bp
RefSeq ORF 327 bp
Locus ID 55847
UniProt ID Q9NZ45
Cytogenetics 10q21.1
Domains ZnF_CDGSH
Protein Families Transmembrane
Summary This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.