NFYC (NM_014223) Human Untagged Clone

SKU SC112622

NFYC (untagged)-Human nuclear transcription factor Y, gamma (NFYC), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NFYC
• Synonyms: CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014223, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCCACAGAAGGAGGATTTGGTGGTACTAGCAGCAGTGATGCCCAGCAAAGCCTACAGTCGTTCTGGC; CTCGGGTCATGGAAGAAATCCGGAATTTAACAGTGAAAGACTTCCGAGTGCAGGAACTCCCACTGGCTCG; TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCGCCTGTACTCTTTGCC; AAGGCAGCCCAGATTTTTATCACAGAGTTGACTCTTCGAGCCTGGATTCACACAGAAGATAACAAGCGCC; GGACTCTACAGAGAAATGATATCGCCATGGCAATTACAAAATTTGATCAGTTTGATTTTCTCATCGATAT; TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGTCAGGAGGAGGTGCGCCAGTCTGTAACTCCTGCCGAG; CCAGTCCAGTACTATTTCACGCTGGCTCAGCAACCCACCGCTGTCCAAGTCCAGGGCCAGCAGCAAGGCC; AGCAGACCACCAGCTCCACGACCACCATCCAGCCTGGGCAGATCATCATCGCACAGCCTCAGCAGGGCCA; GACCACACCTGTGACAATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTCCAGGCTCAGCCACAGGGT; CAAGCCCAACAGGCCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATCACTAACACAG; GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCCGGCCAGCTGCAGTATATCCGCTTAGCCCAGCCTGT; ATCAGGCACTCAAGTTGTGCAGGGACAGATCCAGACACTTGCCACCAATGCTCAACAGATTACACAGACA; GAGGTCCAGCAAGGACAGCAGCAGTTCAGCCAGTTCACAGATGGACAGCAGCTCTACCAGATCCAGCAAG; TCACCATGCCTGCGGGCCAGGACCTCGCCCAGCCCATGTTCATCCAGTCAGCCAACCAGCCCTCCGACGG; GCAGGCCCCCCAGGTGACCGGCGACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014223 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTGCTCTTCCTGGA; CTCCTGAGCAGAGTTGTCGAGATGTCCACAGAAGGAGGATTTGGTGGTACTAGCAGCAGT; GATGCCCAGCAAAGCCTACAGTCGTTCTGGCCTCGGGTCATGGAAGAAATCCGGAATTTA; ACAGTGAAAGACTTCCGAGTGCAGGAACTCCCACTGGCTCGTATTAAGAAGATTATGAAA; CTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCGCCTGTACTCTTTGCCAAGGCAGCC; CAGATTTTTATCACAGAGTTGACTCTTCGAGCCTGGATTCACACAGAAGATAACAAGCGC; CGGACTCTACAGAGAAATGATATCGCCATGGCAATTACAAAATTTGATCAGTTTGATTTT; CTCATCGATATTGTTCCAAGAGATGAACTGAAACCTCCAAAGCGTCAGGAGGAGGTGCGC; CAGTCTGTAATTCCTGCCGAGCCAGTCCAGTACTATTTCACGCTGGCTCAGCAACCCACC; GCTGTCCAAGTCCAGGGCCAGCAGCAAGGCCAGCAGACCACCAGCTCCACGACCACCATC; CAGCCTGGGCAGATCATCATCGCACAGCCTCAGCAGGGCCAGACCACACCTGTGANCATG; CANGTTGGAGAAGGTCAGCAGGTGCAGATTGTCCAGGCTCAGCCACAGGGTCAAGCCCAA; CAGGCCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATCACTAACACA; GGAGAGACCAGCAGATCCCGGTGCAGCTGAATGCCGGCCAGCTGCAGTATATCCGCTTAG; CCCACCTGTATCAGCACTCCAGTGTGCAGGGACGATCCGACACTTGCACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014223 unedited; CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTAAAATGAGATTATTGCATT; TCATGCAGCTTGAAGTCCATGCAAAGGAGACTAGCACAGTTTTTAATGCATTTAAAAAAT; AAAAGGGAGGTGGGCAGCAAACACACAAAGTCCTAGTTTCCTGGGTCCCTGGGAGAAAAG; AGTGTGGCAATGAATCCACCCACTCTCCACAGGGAATAAATCTGTCTCTTAAATGCAAAG; AATGTTTCCATGGCCTCTGGATGCAAATACACAGAGCTCTGGGGTCAAAGCAAGGGATGG; GGAGAGGACCACGAGTGAAAAAGCAGCTACACACATTCACCTAATTCCATCTGAGGGCAG; AACAACGTGGCAAGTCTTGGGGGTAGCAGCTGTTCCCAGAGTCAAGAAATACCGTACACC; CCTGGGGAGATTTGCTCCTAAAAAGAAGTGGCAGTAACAAACAAAAAAAAAGAAAAACAA; AAGGAGAAATATTTCTTCAACCACACTCTTCAAGAAAATGCCATAAATATGTTATTTAAT; ATTTTAATTTCATAGCATTGGACACACACTCAACATATTGAAATATTGATTCCTTAGAGA; AAAAAATGGAAAAAAAGGAAACAAAAAATATTGCATCTTTCTGCTGGAGAATCTCGGCCC; CCAGGCATGGGGTGTAGTGCACCAGTGTCCTANCCTGCAGGAGGCGCTGAGGTCGGCCGG; GTCCTCTGGGGAGAAGGCTGTGCCCATTGCCTNGGGCTGTATGGAAAAATGTGTTGGGTG; TCCTTGGCCTTGCAGCTCAGCCCTCATCGCCGGTCACTGGGGGGCCTGCCCGTCGAGGCT; TGGTGCTGACTGATGACATGGCTGGGGCAGGTCTGNCCCCAGCATGGGACTTGCTGTATC; TGGTAAACTCTGTCATTGGAACTGGTGAATGCTGTGGCCTGCTGACCTTGCTGGGAATTG; TGACATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014223
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014223.2, NP_055038.2
• RefSeq Size: 1965 bp
• RefSeq ORF: 1008 bp
• Locus ID: 4802
• UniProt ID: Q13952
• Cytogenetics: 1p34.2
• Domains: CBFD_NFYB_HMF
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation
• Summary: This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) has the same N- and C- termini but lacks an internal segment, compared to isoform 1.