PTCD1 (NM_015545) Human Untagged Clone

SKU SC112604

PTCD1 (untagged)-Human pentatricopeptide repeat domain 1 (PTCD1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: PTCD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112604 sequence for NM_015545 edited (data generated by NextGen Sequencing); ATGGACTTCGTGAGACTCGCTCGACTGTTCGCCAGGGCCCGCCCCATGGGACTGTTCATC; CTGCAACACCTGGACCCCTGTAGAGCCAGGTGGGCAGGAGGCAGGGAGGGGCTGATGCGG; CCAATGTGGGCGCCCTTCAGCAGCTCCTCCTCTCAGCTGCCCCTCGGCCAGGAGCGTCAG; GAAAACACGGGCAGCCTGGGCTCTGACCCGAGCCACTCCAACTCCACGGCCACGCAGGAA; GAAGACGAGGAGGAGGAGGAGAGTTTTGGGACCCTCTCTGACAAATACTCCTCCCGGAGA; CTATTCCGCAAATCCGCAGCCCAGTTCCATAACCTGCGGTTTGGGGAACGGAGAGATGAG; CAAATGGAACCGGAGCCCAAATTATGGCGAGGCCGGAGAAACACCCCATACTGGTACTTC; TTGCAGTGCAAACACCTGATCAAGGAAGGGAAGCTGGTTGAAGCCCTGGACCTGTTTGAG; AGGCAGATGCTGAAGGAGGAGCGATTGCAGCCCATGGAGAGCAACTACACGGTGCTGATT; GGGGGCTGCGGGCGGGTTGGCTACCTGAAGAAGGCCTTCAACCTCTACAACCAGATGAAA; AAGCGGGACCTGGAGCCCTCGGACGCCACCTACACGGCCCTGTTCAACGTCTGTGCCGAG; TCCCCCTGGAAGGACTCAGCTCTACAGAGCGCCCTGAAGCTCCGGCAGCAGCTGCAGGCC; AAAAACTTCGAGCTCAACTTGAAAACATACCACGCGCTGCTGAAGATGGCTGCCAAGTGC; GCAGACCTTAGGATGTGCCTCGATGTGTTCAAGGAAATCATCCACAAAGGGCACGTGGTC; ACAGAGGAGACCTTCAGTTTCCTGCTCATGGGCTGCATCCAAGACAAGAAGACAGGCTTC; CGGTACGCCCTCCAGGTGTGGCGGCTGATGCTGAGTCTAGGGCTACAGCCGAGCCGGGAC; AGCTACAACCTGCTGTTGGTGGCAGCTCGGGACTGTGGCCTAGGGGACCCCCAGGTGGCC; TCAGAGCTGCTTCTGAAGCCCAGGGAGGAGGCGACTGTGCTTCAGCCCCCAGTGAGCAGG; CAGCGGCCAAGGAGGACAGCCCAGGCCAAGGCAGGCAACCTCATGTCAGCCATGCTGCAT; GTGGAGGCCCTGGAGAGGCAGCTGTTTCTGGAACCTTCTCAGGCACTTGGGCCTCCAGAG; CCTCCGGAAGCCAGAGTGCCCGGCAAGGCCCAACCAGAGGTGGATACTAAGGCAGAGCCC; AGCCACACAGCAGCCCTCACCGCAGTGGCCCTGAAGCCACCTCCCGTGGAGCTGGAAGTC; AACCTCCTGACCCCCGGGGCCGTTCCCCCTACAGTGGTCTCCTTTGGAACGGTGACCACC; CCAGCTGACCGGCTGGCCTTGATAGGGGGCCTGGAGGGCTTCCTGAGCAAGATGGCAGAG; CACAGGCAGCAGCCCGACATCAGGACCCTCACGCTACTGGCCGAGGTGGTGGAGTCCGGG; AGTCCTGCAGAGTCCTTGCTGCTGGCCCTCCTGGATGAGCACCAGGTAGAGGCCGACCTG; ACATTCTTTAACACGCTGGTGAGAAAGAAGAGCAAGCTGGGAGACCTGGAGGGGGCCAAG; GCGCTGTTGCCGGTCCTGGCAAAGAGGGGCCTCGTCCCCAACCTGCAGACATTCTGCAAC; CTGGCCATCGGGTGCCACAGGCCGAAGGACGGTCTACAGCTTCTCACAGACATGAAGAAG; TCCCAGGTGACCCCCAACACTCACATCTACAGTGCCCTCATCAACGCGGCCATCAGGAAG; CTGAACTACACCTATCTCATCAGCATCTTGAAGGACATGAAGCAGAACAGGGTCCCGGTG; AACGAAGTGGTCATCCGCCAGCTGGAGTTTGCAGCCCAGTACCCTCCCACCTTTGACCGG; TACCAAGGGAAGAACACCTACCTGGAGAAGATTGACGGCTTCCGAGCCTATTACAAGCAG; TGGCTGACAGTGATGCCCGCAGAGGAAACCCCGCACCCCTGGCAGAAGTTCCGGACCAAG; CCCCAGGGGGACCAGGACACCGGCAAGGAGGCTGATGACGGATGTGCCCTTGGGGGCAGG; TGA; ; Clone variation with respect to NM_015545.3; 408 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015545 unedited; TGTCGCATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGGCAGGGCTC; CCCGTCGGAGTGCACTGCGCCGGACACTTCAAGCCCTGGAGGGACAGGAAAGCCAGAAAT; GGACTTCGTGAGACTCGCTCGACTGTTCGCCAGGGCCCGCCCCATGGGACTGTTCATCCT; GCAACACCTGGACCCCTGTAGAGCCAGGTGGGCAGGAGGCAGGGAGGGGCTGATGCGGCC; AATGTGGGCGCCCTTCAGCAGCTCCTCCTCTCAGCTGCCCCTCGGCCAGGAGCGTCAGGA; AAACACGGGCAGCCTGGGCTCTGACCCGAGCCACTCCAACTCCACGGCCACGCAGGAAGA; AGACGAGGAGGAGGAGGAGAGTTTTGGGACCCTCTCTGACAAATACTCCTCCCGGAGACT; ATTCCGCAAATCCGCAGCCCAGTTCCATAACCTGCGGTTTGGGGAACGGAGAGATGAGCA; AATGGAACCGGAGCCCAAATTATGGCGAGGCCGGAGAAACACCCCATACTGGTACTTCTT; GCAGTGCAAACACCTGATCAAGGAAGGGAAGCTGGTTGAAGCCCTGGACCTGTTTGAGAG; GCAGATGCTGAAGGAGGAGCGATTGCAGCCCATGGAGAGCAACTACACGTGCTGATTGNG; GGCTGCGGGCGGGTTGGCTACCTGAAGAGGCCTTCAACCTCTACACCAGATGAAAAAGCG; GGACCTGGAGCCCTCGGACGCCACCTACACGGNCCTGTTCAACGTCTGTGCCGAGTCCCC; CTGGAGGACTCAGCTCTACAGAGCGCCCTGAAAGCTCCGCAGCAGCTGCAAGCCAAAAAC; TTCGAGCTCAACTTGAAAACATACCACGCGCTGCCTGAGATGGGCTGCCAGTGCGCAGAC; TTTAGATGTGCCTCGATGTGTTCAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015545 unedited; TCTTCTGTCCCGCGGCCCGCACTCCTAGCACCGCGTTCTTTTTTTTTTTTTTTCTTGATT; GGCAAACGTGCGTTGGATTTGCAGCAACATATCATAAATAGACATACAGATGTAACCTCG; GTGTCAACAGCAGCTGGTTCTACCTTCATGAGGGAGCCAAATTTTCCCTGCCCAGACCTG; CCACGGAAGGGTTCCTGAGGTGTGTCCCTATATGGCATGTTGTCAGCCCCCTCTTGTCCC; CTCTGCCTCCCCCCATTCCCCCCCATTTTCCCCCTCCCCCCCCCCTTTTTTCCCCTTTCC; CCTCTCCCTCCCTTTTCCCCCCTTCCCCCCTTTTTCCCCCCCCCCCCTCCCCCCTTTTCT; CTCTCCCCTTCCCCCCCCTTCCTTTTTTTTTTTTTTCCCCCCCTCTCCCCCCCCCCTCCC; TTCCCCCCCTTTTTTCCCTCCTTCCCCTCCCCTCCCCTTCTCGTCCCGCCCCCCCCCATT; ATTCTCTTTTTTCCCCCCCCCCCCCCCCCCCTTTTTTCCTTCCCCCCTCCCATTTTCCCC; CCCCTTCGTTCCCTTCCTCCCATTTTTTCCCCCCCCTCCCCCTTTTCCCCCCCTTCCCTT; CTCCCCTTCCCTCTTCCCATCCTCCTTTTCTCCTCTTCCCCTTCCCCCTTCCCCTCCCCT; CCCCCACCGACTTCTCCCCATTTCACTTCCGCCTTTTCCTTCCCTTCTCTCTCTTTCTTC; TCTTCCTCCTTCATCTTTTTCTCTCCCCCCTTTCCCCCGCTCCTCCTTCCCCTTTTCTAA; ATTCCCTCCTGTCTTTCCTCCACTTCCCTTTCCTCTCCCATTCCCCTTCTCTCCCCATCC; TTTTTTCCTCCCTTCCTTCGCGCTTCTTTTTCCTCTCGTCCCCCATCACCTCTCCCATCT; CAATCGCGCTCCGCTTACANATCTCACCTCTCCCCGCGCTTACTCCACCCCGCCTCCGTT; TTTCCCGTTCTCTTTTCTCCTCCCTCTCGCTCCCCCCTATCTTCTTTTCTCCATTCCCCC; CCTCATTCAGCTTTCCGTCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_015545
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015545.2, NP_056360.2
• RefSeq Size: 3079 bp
• RefSeq ORF: 2103 bp
• Locus ID: 26024
• UniProt ID: O75127
• Cytogenetics: 7q22.1
• Domains: PPR
• Summary: This gene encodes a mitochondrial protein that binds leucine tRNAs and other mitochondrial RNAs and plays a role in the regulation of translation. Increased expression of this gene results in decreased mitochondrial leucine tRNA levels. Naturally occurring read-through transcription exists between upstream ATP5J2 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2) and this gene. [provided by RefSeq, Aug 2015]