CDC25B (NM_021872) Human Untagged Clone
SKU
SC112556
CDC25B (untagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3
$503.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CDC25B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_021872 |
| Insert Size | 3120 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021872.2, NP_068658.1 |
| RefSeq Size | 3578 bp |
| RefSeq ORF | 1620 bp |
| Locus ID | 994 |
| UniProt ID | P30305 |
| Cytogenetics | 20p13 |
| Domains | RHOD |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation |
| Summary | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. provided by RefSeq, Jul 2008 Transcript Variant: This variant (3), also known as CDC25B2, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3) compared to isoform 1. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021872" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215766 | CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 | 10 ug |
$289.00
$540.00
|
|
| RC215766L3 | Lenti-ORF clone of CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 | 10 ug |
$840.00
|
|
| RC215766L4 | Lenti-ORF clone of CDC25B (mGFP-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 | 10 ug |
$840.00
|
|
| RG215766 | CDC25B (tGFP-tagged) - Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 | 10 ug |
$489.00
$740.00
|
|
Citations
F-box protein 7 mutations promote protein aggregation in mitochondria and inhibit mitophagy
Zhou, Xie, Sathiyamoorthy et al
Hum. Mol. Genet. (2015)
Zhou, Xie, Sathiyamoorthy et al
Hum. Mol. Genet. (2015)
Cited by 86
AI Summary: OriGene’s pCMV6-XL5-FBXO7 plasmid (Plasmid ID SC112556) was used as a source of wild-type FBXO7 for cloning and mutagenesis experiments in this study investigating the role of FBXO7 mutations in mitochondrial protein aggregation and mitophagy inhibition.
Snippet: The In-Fusion Advantage PCR Cloning Kit (Clontech) was used to clone isoform 1 and isoform 2 WT FBXO7 into pcDNA3-myc/His vector or pcDNA3-GFP vector (Addgene plasmid 13031) which was developed by Dr Doug Golenbock. The pCMV6-XL5-FBXO7 (Plasmid ID SC112556) was purchased from OriGene Technologies, Inc., USA. … FBXO7 mutants (R498X, R378G and T22M) and PARKIN R275W mutant were created based on the template of pcDNA3-FBXO7-GFP and pRK5-HA-PARKIN plasmids respectively by PCR-based technique using QuickChange site-directed mutagenesis kit (Stratagene, USA)
Showing 1-1 of 1 papers.
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