TREM2 (NM_018965) Human Untagged Clone

SKU SC113273

TREM2 (untagged)-Human triggering receptor expressed on myeloid cells 2 (TREM2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TREM2
• Synonyms: PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC113273 representing NM_018965.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCGGCCGCGAATTCGGCACGAGGCTGACATGCCTGATCCTCTCTTTTCTGCAGTTCAAGGGAAAGACGA; GATCTTGCACAAGGCACTCTGCTTCTGCCCTTGGCTGGGGAAGGGTGGCATGGAGCCTCTCCGGCTGCT; CATCTTACTCTTTGTCACAGAGCTGTCCGGAGCCCACAACACCACAGTGTTCCAGGGCGTGGCGGGCCA; GTCCCTGCAGGTGTCTTGCCCCTATGACTCCATGAAGCACTGGGGGAGGCGCAAGGCCTGGTGCCGCCA; GCTGGGAGAGAAGGGCCCATGCCAGCGTGTGGTCAGCACGCACAACTTGTGGCTGCTGTCCTTCCTGAG; GAGGTGGAATGGGAGCACAGCCATCACAGACGATACCCTGGGTGGCACTCTCACCATTACGCTGCGGAA; TCTACAACCCCATGATGCGGGTCTCTACCAGTGCCAGAGCCTCCATGGCAGTGAGGCTGACACCCTCAG; GAAGGTCCTGGTGGAGGTGCTGGCAGACCCCCTGGATCACCGGGATGCTGGAGATCTCTGGTTCCCCGG; GGAGTCTGAGAGCTTCGAGGATGCCCATGTGGAGCACAGCATCTCCAGGAGCCTCTTGGAAGGAGAAAT; CCCCTTCCCACCCACTTCCATCCTTCTCCTCCTGGCCTGCATCTTTCTCATCAAGATTCTAGCAGCCAG; CGCCCTCTGGGCTGCAGCCTGGCATGGACAGAAGCCAGGGACACATCCACCCAGTGAACTGGACTGTGG; CCATGACCCAGGGTATCAGCTCCAAACTCTGCCAGGGCTGAGAGACACGTGAAGGAAGATGATGGGAGG; AAAAGCCCAGGAGAAGTCCCACCAGGGACCAGCCCAGCCTGCATACTTGCCACTTGGCCACCAGGACTC; CTTGTTCTGCTCTGGCAAGAGACTACTCTGCCTGAACACTGCTTCTCCTGGACCCTGGAAGCAGGGACT; GGTTGAGGGAGTGGGGAGGTGGTAAGAACACCTGACAACTTCTGAATATTGGACATTTTAAACACTTAC; AAATAAATCCAAGACTGTCATATTTAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018965 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGACATGCCTGAT; CCTCTCTTTTCTGCAGTTCAAGGGAAAGACGAGATCTTGCACAAGGCACTCTGCTTCTGC; CCTTGGCTGGGGAAGGGTGGCATGGAGCCTCTCCGGCTGCTCATCTTACTCTTTGTCACA; GAGCTGTCCGGAGCCCACAACACCACAGTGTTCCAGGGCGTGGCGGGCCAGTCCCTGCAG; GTGTCTTGCCCCTATGACTCCATGAAGCACTGGGGGAGGCGCAAGGCCTGGTGCCGCCAG; CTGGGAGAGAAGGGCCCATGCCAGCGTGTGGTCAGCACGCACAACTTGTGGCTGCTGTCC; TTCCTGAGGAGGTGGAATGGGAGCACAGCCATCACAGACGATACCCTGGGTGGCACTCTC; ACCATTACGCTGCGGAATCTACAACCCCATGATGCGGGTCTCTACCAGTGCCAGAGCCTC; CATGGCAGTGAGGCTGACACCCTCAGGAAGGTCCTGGTGGAGGTGCTGGCAGACCCCCTG; GATCACCGGGATGCTGGAGATCTCTGGTTCCCCGGGGAGTCTGAGAGCTTCGAGGATGCC; CATGTGGAGCACAGNNTNTCCAGGAGCCTCTTGGAAGGAGAAATCCCCTTCCCACCCACT; TCCATCCTTCTCCTCCTGGCCTGCATCTTTCTCATCAAGATTCTAGCAGCCAGCGCCCTC; TGGGCTGCAGCCCTGCATGGACAGAAAGCCAGGACACATCCACCCAGTGACCTGGACTGT; GGCCATGACCCCAAGTATCAGCTCCAAACTCTGCCANGGCTGAGAGACACGTGAAGGAAA; AGATGGGAGGAAAAGCCCAGNAGAATTCCACCAGGGACANNCCAGCCTGCATACTTTGCA; CTTGGCACCAGACCTTCTGTCTGCTCTGCAGAACTCTTGCCTGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018965 unedited; GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATATGACAGTCT; TGGATTTATTTGTAAGTGTTTAAAATGTCCAATATTCAGAAGTTGTCAGGTGTTCTTACC; ACCTCCCCACTCCCTCAACCAGTCCCTGCTTCCAGGGTCCAGGAGAAGCAGTGTTCAGGC; AGAGTAGTCTCTTGCCAGAGCAGAACAAGGAGTCCTGGTGGCCAAGTGGCAAGTATGCAG; GCTGGGCTGGTCCCTGGTGGGACTTCTCCTGGGCTTTTCCTCCCATCATCTTCCTTCACG; TGTCTCTCAGCCCTGGCAGAGTTTGGAGCTGATACCCTGGGTCATGGCCACAGTCCAGTT; CACTGGGTGGATGTGTCCCTGGCTTCTGTCCATGCCAGGCTGCAGCCCAGAGGGCGCTGG; CTGCTAGAATCTTGATGAGAAAGATGCAGGCCAGGAGGAGAAGGATGGAAGTGGGTGGGA; AGGGGATTTCTCCTTCCAAGAGGCTCCTGGAGATGCTGTGCTCCACATGGGCATCCTCGA; AGCTCTCAGACTCCCCGGGGAACCAGAGATCTCCAGCATCCCGGTGATCCAGGGGGTCTG; CCAGCACCTCCACCAGGACCTTCCTGAGGGTGTCAGCCTCACTGCCATGGAGGCTCTGGC; ACTGGTAGAGACCCCGCATCATGGGGTTGTAGATTCCGCAGCGTAATGGTGAGAGTGCCA; CCCCAGGTATCGTCTGTGATGGCTGTGCTCCCATTCCACCTCCTCAGAAGACAGCAGCCA; CAAGTTGTGCGTGCTGACCACCCGCTGGCATGGGCCCCTTCTCTCCCACCTGCGGGAACC; AGCCCTTGCGCCCCCCCCAGTGCTTTATGGNAGTCATAGGGCCAAGACACCTGCAAGAAC; TTGGCCCCNCCCGCCTGAACACTGGGTAGTGGTGGCCTCCGACACTTCTGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_018965
• Insert Size: 1099 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018965.1
• RefSeq Size: 1041 bp
• RefSeq ORF: 1099 bp
• Locus ID: 54209
• UniProt ID: Q9NZC2
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 39.4 kDa
• Summary: This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).