SLAMF7 (NM_021181) Human Untagged Clone

SKU SC113001

SLAMF7 (untagged)-Human SLAM family member 7 (SLAMF7)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLAMF7
• Synonyms: 19A; CD319; CRACC; CS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113001 sequence for NM_021181 edited (data generated by NextGen Sequencing); ATGGCTGGTTCCCCAACATGCCTCACCCTCATCTATATCCTTTGGCAGCTCACAGGGTCA; GCAGCCTCTGGACCCGTGAAAGAGCTGGTCGGTTCCGTTGGTGGGGCCGTGACTTTCCCC; CTGAAGTCCAAAGTAAAGCAAGTTGACTCTATTGTCTGGACCTTCAACACAACCCCTCTT; GTCACCATACAGCCAGAAGGGGGCACTATCATAGTGACCCAAAATCGTAATAGGGAGAGA; GTAGACTTCCCAGATGGAGGCTACTCCCTGAAGCTCAGCAAACTGAAGAAGAATGACTCA; GGGATCTACTATGTGGGGATATACAGCTCATCACTCCAGCAGCCCTCCACCCAGGAGTAC; GTGCTGCATGTCTACGAGCACCTGTCAAAGCCTAAAGTCACCATGGGTCTGCAGAGCAAT; AAGAATGGCACCTGTGTGACCAATCTGACATGCTGCATGGAACATGGGGAAGAGGATGTG; ATTTATACCTGGAAGGCCCTGGGGCAAGCAGCCAATGAGTCCCATAATGGGTCCATCCTC; CCCATCTCCTGGAGATGGGGAGAAAGTGATATGACCTTCATCTGCGTTGCCAGGAACCCT; GTCAGCAGAAACTTCTCAAGCCCCATCCTTGCCAGGAAGCTCTGTGAAGGTGCTGCTGAT; GACCCAGATTCCTCCATGGTCCTCCTGTGTCTCCTGTTGGTGCCCCTCCTGCTCAGTCTC; TTTGTACTGGGGCTATTTCTTTGGTTTCTGAAGAGAGAGAGACAAGAAGAGTACATTGAA; GAGAAGAAGAGAGTGGACATTTGTCGGGAAACTCCTAACATATGCCCCCATTCTGGAGAG; AACACAGAGTACGACACAATCCCTCACACTAATAGAACAATCCTAAAGGAAGATCCAGCA; AATACGGTTTACTCCACTGTGGAAATACCGAAAAAGATGGAAAATCCCCACTCACTGCTC; ACGATGCCAGACACACCAAGGCTATTTGCCTATGAGAATGTTATCTAG; ; Clone variation with respect to NM_021181.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021181 unedited; NCCGTTCACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGNAN; AAAGCAATATGGCTGGTTCCCCAACATGCCTCACCCTCATCTATATCCTTTGGCAGCTCA; CAGGGTCAGCAGCCTCTGGACCCGTGAAAGAGCTGGTCGGTTCCGTTGGTGGGGCCGTGA; CTTTCCCCCTGAAGTCCAAAGTAAAGCAAGTTGACTCTATTGTCTGGACCTTCAACACAA; CCCCTCTTGTCACCATACAGCCAGAAGGGGGCACTATCATAGTGACCCAAAATCGTAATA; GGGAGAGAGTAGACTTCCCAGATGGAGGCTACTCCCTGAAGCTCAGCAAACTGAAGAAGA; ATGACTCAGGGATCTACTATGTGGGGATATACAGCTCATCACTCCAGCAGCCCTCCACCC; AGGAGTACGTGCTGCATGTCTACGAGCACCTGTCAAAGCCTAAAGTCACCATGGGTCTGC; AGAGCAATAAGAATGGCACCTGTGTGACCAATCTGACATGCTGCATGGAACATGGGGAAG; AGGATGTGATTTATACCTGGAAGGCCCTGGGGCAAGCAGCCAATGAGTCCCATAATGGGT; CCATCCTCCCCATCTCCTGGAGATGGGGAGAAAGTGATATGACCTTCATCTGCGTTGCCA; GGAACCCTGTCAGCAGAAACTTCTCAAGCCCCATCCTTGCCAGGAAGCTCTGTGAAGGTG; CTGCTGATGACCCAGATTCCTCCATGGTCCTCCTGTGTCTCCTGTTGGTGCCCCTCCTGC; TCAGTCTCTTTGTACTGGGGCTATTTCTTTGGTTTCTGAAGAGAGAGAGACAAGAAGAGT; ACATTGNAGAGAAGAAGAGAGTGGACATTTGTCGGNAAACTNNCTACATATGCCCCCATT; CTGAGAGAACACAGATACGACACAATCCCTCAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021181 unedited; GGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTATGGGAATCTCACAATA; TCTGTTTTATTAAGATAGTAAAGAAGACTTTTTTACTACAATAGGGTTTTGCAGTAGGGA; GAGAGATCTGGCTCAATTCCTTCACAATGCCTTTCTGAGAGAAGATAATCCTCAATCCCA; TTCTTGCCCAACAAGCATTTGTTATTAAGTTTTTCAACACTTTCATCACTTGGTAGGGAG; GTCTCCATTCAAGATATGTGTCAGGGAACATATCAAAAGCAGCCATTCCCCTTTCCCCTT; CCCCATGTTTTTCACTTAAGTATAAGAGATGGTGAGCTCTGTGTTGGTAGGGATGTTCAT; CTTACGTGCCAGACTGACCTANCACAGGTGTTTGTTTGTTTGTTTGTATTGGAGTTTTGC; TCTCGTTGCCTAAGCCGGAGTGCAGTGGTGCCATCTCAGCTCACTGCAACCTCCACCTCC; CAGGTTCAAGCGATTCTCATGCCTTGGCCTCTCGAGTAGCTGGGATTACAGGTGCACACC; ACCACGCTCAGCAAATTTTTGTATCTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGG; CTGGTCTTGAACTCCTGACCTCAGATGACCCACCCACCTTGGCCTCCCAAAGTGCTGGGA; TTACAAGTGTGAGCCACCATGCCCGGGCCGCATAGGTTCTTAATAAATATCTATTGAATG; AATCAACCATCTTTAAAGGTTACAAGTACCCACTGCCCCCCGTGCCCCACCAGTCTACTG; CCTGACTTCAGTCTCCTACACTAAATTCCAAGTGACCTCAAGATGCGTCTTTTATTGGGA; TGGGTTCTGGCAAGNCCTAAAACTAAACTTTTTTTGCAGCCAATGCTAATGAACCTGCCT; GGAGGCTTGGCTTGGCCAGCCACTTTGGGTCCTTTTGATTCGCCAAATGCAGCTCCCGGT; TTTATTTGGAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_021181
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021181.3, NP_067004.3
• RefSeq Size: 2672 bp
• RefSeq ORF: 1008 bp
• Locus ID: 57823
• UniProt ID: Q9NQ25
• Cytogenetics: 1q23.3
• Protein Families: Druggable Genome, Transmembrane
• Summary: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as CS1-L). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.