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WSB2 (NM_018639) Human Untagged Clone

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SKU
SC113401
WSB2 (untagged)-Human WD repeat and SOCS box containing 2 (WSB2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WSB2
Synonyms SBA2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113401 sequence for NM_018639 edited (data generated by NextGen Sequencing)
ATGGAGGCCGGAGAGGAACCGCTGCTGCTGGCCGAACTCAAGCCCGGGCGCCCCCACCAG
TTTGATTGGAAGTCCAGCTGTGAAACCTGGAGCGTCGCCTTCTCCCCAGATGGCTCCTGG
TTTGCTTGGTCTCAAGGACACTGCATCGTCAAACTGATCCCCTGGCCGTTGGAGGAGCAG
TTCATCCCTAAAGGGTTTGAAGCCAAAAGCCGAAGTAGCAAAAATGAGACGAAAGGGCGG
GGCAGCCCAAAAGAGAAGACGCTGGACTGTGGTCAGATTGTCTGGGGGCTGGCCTTCAGC
CCGTGGCCTTCCCCACCCAGCAGGAAGCTCTGGGCACGCCACCACCCCCAAGTGCCCGAT
GTCTCTTGCCTGGTTCTTGCTACGGGACTCAACGATGGGCAGATCAAGATCTGGGAGGTG
CAGACAGGGCTCCTGCTTTTGAATCTTTCCGGCCACCAAGATGTCGTGAGAGATCTGAGC
TTCACACCCAGTGGCAGTTTGATTTTGGTCTCCGCGTCACGGGATAAGACTCTTCGCATC
TGGGACCTGAATAAACACGGTAAACAGATTCAAGTGTTATCGGGCCACCTGCAGTGGGTT
TACTGCTGTTCCATCTCCCCAGACTGCAGCATGCTGTGCTCTGCAGCTGGAGAGAAGTCG
GTCTTTCTATGGAGCATGAGGTCCTACACGTTAATTCGGAAGCTAGAGGGCCATCAAAGC
AGTGTTGTCTCTTGTGACTTCTCCCCCGACTCTGCCCTGCTTGTCACGGCTTCTTACGAT
ACCAATGTGATTATGTGGGACCCCTACACCGGCGAAAGGCTGAGGTCACTCCACCACACC
CAGGTTGACCCCGCCATGGATGACAGTGACGTCCACATTAGCTCACTGAGATCTGTGTGC
TTCTCTCCAGAAGGCTTGTACCTTGCCACGGTGGCAGATGACAGACTCCTCAGGATCTGG
GCCCTGGAACTGAAAACTCCCATTGCATTTGCTCCTATGACCAATGGGCTTTGCTGCACA
TTTTTTCCACATGGTGGAGTCATTGCCACAGGGACAAGAGATGGCCACGTCCAGTTCTGG
ACAGCTCCTAGGGTCCTGTCCTCACTGAAGCACTTATGCCGGAAAGCCCTTCGAAGTTTC
CTAACAACTTACCAAGTCCTAGCACTGCCAATCCCCAAGAAAATGAAAGAGTTCCTCACA
TACAGGACTTTTTAA

Clone variation with respect to NM_018639.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018639 unedited
TGTCACATATTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCTGCCG
CGGGGGCCGCTCGCGTCCTCCTGGAGGCCGGAGAGGAACCGCTGCTGCTGGCCGAACTCA
AGCCCGGGCGCCCCCACCAGTTTGATTGGAAGTCCAGCTGTGAAACCTGGAGCGTCGCCT
TCTCCCCAGATGGCTCCTGGTTTGCTTGGTCTCAAGGACACTGCATCGTCAAACTGATCC
CCTGGCCGTTGGAGGAGCAGTTCATCCCTAAAGGGTTTGAAGCCAAAAGCCGAAGTAGCA
AAAATGAGACGAAAGGGCGGGGCAGCCCAAAAGAGAAGACGCTGGACTGTGGTCAGATTG
TCTGGGGGCTGGCCTTCAGCCCGTGGCCTTCCCCACCCAGCAGGAAGCTCTGGGCACGCC
ACCACCCCCAAGTGCCCGATGTCTCTTGCCTGGTTCTTGCTACGGGACTCAACGATGGGC
AGATCAAGATCTGGGAGGTGCAGACAGGGCTCCTGCTTTTGAATCTTTCCGGCCACCAAG
ATGTCGTGAGAGATCTGAGCTTCACACCCAGTGGCAGTTTGATTTTGGTCTCCGCGTCAC
GGGATAAGACTCTTCGCATCTGGGACCTGAATAAACACGGTAAACAGATTCAAGTGTTAT
CGGGCCACCTGCAGTGGGTTTACTGCTGTTCCATCTCCCCAGACTGCAGCATGCTGTGCT
CTGCAGCTGGAGAGAAGCCGTCTTTCTATGGAGCATGAAGTCCCTACACGTTATTCCGAA
GCTAGAGGGCCATCAAAGCAGTGTTGCCTCTTGGGACTCCTCCCCCGACTTTGCCCTGCT
TGTCACGGCTTCTTACGATACCATGTGAATATGTGGGACCCCTACACGCGCGAAAGGCTG
AGGTCCCTCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018639 unedited
TGGCAACTTTCCAGGGCCAGGNAGNAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTCGTACATAAGAGTATCAGCTAAAGTCATGACTACCCCAAT
TCTTTACACAATTAAAACATCTTTGCAACTGTTCAATTTAAGTACTATGGTTTTTAAATA
ATCGAGAAAAACACAGTTGTACCTTAACATCTGTTGAGAAAATACAAATAAATATGATGC
TAATAAAGGGCCACTGATAACTCAGTAGCCATCTGAATAGTCATGCGGTTTAAAAATACA
TCCTTGTATAATCTGACATACAAATTTGTCATTTCCTGCACATGCACACCATTGTTAAAN
NNACAAAAAAAAAAGCCAGTAATAGGGTCTGGATCGGTCAGGAGCACGGCCTCTGAGTCC
CCTGTAATTTAGTTAAGCTAAATTAATACCTCATACCAAAGGGCTCCAGGAAAACTGTCC
TGCAGGTCAAAAGGGAGCCCAAAAAAAAAAGCATTTGGCCAACATCGAAGCAACTTTGAC
CACAGCAGGAGAGAACTTGAACCAATGCTACCACTGCATCAGAAACACAAGGATAGGTAC
TAGGCAAAAGCCATCGTTCCCAGCGAGGGGAGTGTGAGCTGCTCTGCCACTGAGAGGCTC
TGGAGGCCTACGCCGCNNNNAGCCAAACAACTCAGTAACTGGACTTTGAATCACAACAGC
AGAAAGAGTTAACACTGGCTGTTCATACTGGACTGANATTTACGTAGGGCTTTGTTGCCG
GCAAGGGAATTCTTTCTTTAAACTGACTTTCCTTGCCGGAGAAAAAATCCTGGCTAGACC
GGGAACGGGTTGGCACATCCTTCCCC
Restriction Sites ECoRI-NOT |
ACCN NM_018639
Insert Size 2140 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018639.3, NP_061109.1
RefSeq Size 2833 bp
RefSeq ORF 1215 bp
Locus ID 55884
UniProt ID Q9NYS7
Cytogenetics 12q24.23
Domains SOCS, WD40
Protein Families Druggable Genome
Summary This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2013
Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
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"NM_018639" in other vectors (4)
SKU Description Size Price
RC202375 WSB2 (Myc-DDK-tagged)-Human WD repeat and SOCS box containing 2 (WSB2) 10 ug
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RC202375L3 Lenti ORF clone of Human WD repeat and SOCS box containing 2 (WSB2), Myc-DDK-tagged 10 ug
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RC202375L4 Lenti ORF clone of Human WD repeat and SOCS box containing 2 (WSB2), mGFP tagged 10 ug
$757.00
RG202375 WSB2 (tGFP-tagged) - Human WD repeat and SOCS box containing 2 (WSB2) 10 ug
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