RASA3 (NM_007368) Human Untagged Clone

SKU SC112652

RASA3 (untagged)-Human RAS p21 protein activator 3 (RASA3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RASA3
• Synonyms: GAP1IP4BP; GAPIII
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112652 sequence for NM_007368 edited (data generated by NextGen Sequencing); ATGGCGGTGGAGGACGAGGGGCTCCGGGTCTTCCAGAGCGTGAAGATCAAGATCGGTGAA; GCCAAAAACCTTCCCTCTTACCCGGGGCCGAGCAAGATGAGGGATTGCTACTGCACGGTG; AACCTGGACCAGGAGGAGGTTTTCAGGACCAAAATTGTGGAAAAGTCACTCTGCCCGTTT; TACGGAGAAGACTTTTACTGTGAAATTCCTCGGAGCTTTCGTCACCTGTCCTTCTACATT; TTCGATAGAGACGTTTTCCGGAGGGATTCCATCATAGGGAAGGTGGCCATCCAGAAGGAG; GACTTGCAGAAGTACCACAACAGGGACACCTGGTTCCAGCTGCAGCACGTGGACGCTGAC; TCGGAAGTGCAGGGCAAAGTGCACCTGGAGCTGCGGCTGAGCGAGGTCATCACAGACACT; GGGGTCGTCTGCCACAAGCTCGCCACACGCATCGTCGAGTGCCAGGGCCTCCCCATCGTG; AATGGGCAATGTGACCCCTACGCCACCGTGACGCTGGCAGGACCCTTCAGATCAGAAGCA; AAGAAGACGAAAGTGAAGAGGAAGACCAACAATCCCCAGTTCGATGAAGTGTTTTATTTT; GAGGTGACCCGGCCCTGTAGCTACAGCAAGAAGTCCCACTTTGACTTTGAGGAGGAAGAC; GTGGACAAGCTCGAAATCAGAGTTGACCTCTGGAATGCCAGTAACCTGAAGTTTGGAGAT; GAATTCCTGGGAGAACTAAGGATCCCGTTGAAAGTCCTGCGGCAGTCCAGCTCCTACGAG; GCGTGGTACTTCCTCCAGCCCCGGGACAATGGTAGCAAGAGCCTAAAGCCAGACGACCTG; GGCTCCCTGCGGCTGAACGTGGTATACACGGAAGACCACGTGTTTTCTTCTGACTATTAC; AGCCCTCTGCGGGACCTGCTGTTGAAGTCTGCGGATGTGGAGCCCGTGTCAGCGTCTGCG; GCCCACATCCTGGGCGAGGTTTGCCGGGAGAAGCAGGAGGCGGCCGTCCCGCTGGTGCGG; CTCTTCCTACACTATGGCAGGGTGGTGCCATTCATCAGTGCCATCGCCAGCGCGGAGGTG; AAGCGGACCCAGGACCCCAACACCATCTTCCGAGGAAACTCACTGGCGTCCAAGTGCATC; GACGAGACCATGAAGCTGGCGGGGATGCATTACCTGCATGTCACCCTGAAGCCCGCCATC; GAGGAGATATGCCAGAGCCACAAACCCTGTGAAATCGACCCTGTGAAGTTGAAAGACGGA; GAAAACCTTGAAAACAACATGGAGAACCTACGGCAGTATGTGGACCGCGTCTTCCACGCC; ATCACCGAGTCTGGGGTGAGCTGCCCGACCGTCATGTGTGACATCTTCTTCTCCCTCCGG; GAGGCGGCGGCCAAGCGCTTCCAGGATGACCCGGACGTCAGGTACACTGCAGTGAGCAGC; TTCATCTTCCTGAGGTTCTTTGCGCCCGCCATTCTCTCCCCCAACCTCTTCCAGCTCACG; CCGCACCACACGGACCCCCAGACGTCCAGGACGCTGACATTGATCTCCAAGACCGTTCAG; ACCCTCGGCAGCCTGTCCAAGTCCAAATCTGCGAGTTTTAAGGAGTCCTACATGGCTACA; TTTTATGAATTCTTCAATGAGCAGAAATATGCTGATGCGGTGAAGAACTTCTTGGATCTG; ATTTCGTCCTCGGGGAGAAGAGACCCCAAGAGTGTTGAGCAGCCCATCGTGCTTAAAGAA; GGGTTCATGATCAAGAGGGCCCAAGGACGGAAGCGCTTTGGGATGAAGAATTTTAAGAAG; AGATGGTTTCGCTTGACCAACCATGAATTTACCTACCACAAAAGCAAAGGGGACCAGCCT; CTCTACAGCATTCCCATCGAGAACATCCTGGCAGTGGAGAAGCTGGAGGAGGAGTCTTTC; AAAATGAAAAACATGTTCCAGGTCATCCAGCCAGAGCGTGCGCTGTACATCCAGGCCAAC; AACTGCGTGGAGGCCAAGGACTGGATCGACATTCTCACCAAAGTGAGCCAGTGCAACCAG; AAGCGCCTCACCGTCTACCACCCGTCCGCCTACCTGAGCGGCCACTGGCTGTGCTGTAGG; GCGCCATCCGACTCGGCTCCGGGCTGCTCGCCCTGCACTGGCGGCCTCCCAGCCAACATC; CAGCTGGACATTGATGGGGACCGTGAGACGGAGCGTATCTACTCCCTCTTCAACTTGTAC; ATGAGCAAGCTGGAGAAGATGCAGGAGGCCTGTGGGAGCAAATCTGTGTATGACGGCCCG; GAGCAGGAGGAGTATTCGACGTTCGTCATTGACGACCCCCAGGAGACCTACAAGACGCTA; AAGCAAGTCATCGCTGGGGTTGGGGCTTTGGAGCAGGAGCACGCCCAGTATAAGAGGGAC; AAGTTCAAGAAGACGAAATATGGAAGCCAGGAGCACCCCATCGGAGACAAGAGCTTCCAG; AACTACATCCGGCAGCAGTCCGAGACCTCCACTCATTCCATTTAA; ; Clone variation with respect to NM_007368.2; 1326 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007368 unedited; ATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGCTGCCCGGGCTCGGCGCGCGCTTGGGG; CGAGGCTCGGCGGGCGCGGACGCGCAGCATGGCGGTGGAGGACGAGGGGCTCCGGGTCTT; CCAGAGCGTGAAGATCAAGATCGGTGAAGCCAAAAACCTTCCCTCTTACCCGGGGCCGAG; CAAGATGAGGGATTGCTACTGCACGGTGAACCTGGACCAGGAGGAGGTTTTCAGGACCAA; AATTGTGGAAAAGTCACTCTGCCCGTTTTACGGAGAAGACTTTTACTGTGAAATTCCTCG; GAGCTTTCGTCACCTGTCCTTCTACATTTTCGATAGAGACGTTTTCCGGAGGGATTCCAT; CATAGGGAAGGTGGCCATCCAGAAGGAGGACTTGCAGAAGTACCACAACAGGGACACCTG; GTTCCAGCTGCAGCACGTGGACGCTGACTCGGAAGTGCAGGGCAAAGTGCACCTGGAGCT; GCGGCTGAGCGAGGTCATCACAGNACACTGGGGTCGTCTGCCACAAGCTCGCCACACGCA; TCGTCGAGTGCCAGGGCCTCCCCATCGTGAATGGGCAATGTGACCCCTACGCCACCGTGA; CGCTGGCAGGACCCTTCAGATCAGAAGCANAGAAGACGAAAGTGAAGAGGAAGAACCAAC; ATCCCCAGTTCGATGAAGTGTTTTATTTTGAGGTGACCCCGGCCCTGTAGCTACAGCAAG; AAGTCCCACTTTGACTTTGAGGAGGAAGACGTGGNACAGCTCGAAATCAGAGTTGACCTC; TGGAATGCCAGTACCCTGAAGTTTGGAGATGAATTCTGGGAGAACTAAGATNCCGTTGAA; GTCCTGCGGCATCCAGTTCTACCAGGCGTGTACTTTCTCCAGCCCGGGACATGGTACAGA; GCCTAACCAGACACTGNGCTCCTGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007368 unedited; TAAACATCTGTTTTAATTGGGGCCAGTAAAATACATTGGCAAATCATTAGTATTTTATAA; ACAGAAACATTAAGTTATAAATCCATGTTAAAAATTGCAGCTCAGTGCAGCCCCAACAGC; TGTGTGTCCAAAAGTCTATTTTTACAACATTTTCCTCCAGTATTTTTGTGACAAGAAAGT; TCCTATCTGCCATCTAGATCAAGAGCCTGAACTACAGTATTTTCAAACAAGAACCGGGAG; AAGTCGGTACAAGGATACGTCGTGCTGTGTATTTAAACGGCGCTTCCCACACACCTGCCG; TCTGCATCCCGGGAGGACGCCCTTTGGGAAAGGCCTCCTCGGCAGTGTGAGGTCTGGAGG; GGTCTGCCTGGACCCCATTTTTAGGATGCTCCCCTCCCCAATAAAACAGCAGCCCCCGCC; CCTGCCCTGCACAGCATCTGGGGGTTCATAACACAGGTGCAATACTGACCATGGGCCCTG; GAGGGACGTCTGCACCCCGAAGCCCCTGTACCTCAAACTCAGAGTTTCTTCCCTTCTTTG; ATTTTCTGGAGGACCTGCAGCTGGCCTTCCTGAGACAGGCTCCATTCCTGTTCCATTTGC; CTTCCCGGCAGCCTTCCCTCTAGTGGGTATAGGTTCTGACGTTCTGAGTTACTTTGTATC; AAAGAGCTAATTAAAAATGGTTCTTCACAAACATAAAGAAAAACAGCTTGAAACGGTACA; TCTTCCTATTTTTAAATCTTCAACCTTCCACGGGGGGGTGGCCAATGTTAATTCTGATTT; TAAACCCCTTTAACAGCAAACGATGTGGGCTTCAACTGTGGGCTTCCGAACACCCTGGAG; ACCTGGAGCCACCCTTTTCCTCCAGGGACTCTGGGCAAACCTGTTTCCCCAGCCCAAGCA; CTCTCAACTGGACTTTGGGTATCTGCCTCCAGCTTAACGGCCCTATCGGAATAACAACCC; GAGGGGACGGGGCCGCTTCTGTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_007368
• Insert Size: 4110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007368.2, NP_031394.2
• RefSeq Size: 4220 bp
• RefSeq ORF: 2505 bp
• Locus ID: 22821
• UniProt ID: Q14644
• Cytogenetics: 13q34
• Domains: BTK, C2, PH, RasGAP
• Summary: This gene encodes a protein that binds inositol 1,3,4,5-tetrakisphosphate and stimulates the GTPase activity of Ras p21. This protein functions as a negative regulator of the Ras signalling pathway. It is localized to the cell membrane via a pleckstrin homology (PH) domain in the C-terminal region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (1) encodes the longest isoform (1).