Dynamitin (DCTN2) (NM_006400) Human Untagged Clone

SKU SC112693

DCTN2 (untagged)-Human dynactin 2 (p50) (DCTN2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Dynamitin
• Synonyms: DCTN50; DYNAMITIN; HEL-S-77; RBP50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112693 sequence for NM_006400 edited (data generated by NextGen Sequencing); ATGGCGGACCCTAAATACGCCGACCTTCCCGGCATTGCCAGGAATGAGCCAGATGTTTAT; GAAACTAGCGACCTACCTGAGGATGATCAAGCGGAGTTCGATGCGTTTGCACAAGAGCTG; GAGGAGCTGACAAGCACAAGTGTGGAACACATCATTGTCAATCCTAATGCTGCCTATGAC; AAGTTCAAGGACAAGAGAGTGGGGACAAAGGGACTTGATTTCTCAGATCGTATTGGAAAA; ACCAAGAGGACAGGATATGAATCTGGAGAATATGAGATGCTTGGAGAGGGTCTGGGAGTG; AAGGAGACACCCCAGCAAAAGTACCAGCGCCTACTGCATGAGGTCCAAGAGCTGACAACT; GAAGTTGAAAAAATCAAGACGACAGTGAAGGAGTCAGCCACAGAGGAGAAGCTGACCCCT; GTGTTGCTGGCTAAACAGCTGGCAGCCCTGAAGCAGCAGCTGGTTGCTTCCCACCTGGAG; AAGCTGCTGGGACCAGATGCTGCAATCAACCTTACCGACCCCGATGGCGCCCTGGCTAAG; CGCCTACTACTGCAGCTGGAAGCAACAAAGAACAGCAAAGGGGGATCAGGGGGAAAAACC; ACTGGGACCCCCCCAGATAGCAGCCTTGTCACTTATGAACTACATTCTCGGCCTGAGCAG; GACAAGTTCTCTCAAGCTGCCAAAGTCGCAGAACTTGAAAAGCGCCTGACAGAGCTGGAG; ACAGCTGTACGTTGTGATCAGGATGCTCAGAATCCCCTTTCTGCAGGTCTACAGGGAGCC; TGTCTCATGGAGACTGTAGAGCTGTTGCAAGCAAAGGTGAGCGCCCTAGACCTTGCAGTT; TTGGATCAAGTGGAGGCTCGGCTACAGAGTGTCCTGGGAAAGGTGAACGAGATTGCCAAG; CATAAAGCCTCTGTAGAAGATGCAGATACACAAAGCAAGGTGCACCAGCTATATGAAACT; ATACAGCGCTGGAGCCCCATTGCCTCCACCCTCCCTGAGCTGGTGCAGAGACTTGTCACC; ATCAAGCAGCTGCACGAGCAAGCCATGCAGTTTGGTCAGCTCCTGACACACTTGGATACC; ACCCAGCAGATGATTGCTAATTCCTTGAAGGACAATACCACCCTCTTGACCCAGGTGCAG; ACAACCATGCGTGAAAACCTGGCCACAGTTGAGGGGAACTTTGCCAGCATTGATGAACGG; ATGAAGAAGCTGGGAAAGTGA; ; Clone variation with respect to NM_006400.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006400 unedited; TATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTCCCTTTGCCGCCGCC; TTAGCCCGGGACCCGAACCCAGCCTCTCCCCTACCCGAACACCGGCCCCGGCTCCACCGA; GGCCCGGGTCCCCCAGCCCGTCTCGCCGCCGCCATGGCGGACCCTAAATACGCCGACCTT; CCCGGCATTGCCAGGAATGAGCCAGATGTTTATGAAACTAGCGACCTACCTGAGGATGAT; CAAGCGGAGTTCGATGCGTTTGCACAAGAGCTGGAGGAGCTGACAAGCACAAGTGTGGAA; CACATCATTGTCAATCCTAATGCTGCCTATGACAAGTTCAAGGACAAGAGAGTGGGGACA; AAGGGACTTGATTTCTCAGATCGTATTGGAAAAACCAAGAGGACAGGATATGAATCTGGA; GAATATGAGATGCTTGGAGAGGGTCTGGGAGTGAAGGAGACACCCCAGCAAAAGTACCAG; CGCCTACTGCATGAGGTCCAAGAGCTGACAACTGAAGTTGAAAAAATCAAGACGACAGTG; AAGGAGTCAGCCACAGAGGAGAAGCTGACCCCCTGTGTTGCTGGCTAAACAGCTGGCAGC; CCTGAAGCAGCAGCTGGGTTGCTTCCACCTGGAGAAGCTGCTGGGACCAGATGCTGCAAT; CAACCTTACCGACCCCGATGGCGCCCTGGCTAAGCGCCTACTACTGCAGCTGGGAAGCAC; AAGAACAGCAAAGGGGGATCAGGNGGAAAAACACTGGGACCCCCCAGATAGCAGCCTTGT; CACTTATGAACTACATTCCTCGNCTGAGNCAGGGACAGTCTCTCAAGCTGCCAAAGTCGC; AAAACTGAAAAGCGCCTGACAGAGCTGAAGACAGCTGTACGTTGGGATCAGGATGCTCAN; AATCCCCTTCCTGCACGTCACAGGGGACCCTGTCTATGGAGACTGTTNAGCTGTGCAGCA; AAGTGGACGCCCTAAACTTGCAGTTTTGGACAGTGGNAGCTCGCTTACAATTGCCTGGGA; AGGGGANANATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006400 unedited; CGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAGGATACAACAGCATTTA; ATGGGTCAGAAACAGTTGTACAGTATTACAGTCAGCCACAGAAGCTGTGTTGGGGGACAA; GACCCAATCCTTCCCCACACCAGGCAAAGCAGTATTGGACATGAGTTGGCATGTGGCTGG; GCCCACGTCCTTATCCCCCAGGCCTGAGGGGAGACCACCTTCTGATGATAACCAACCCCT; AGCTACCACTCTGTATTCATCAGGGGAGGGGTATAAACCCCACATGCAAGAAGAACCCTT; GCCCCCAGTGTCAAATGGGATGGGGATGCTAGAGTTATAGTAAAGGGGAAACCCTATGTA; AGCTGTTAACAGAGTTCACAGGGGTAGGGATAACCCCTGTTCTCCAGCTCCCAAATGTGC; TCACTTTCCCAGCTTCTTCATCCGTTCATCAATGCTGGCAAAGTTCCCCTCAACTGTGGC; CAGGTTTTCACGCATGGTTGTCTGCACCTGGGTCAAGAGGGCGGTATTGTCCTTCAAGGA; ATTAGCAATCATCTGCTGGGTGGTATCCAAGTGTGTCAGGAGCTGACCAAACTGCATGGC; TCGCTCGTGCAGCTGCTTGATGGAGACAAGTCTCTGCACCAGCTCAGGGAGGGCGGAGGC; AATGGGGCCTCAGCGCCGAATAGTCTCATATAGCCGGCGCCCCCTGCTTTGCGTATTTGC; CTCTTCTACAGAGGCTTTATGCCTTGGCAATCTGGTCCACCTTCCCCAGGACACTCTGTA; CCCCAGCCTCCACTTGACCAAAACTGCAAGGCTAGGTCGCTCACCTTTGCTTGCAACAAC; CTTACAGTCCCCTGAGACAGGCTNCCTGTACACCTGCGAAAGGGAGATTTGGACCTTCTG; AAACAACCGACAAGTGTCTCACACTCTGTAGGGCCCTTTAACTCCCGCACTTGGGACCTG; GAGAAACTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006400
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006400.3, NP_006391.1
• RefSeq Size: 1757 bp
• RefSeq ORF: 1221 bp
• Locus ID: 10540
• UniProt ID: Q13561
• Cytogenetics: 12q13.3
• Domains: Dynamitin
• Protein Pathways: Huntington's disease
• Summary: This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]; Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.