ERMAP (NM_018538) Human Untagged Clone

SKU SC113423

ERMAP (untagged)-Human erythroblast membrane-associated protein (Scianna blood group) (ERMAP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ERMAP
• Synonyms: BTN5; PRO2801; RD; SC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018538, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGATGGCGAGTTCTGCTGGCTCCTGGCTCTCTGGCTGCCTCATCCCTCTCGTCTTCCTCCGGCTGT; CTGTGCATGTGTCAGGCCACGCAGGGGATGCCGGCAAGTTCCACGTGGCCCTACTAGGGGGCACAGCCGA; GCTGCTCTGCCCTCTCTCCCTCTGGCCCGGGACGGTACCCAAGGAGGTGAGGTGGCTGCGGTCCCCATTC; CCGCAGCGCTCCCAGGCTGTTCACATATTCCGGGATGGGAAGGACCAGGATGAAGATCTGATGCCGGAAT; ATAAGGGGAGGACGGTGCTAGTGAGAGATGCCCAAGAGGGAAGTGTCACTCTGCAGATCCTTGACGTGCG; CCTTGAGGACCAAGGGTCTTACCGATGTCTGATCCAAGTTGGAAATCTGAGTAAAGAGGACACCGTGATC; CTGCAGGTTGCAGCCCCATCTGTGGGGAGTCTCTCCCCCTCAGCAGTGGCTCTGGCTGTGATCCTGCCTG; TCCTGGTACTTCTCATCATGGTGTGCCTTTGCCTTATCTGGAAGCAAAGAAGAGCAAAAGAAAAGCTTCT; CTATGAACATGTGACGGAGGTGGACAATCTTCTTTCAGACCATGCTAAAGAAAAAGGAAAACTCCATAAA; GCTGTCAAGAAACTCCGGAGTGAACTGAAGTTGAAAAGAGCTGCAGCAAACTCAGGCTGGAGAAGAGCCC; GGTTGCATTTTGTGGCAGTGACCCTGGACCCAGACACAGCACATCCCAAACTCATCCTTTCTGAGGACCA; AAGATGTGTAAGGCTTGGAGACAGACGGCAGCCTGTACCTGACAACCCCCAGAGATTTGATTTCGTTGTC; AGCATCCTAGGCTCTGAGTACTTCACGACTGGCTGCCACTACTGGGAGGTGTATGTGGGAGACAAGACCA; AATGGATTCTTGGAGTATGTAGTGAGTCAGTGAGCAGGAAGGGGAAGGTTACTGCCTCACCTGCCAATGG; ACACTGGCTTCTGCGACAGAGTCGTGGGAATGAGTATGAAGCTCTCACATCCCCGCAGACCTCCTTCCGC; CTTAAAGAGCCTCCACGGTGTGTGGGGATTTTCCTGGACTATGAAGCAGGAGTCATCTCTTTCTACAATG; TGACCAACAAGTCCCACATCTTTACTTTCACCCACAATTTCTCTGGCCCCCTTCGCCCTTTCTTTGAACC; TTGCCTTCATGATGGAGGAAAAAACACAGCACCTCTAGTCATTTGTTCAGAACTACACAAATCAGAGGAA; TCAATTGTCCCCAGGCCAGAAGGGAAAGGCCATGCTAATGGAGATGTGTCCCTCAAGGTGAACTCTTCTT; TACTACCCCCGAAGGCCCCAGAGCTGAAGGATATAATCCTGTCCTTGCCCCCTGACCTTGGCCCAGCCCT; TCAGGAGCTCAAGGCTCCTTCTTTTTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018538 unedited; GTTGTTCGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGCAGC; TCTCTAAAAGCAAGCATGTTTTAGACCACTCACTCTTTCCCTCTTTCTTCAGGAATGAAT; TGGGAAAGGCTGATGAGTAAAACATACCATCCTTTTCTATTTTCTTGATGCTGTTTACAA; CATAGTTTGGTGATATCCAGAGCTAATGTACATGCTTTCAAAAGCTAATCTGCCTGTTGA; TGGTAACTAGGTACAGCGACTTTAAATACAGTTGCTATAATCCTGAAAAGCCCCAGGAGC; ACATCAGGGAGCTGGGAAACACAGTTGCAGAGAACTCAGCTGTATGTTGCCCTCTGACCT; TGGCCCAGACCTTCAGAGGCTCAGTCTGTTGAGTCTGTTGTGACTGTCTTATGAATCAAT; CTGTTGTGCCAACCCTTTCTGAGATTCAGAGAGGTCCAGCTAGAAAAAGTGGCAGTTTTA; ACCACCAACGTAGAAGCTTTTTTTTCCCCACAAGACCATTGTACTGCAATACTACATTTT; TTGTGGANNNANANAGNCTCTTAAGTTGGAGAGTATTGATTTCAAATAGGAAGTTGGTAG; ACTAGGTGCGAAGATGAAAAAATGACCCTTGATTTTGGGCGGGCTTTGCAGAACCCCCTC; CCCTTTTCAGTCACATTATAATTGCCAACCTGGCTTAACCTGGGAAAACTTTGTTTAACA; GTTTGCAAAAGAAAACCCCGTTTTTATCCAACAAAAACTGTTTCTTTTTTGTTTGGCAAA; AAGGTCCTCCGCTTATTCCCGTTTTAAACCAAGTTTTCCCAAAACAAACAAACAATTTAT; ATTGGGGCGCGGGGGGCGGGGCTTTTAAAGAACTCCCCCCCGGTTTATGGGCGCGGTAAA; CCCCAAAATGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018538 unedited; CTATGGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTCAACAAATACCAAACTTTTTATTTTAAAACTGTACAAAAGCAAACAAAA; AATTCCACTGAGTCACTGGGATAGTTAATCTTATGTATCCACTTAACTGGGTCACAAAAT; GCCCAGCTTTTTGGTCAAACATTATTCTGAGTGTTCCTGGGAGGGGGTTTCTCAATGACT; TTAACATTCAAATCAGTAAACTGAGTAATGCAAACTGTCCTCCCAAATGGGGGCGGCCCT; TGCTGAATCAGTTGAAGGCCTGAATAAAACAAAAAAGCTAACTCTGCCCTAAAAAAATTC; TTCCTGCCTAATAGCCTTGGGACAGGACCATCAGCTTTTTCCTTGCCTTCGGACTTGAAT; TAAACATCATCTCTTCCTTGGCGTAAAGCCTGCTGCCATCAAACTGGAACTAAACCATCA; GCTTTCATGGGACTCCATCTTGCTGACTTATCCTGCAGATCTTGGAATTTAACCGCCTCC; ATAATCCCGTGAGCACTTCCCTTAAAATAACCACCGCCCCCCTGCCCCATAATATATGGA; TCTTGTTCCCTTGGAAAACCTTGACTAATCCCGGACCTACCGGAGATTTTTTGCACAACA; AAAAAATCAGTTTCTGTGGGAAAAAACGGGTATCCCTCCGCAACTGGAACCCCCTTCCCC; CTGGGTAGGCCCCATCCCTATTTGTCGGCCCTGATACCAAAAGGCCTTCGCATTCACCTC; AAAACAGGGCGCTTATTCCTCCCCCCCCTTCCTCCAATCCCTTTTGAAAGCATCCTCCAT; TTGGCGCATATATCCCCGNCGCCCGTGCTGTTTTTGCCCCTCGCCCCCTTTCTTCGTTAA; TCCCGCCCCCCCCCCCCGAGCCTTGTCCCGCGCCCCCGCGGGACCGCTCGGTGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_018538
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018538.1, NP_061008.1
• RefSeq Size: 3381 bp
• RefSeq ORF: 3381 bp
• Locus ID: 114625
• UniProt ID: Q96PL5
• Cytogenetics: 1p34.2
• Domains: IG, IGv, PRY, SPRY
• Protein Families: Druggable Genome, Transmembrane
• Summary: The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor, possibly as a mediator of cell adhesion. Polymorphisms in this gene are responsible for the Scianna/Radin blood group system. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) has an alternate 5' UTR and encodes the same protein, as compared to variant 1. Sequence Note: This RefSeq record represents the ERMAP*001.1.1 allele.