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5HT5A receptor (HTR5A) (NM_024012) Human Untagged Clone

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SKU
SC112321
HTR5A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol 5HT5A receptor
Synonyms 5-HT5A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112321 sequence for NM_024012 edited (data generated by NextGen Sequencing)
ATGGATTTACCAGTGAACCTAACCTCCTTTTCCCTCTCCACCCCCTCCCCTTTGGAGACC
AACCACAGCCTCGGCAAAGACGACCTGCGCCCCAGCTCGCCCCTGCTCTCGGTCTTCGGA
GTGCTTATTCTCACCTTGCTGGGCTTTCTGGTGGCGGCGACGTTCGCCTGGAACCTGCTG
GTGCTGGCGACCATCCTCCGTGTACGCACCTTCCACCGCGTGCCCCACAACCTGGTGGCA
TCCATGGCCGTCTCGGATGTCCTGGTGGCCGCGCTGGTCATGCCGCTGAGCCTGGTGCAC
GAGCTGTCCGGGCGCCGCTGGCAGCTAGGTCGGAGGCTGTGCCAGCTTTGGATCGCGTGC
GACGTGCTTTGCTGCACGGCCAGCATCTGGAACGTGACGGCCATAGCCCTGGACCGCTAC
TGGTCCATCACGCGCCACATGGAATACACGCTCCGCACCCGCAAGTGCGTCTCCAACGTC
ATGATCGCGCTCACCTGGGCACTCTCCGCTGTCATCTCTCTGGCCCCGCTGCTTTTTGGC
TGGGGAGAGACGTACTCTGAGGGCAGCGAGGAGTGCCAGGTAAGCCGCGAGCCTTCCTAC
GCCGTGTTCTCCACCGTAGGCGCCTTCTACCTGCCGCTCTGTGTGGTGCTCTTCGTGTAC
TGGAAGATCTACAAGGCTGCCAAGTTCCGCGTGGGCTCCAGGAAGACCAATAGCGTCTCA
CCCATATCCGAAGCTGTGGAGGTGAAGGACTCTGCCAAACAGCCCCAGATGGTGTTCACG
GTCCGCCACGCCACCGTCACCTTCCAGCCAGAAGGGGACACGTGGCGGGAGCAGAAGGAG
CAGCGGGCCGCCCTCATGGTGGGCATCCTCATTGGCGTGTTCGTGCTCTGCTGGATCCCC
TTCTTTCTCACCGAGCTCATCAGTCCCCTCTGCTCCTGTGACATCCCCGCCATCTGGAAA
AGCATCTTCCTGTGGCTTGGCTACTCCAACTCCTTCTTTAACCCCCTGATCTATACGGCT
TTCAACAAGAACTACAACAGCGCCTTCAAGAACTTCTTTTCTAGGCAACACTGA

Clone variation with respect to NM_024012.2
12 t=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024012 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCACTTTCCAGAAACT
CCCCCACTGGCCAGAGGTTGCAAACATCCGGATTGGCTCTGGGCACAGTGGCCGCCTTAA
GTCCTCCTGAACACCCCTTCTGCAAGTACCCCAGGGCGGTCTCCTGACCCAGAGATGGAT
TTACCAGTGAACCTAACCTCCTTTTCCCTCTCCACCCCCTCCCCTTTGGAGACCAACCAC
AGCCTCGGCAAAGACGACCTGCGCCCCAGCTCGCCCCTGCTCTCGGTCTTCGGAGTGCTT
ATTCTCACCTTGCTGGGCTTTCTGGTGGCGGCGACGTTCGCCTGGAACCTGCTGGTGCTG
GCGACCATCCTCCGTGTACGCACCTTCCACCGCGTGCCCCACAACCTGGTGGCATCCATG
GCCGTCTCGGATGTCCTGGTGGCCGCGCTGGTCATGCCGCTGAGCCTGGTGCACGAGCTG
TCCGGGCGCCGCTGGCAGCTAGGTCGGAAGCTGTGCCAGCTTTGGATCGCGTGCGACGTG
CTTTGCTGCACGGCCAGCATCTGGAACGTGACGGGCATAGCCCTGGACCGCTACTGGTCC
ATCACGCGCCACTGGAATACACGCTCCGCACCGCAGGTGCGTCTCCAACGTCATGATCGC
GCTCACCTGGGCACTCTTCGCTGTCATCTCTCTGGCCCCGCTGCTTTTTGGCTGGGAAGA
GAGTACTCTGAGGCAGCGAGGAGGCCAGATAACNCGCGAGCCTTCTAGCCCGGTCTTCAC
GTAAGCGCTTCTACTGCGCTTGGGTGGGCTCTTCGTGACCGAAAGACACAGGCTGGCAGT
TCCGCGTGGCTCAGGAGACAATAACGTCTACCATACCAACTGGGGAGGGAGGACTTGCCA
AACCCAAAGGGTTACGGTCGCCGGCACGNCCTTC
3' Read Nucleotide Sequence
>Forward primer walk for NM_024012 unedited
CTCTGCTGGTACCCTTNTTTCNACCGACTCACATCCCCTCTGCTCCTGGACTCCCCGCCT
CTGGAAAGCATCTTCCTGTGGCTTGGCTACTCCAACTCCTTCTTTAACCCCCTGATCTAT
ACGGCTTTCAACAAGAACTACAACAGCGCCTTCAAGAACTTCTTTTCTAGGCAACACTGA
GGGAGAGGACCAGGATTGAAAAAAGTTTCTTCCCATAATTCAGTGGAATTCCCAGTTCAT
CCATTTCCCATCCCCACCCAACAGCCATGTGGACGGGATGAATCCTCACCATTCTCCAGG
GTCATCTTCAGAACTGCACTGGCCGCTTTTCCACCTCCTCAGTAGGAATATGACTCCTCA
TAGAGTTTTGGTGACATGATGTATCTAACTTATCTACTATCCCTTTCTCTGTGCTGACAG
TCATGGTCTTTGCCCGCAAAGTGTCCTTTCCTCCCCAAATTCACTCTGGCATGGTGATCG
ACATTGTCTTAAAGAAGTCAAGGCAAATAAGAAGGAGGAGGTAAAACAATATGGGCAGGT
TGAGATTGAATAGAAAAGAATGAACAAGTCATGATGTAGACCTCTGAGGGTAGGAGACCA
TAGTTCCAGGCTTTCTGGCACAGTCCCCATTTCAAATATTCTCCTCTGTGGTCCTAGTAC
ATATATCCTAATACCGATGGNGTATGACCCAAGAGTTGACCACAAAACACTGTCTCCAAA
TATATTAAAGTATATAGTCCTATCCATGAGAGCAGGGAATCTTAGCTTTAAGGNTTTGTT
TGTGGGCACCCACACCGACATACACCACACACACACACACTATGAAGAAGCAGGGTGTCT
AGCTAGCTAGNTTACTCCAAAATGCATCCCGTGACATGAACAGCACTGAGGNNNTACTAC
TNCTTTCATTNT
Restriction Sites NotI-NotI |
ACCN NM_024012
Insert Size 4250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024012.1, NP_076917.1
RefSeq Size 1074 bp
RefSeq ORF 1074 bp
Locus ID 3361
UniProt ID P47898
Cytogenetics 7q36.2
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
Summary The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. provided by RefSeq, Jul 2008
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Resources
"NM_024012" in other vectors (6)
SKU Description Size Price
RC211300 HTR5A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A) 10 ug
$457.00
RC211300L1 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), Myc-DDK-tagged 10 ug
$757.00
RC211300L2 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), mGFP tagged 10 ug
$757.00
RC211300L3 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), Myc-DDK-tagged 10 ug
$757.00
RC211300L4 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), mGFP tagged 10 ug
$757.00
RG211300 HTR5A (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A) 10 ug
$489.00 $657.00

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