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MASA (ENOPH1) (NM_021204) Human Untagged Clone

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SKU
SC112927
ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MASA
Synonyms E1; MASA; MST145; mtnC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112927 sequence for NM_021204 edited (data generated by NextGen Sequencing)
ATGGTCGTGCTTTCGGTCCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACC
ACAACCCCGATTGCTTTCGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAA
GAGTATCTGCAGACACATTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGG
AAACAGGCTGAAGAGGACGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGG
AATGGAGTGGATGATCTGCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAG
ATGTCCCTGGATCGAAAGACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCG
GCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCAGG
AAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAGGCACAG
AAACTGTTATTCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTT
GATACCAAGATTGGACACAAAGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATT
GGGTGCTCAACCAACAACATTTTGTTTCTGACAGATGTTACTCGAGAGGCCAGTGCTGCT
GAGGAAGCAGATGTGCACGTAGCTGTGGTGGTGAGACCAGGCAACGCAGGATTAACAGAT
GATGAGAAGACTTACTACAGCCTCATCACATCCTTCAGTGAACTATACCTGCCTTCCTCA
ACCTAG

Clone variation with respect to NM_021204.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_021204 unedited
AGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGGTGTGCAG
AAGTGTCCTCTCCCCACGCGCGGCGGGCTGCACTTGGTCGCTGGCTCCGAGATCGCGCGG
GGCCGCCGGAAGCCCAAGACGGTACCGGGGGCCGCAGCCGCAGCCGGCGCCGCCCTCCGC
CCTCCCCAACAGCAGGCCGAGTCCCGTAGCATCCGGTAGGGAAATGGTCGTGCTTTCGGT
CCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACCACAACCCCGATTGCTTT
CGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAAGAGTATCTGCAGACACA
TTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGGAAACAGGCTGAAGAGGA
CGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGGAATGGAGTGGATGATCT
GCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAA
GACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCGGCATTCACAGCTGGGCG
CATGANAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCANGAAGTGGAGAGAGGCCGG
AATGAANGTGTACATCTATTCCTCAGGNAGTGNTGANNGCACAGAAACTGNNTATTCGGC
ATTTCTACGAGGGAGATATTTCTTGAGCTGTTGATGGTCACTTTGATACCCAGATTGGAC
ACANAGTAGAGAGTGAAAGTTACCGAAAGATGCAGACAGCATTGGGTGCTCAACCACCAC
ATTTTGTTTCTGACAGATGTTACCTCCAAAGGCCAGTGCTGCTGAAGAAGCAAATGTGCA
CGTAGCTGTGGTGGTGAAACCAGGCACCGCAGATTACAGATGATGAGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_021204 unedited
NNNATTTTTTAGCTTGNACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTT
TGAAGAAAAAAGCTTTTATTAATCACAACATGAAATTAACATGACATCGTATTAGAATTA
AACCAGGATAAATACTGCTGTTATAAAATTTACATTTCTCACATCCATTCCAGAGGAAAA
TCTTACATATTAGGGCTCATCTTAATGTTATGGACTGATTTCAGTAAAACTTTTTAAATA
GTAAATAGCAATTAACGTATTCTCAAACTGTGCTAAGTAGTTAGAAAGGCAACTATAAAA
TCTATAATGATAACACGTGGCAGGATTAGAACTGTTCTGTTAAACATTTAAGAACCACAT
CTTTGTTTTAGATGTTACCTCAGTGCACCTTTTTCACTGGTAAGGCACCTGCTTGCTGTG
GGTGCCCCATTGTTACCCAGGTTTCTTTTTAGTAAAAACCCTTGGTTGAACAAACCTAAG
GGAGTTTTCTTTCCCATTTTGGCCTTTGAACCGAAAGGAAACCCTGGGCCTTGGTTTTTT
AAGGGGGCCATTTGAAACCCCCTGTTTAATTTTTTAATCCACGGGGCCTAAAATTTCCTT
TCTTTTAAAAAATTCCCCTTTAAAAATTTTATTTTTAAAAAAACGGGCCTTAAAAAAACC
TCAAACCCCCTTTCCCCTTTTCCCCCCCTTTTTAACCCTCCCCCCCCTAAAGGGGTTTTA
AAATTTTTTTCACCCACCCCCTTTCCCCCCCTTTGGGGGGAGAAAGATTTTAAAAAAATT
TCCCCCAAAAAAACCTTTTAAAAAAATTGTGTGGCCCCCAAAAAAAATTTTTTTTTTTTT
ATCCCCGGGG
Restriction Sites NotI-NotI |
ACCN NM_021204
Insert Size 1990 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021204.1, NP_067027.1
RefSeq Size 1992 bp
RefSeq ORF 786 bp
Locus ID 58478
UniProt ID Q9UHY7
Cytogenetics 4q21.22
Domains Hydrolase
Protein Pathways Cysteine and methionine metabolism
Summary Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_021204" in other vectors (5)
SKU Description Size Price
RC209510 ENOPH1 (Myc-DDK-tagged)-Human enolase-phosphatase 1 (ENOPH1) 10 ug
$300.00
RC209510L3 Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), Myc-DDK-tagged 10 ug
$600.00
RC209510L4 Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), mGFP tagged 10 ug
$600.00
RG209510 ENOPH1 (tGFP-tagged) - Human enolase-phosphatase 1 (ENOPH1) 10 ug
$489.00 $500.00
SC321962 ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1) 10 ug
$300.00

file-alt Citations

Role for human arylamine N-acetyltransferase 1 in the methionine salvage pathway
Witham, Minchin, Butcher
Biochem. Pharmacol. (2016)
AI Summary: OriGene’s siRNA was used in this study to knock down target genes, enabling the researchers to investigate the role of NAT1 and MRI-1 in the methionine salvage pathway and cancer cell growth.
Snippet: When cell density reached 50% confluence, 20 nM siRNA (Origene, Rockville,Maryland, USA) was transfected into the cells using Lipofectamine RNAiMax (Invitrogen,Melbourne, Victoria, Australia) in accordance with the manufacturer's instructions. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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