DAZAP1 (NM_018959) Human Untagged Clone

SKU SC113312

DAZAP1 (untagged)-Human DAZ associated protein 1 (DAZAP1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DAZAP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018959, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAG; AGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCAC; CAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTGCTGGCCAGC; AGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGA; GAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGT; CGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG; GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACG; AACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACG; AGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTT; GTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAG; GAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCC; GCCACCCCCACCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC; CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGT; TTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCC; GTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCA; GGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACG; GGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCA; CAACGTGCAAGGGTTCCACCCCTACCGACGCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018959 unedited; NGTTAGATTTTGTATACGACTTCTATAGGGCGGCCGCGAATTCGCACGAGGAGGGGAGGA; GGCAGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGTTGCGCAGAT; CCGGGCCGCGGCTGTGGGGAGGGCGACGGAGCGGGTGACCTTCCGGAGGCGGGAGCGAGC; GAGGAGGCCCGGGAGCGCCGAGCGTCGCCGCCGCCGCCGCCATGAACAACTCGGGCGCCG; ACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAGAGACTCTGC; GCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCA; CCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGG; TGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACAC; CCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGA; GCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATTCCTCACAATTGTGGTGAGA; CAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATG; ACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAG; TGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAAC; GAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGG; GGAGCCCGGNTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGC; AGCAAGGATATGGCCCCGCAGGAATGTGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_018959
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018959.2, NP_061832.2
• RefSeq Size: 2185 bp
• RefSeq ORF: 1224 bp
• Locus ID: 26528
• UniProt ID: Q96EP5
• Cytogenetics: 19p13.3
• Domains: RRM
• Protein Families: Stem cell - Pluripotency
• Summary: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform b, which is longer and has a distinct C-terminus, compared to isoform a.