EIF2B3 (NM_020365) Human Untagged Clone

SKU SC113103

EIF2B3 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EIF2B3
• Synonyms: EIF-2B; EIF2Bgamma
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113103 sequence for NM_020365 edited (data generated by NextGen Sequencing); ATGGAATTTCAAGCAGTAGTGATGGCAGTAGGTGGAGGATCTCGGATGACAGACCTAACT; TCCAGCATTCCCAAACCTCTGCTTCCAGTTGGGAACAAACCTTTAATTTGGTACCCATTG; AACCTGCTTGAGCGTGTTGGATTTGAAGAAGTCATTGTGGTTACAACCAGGGATGTTCAA; AAGGCTCTATGTGCAGAATTCAAGATGAAAATGAAGCCAGATATTGTGTGTATTCCTGAT; GATGCTGACATGGGAACTGCAGATTCTTTGCGCTACATATATCCAAAACTTAAGACAGAT; GTGCTGGTGCTGAGCTGTGATCTGATAACAGACGTTGCCTTACATGAGGTTGTGGACCTG; TTTAGAGCTTATGATGCATCACTTGCTATGTTGATGAGAAAAGGCCAAGATAGCATAGAA; CCTGTTCCCGGTCAAAAGGGGAAAAAAAAAGCAGTGGAGCAGCGTGACTTCATTGGAGTG; GACAGCACAGGAAAGAGGCTGCTCTTCATGGCTAATGAAGCAGACTTGGATGAAGAGCTG; GTCATTAAGGGATCCATCCTACAGAAGCATCCTAGAATACGTTTCCACACGGGTCTTGTG; GATGCCCACCTCTACTGTTTGAAAAAATACATCGTGGATTTCCTAATGGAAAATGGGTCA; ATAACTTCTATCCGGAGTGAACTGATTCCATATTTAGTGAGAAAACAGTTTTCCTCAGCT; TCCTCACAACAGGGACAAGAAGAAAAAGAGGAGGATCTAAAGAAAAAGGAGCTGAAGTCC; TTAGATATCTACAGTTTTATAAAAGAAGCCAATACACTGAACCTGGCTCCCTATGATGCC; TGCTGGAATGCCTGTCGAGGAGACAGGTGGGAAGACTTGTCCAGATCACAGGTGCGCTGC; TATGTCCACATCATGAAAGAGGGGCTCTGCTCTCGAGTGAGCACACTGGGACTCTACATG; GAAGCAAACAGACAGGTGCCCAAATTGCTGTCTGCTCTCTGTCCAGAAGAACCACCAGTC; CATTCGTCAGCCCAGATTGTCAGCAAACACCTGGTTGGAGTTGACAGCCTCATTGGGCCA; GAGACACAGATTGGAGAGAAGTCATCCATTAAGCGCTCAGTCATTGGCTCATCCTGTCTC; ATAAAAGATAGAGTGACTATTACCAATTGCCTTCTCATGAACTCAGTCACTGTGGAGGAA; GGAAGCAATATCCAAGGCAGTGTCATCTGCAACAATGCTGTGATCGAGAAGGGTGCAGAC; ATCAAGGACTGCTTGATTGGAAGTGGCCAGAGGATTGAAGCCAAAGCTAAACGAGTGAAT; GAGGTGATCGTGGGGAATGACCAGCTCATGGAGATCTGA; ; Clone variation with respect to NM_020365.3; 243 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020365 unedited; CACATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGCGTGCGGGG; AGCTGAGTTATAGCTGTGACTTCTGCCCTGCCAGGCCGCACACAAGCTGGCTGACCCGGT; TTGTAAAAATGGAATTTCAAGCAGTAGTGATGGCAGTAGGTGGAGGATCTCGGATGACAG; ACCTAACTTCCAGCATTCCCAAACCTCTGCTTCCAGTTGGGAACAAACCTTTAATTTGGT; ACCCATTGAACCTGCTTGAGCGTGTTGGATTTGAAGAAGTCATTGTGGTTACAACCAGGG; ATGTTCAAAAGGCTCTATGTGCAGAATTCAAGATGAAAATGAAGCCAGATATTGTGTGTA; TTCCTGATGATGCTGACATGGGAACTGCAGATTCTTTGCGCTACATATATCCAAAACTTA; AGACAGATGTGCTGGTGCTGAGCTGTGATCTGATAACAGACGTTGCCTTACATGAGGTTG; TGGACCTGTTTAGAGCTTATGATGCATCACTTGCTATGTTGATGAGAAAAGGCCAAGATA; GCATAGAACCTGTTCCCGGTCAAAAGGGGAAAAAAAAAGCAGTGGAGCAGCGTGACTTCA; TTGGAGTGGACAGCACAGGAAAGAGGCTGCTCTTCATGGCTAATGAAGCAGACTTGGATG; AAGAGCTGGTCATTAAGGGATCCATCCTACAGAAGCATCCTAGAATACGTTTCCACACGG; GNTCTTGTGGATGCCCACCTCTACTGTTNGAAAAATACATCGTGGATTTCCTAATGGAAA; ATGGGNNTCATACTTCTATCCNNGAGTGACTGNATNNCATATTAGTGANGAAACAGTTTT; TCTCAGCTTCCCCTCACACAGGGACCAGAAGAAAAGAGNAGGATCTAANGAAAAGGAGNN; CTGAGTCNTAGTATCTACANTTTTATAAAGAAGCCATACACTGAACTGGNCTCCTATGAT; GCTGCTGNAATGCTGTCNAGGAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020365 unedited; GATCGCTGCCGCAATTTANATCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTACCCCTGGGACCCCACCTGACATGGGACCTTTGGACTGGTC; CACAAGTCTCCAGCATGCCTTTGGAAGCCCTTTTTTATTGGGAAATAAATACCGAGTTAA; ACAGGGGGGCCGGCCAACATCTGGGGCTTTTTAGGCCAAAAGGAAGGAGTCTGACTTGGT; CAAAACTCAAATCTCCATGAGCTGGTCATTCCCCACGATCACCTCATTCACTCGTTTAGC; TTTGGCTTCAATCCTTTGGCCACTTCCAATCAAGCAGTCCTTGATGTCTGCCCCCTTTTC; GATCACAGCATTGTTGCAAATGACACTGCCTTGGATATTGCTTCCTTCCTCCCAGGGACT; GAGTTCATGAAAAGGCAATTGGGAATAGTCACTCTATCTTTTATGAAACAGGATGAGCCA; ATGACTGAGCGCTTAATGGATGACTTCTCTCCAATCTGTGTCTCTGGCCCAATGAGGCTG; TCAACTCCAACCAGGTGTTTGCTGACAATCTGGGCTGACCAATGGACTGGTGGTTCTTCT; GGACAGAAAGCACACAGCAATTTGGGCACCTGTCTGTTTTGCTCCATGTAGAGTCCCCAG; TGGGCTCACTCGAAAGCAGAGCCCCTTTTCATGATGTGGACATAGCAGCGCACCTGTGAT; CTGGACAGATTTTCCCACCTGTTTTCTCGACAGGGATTCCAGCAGGCATTATAAGGAGCC; AGGTTCCAGGGATTGCCTTCTTTTTAAAACTGGGAAATCTTAGGACTCCGCTCCTTTTTC; TTTAAACCTCCCCTTTTTTTTTTGCCCCCTGTGGGAGAAACTTGAGAAACTGTTTTCCCC; TAAATATGGAACAGTTCCTCCGGAAGAAATTTTGCCCCTTTCCCTTAGAAACCCCCAGTT; TTTTTCAACAGAGAGGGGCCCTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_020365
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020365.1, NP_065098.1
• RefSeq Size: 1602 bp
• RefSeq ORF: 1359 bp
• Locus ID: 8891
• UniProt ID: Q9NR50
• Cytogenetics: 1p34.1
• Domains: NTP_transferase
• Summary: The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) encodes the longer isoform (1).