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TNIP2 (NM_024309) Human Untagged Clone

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SKU
SC112274
TNIP2 (untagged)-Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TNIP2
Synonyms ABIN2; FLIP1; KLIP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112274 sequence for NM_024309 edited (data generated by NextGen Sequencing)
ATGTCCCGGGACCCGGGGTCGGGCGGCTGGGAGGAGGCCCCGCGCGCAGCTGCCGCGCTC
TGCACCCTGTACCACGAGGCCGGACAGCGGCTGCGCCGCCTGCAGGACCAGCTCGCTGCC
CGCGACGCCCTCATCGCTCGCCTCCGCGCCCGCCTGGCTGCGCTGGAGGGGGACGCCGCG
CCGTCCCTAGTGGACGCGCTGCTGGAGCAGGTTGCGCGCTTCCGGGAGCAGCTGCGAAGG
CAGGAGGGCGGCGCCGCCGAGGCCCAGATGCGCCAGGAAATTGAGAGGCTGACTGAGCGA
CTAGAAGAAAAAGAGAGGGAGATGCAGCAGCTGCTGAGCCAGCCCCAACACGAGCGAGAG
AAGGAAGTCGTCCTGCTACGGAGGAGCATGGCAGAAGGGGAGCGCGCCCGGGCCGCCAGT
GACGTCCTGTGCCGCTCCTTGGCCAACGAGACCCATCAGCTGCGGAGGACGCTGACCGCC
ACCGCCCACATGTGTCAGCATCTGGCCAAGTGTCTGGATGAACGACAGCATGCACAAAGG
AATGTGGGGGAGAGAAGTCCTGACCAGTCGGAACACACAGATGGGCACACCTCTGTCCAG
AGTGTTATTGAGAAGTTGCAGGAAGAAAATCGACTGTTAAAACAGAAGGTGACTCACGTT
GAAGACCTCAATGCCAAGTGGCAGCGCTACAACGCCAGCAGGGACGAATACGTGAGGGGG
CTCCATGCGCAGCTCAGGGGGCTGCAGATCCCCCACGAGCCCGAGCTGATGAGGAAGGAG
ATCTCCCGGCTCAACAGACAGTTGGAAGAGAAAATAAATGACTGTGCCGAAGTGAAGCAG
GAGCTGGCAGCCTCCAGGACGGCCCGGGATGCTGCGTTGGAGCGGGTGCAGATGCTGGAA
CAGCAGATTCTCGCTTACAAGGATGACTTCATGTCAGAAAGGGCCGATCGGGAACGGGCT
CAAAGTAGGATTCAAGAACTGGAGGAAAAGGTCGCCTCTTTGCTGCACCAGGTGTCCTGG
AGACAGGATTCTCGAGAGCCAGACGCCGGCCGGATTCACGCTGGGAGCAAAACTGCCAAG
TATTTGGCCGCCGACGCATTAGAGCTTATGGTGCCTGGTGGCTGGAGGCCTGGGACTGGG
TCCCAGCAGCCAGAACCCCCTGCAGAGGGCGGGCATCCTGGCGCGGCCCAGAGAGGCCAG
GGGGACCTTCAGTGCCCTCACTGCCTGCAGTGCTTCAGTGACGAGCAAGGGGAAGAGCTC
CTCAGGCATGTGGCCGAGTGCTGCCAGTGA

Clone variation with respect to NM_024309.3
159 c=>t;849 g=>a;1275 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024309 unedited
TCAAATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCGGCGGC
CGCGCGGCCTCCCGGGCGGGCCTACAGCCATGTCCCGGGACCCGGGGTCGGGCGGCTGGG
AGGAGGCCCCGCGCGCAGCTGCCGCGCTCTGCACCCTGTACCACGAGGCCGGACAGCGGC
TGCGCCGCCTGCAGGACCAGCTCGCTGCCCGCGACGCCCTCATCGCTCGCCTCCGCGCCC
GCCTGGCTGCGCTGGAGGGGGACGCCGCGCCGTCCCTAGTGGACGCGCTGCTGGAGCAGG
TTGCGCGCTTCCGGGAGCAGCTGCGAAGGCAGGAGGGCGGCGCCGCCGAGGCCCAGATGC
GCCAGGAAATTGAGAGGCTGACTGAGCGACTAGAAGAAAAAGAGAGGGAGATGCAGCAGC
TGCTGAGCCAGCCCCAACACGAGCGAGAGAAGGAAGTCGTCCTGCTACGGAGGAGCATGG
CAGAAGGGGAGCGCGCCCGGGCCGCCAGTGACGTCCTGTGCCGCTCCTTGGCCAACGAGA
CCCATCAGCTGCGGAGGACGCTGACCGCCACCGCCCACATGTGTCAGCATCTGGCCAAGT
GTCTGGATGAACGACAGCATGCACAAAGGAATGTGGGGGAGAGAAGTCCTGACCAGTCGG
AACACACAGATGGGCACACCTCTGTCCAGAGTGTTATTGAGAAGTTGCAGGAAGAAAATC
GACTGTTAAAACAGAAAGTGACTCACGTTGAAGACCTCATGCCAAGTGGCAGCGCTACAA
CGCCAGCAGGGACGAATACGTGAGGGGGCTCCATGCGCAGCTCAGGNGGCTGCAGATCCC
CACGAGCCCGAGCTGATGAGAAGGAGATCTCCNNGCTCACAGACAGTTGGAAGAGAAAAT
AATGACTGTGCCGAANTGAAGCAGACTGGCAGCTCCAGACGNCCGGNTGCTGCGTTGNAC
GGTGCANAGCTGAACAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024309 unedited
CGAGTTTTTATTCTTTTTTTTTTTTTTTTTTTCCCATCAGCAAGAACGTTTATTATAAAA
ATAGGTGGAATAAAGTAAGTGTGCTTTAACTTCCGGCTGGATGACAAATGCTACTGAAAT
GCAGTTTCTCAGTGCAAACATGTGGCATGCAATGGCGGGGGTGGCCACCCTAGTGTGACG
TGCAGCCGCAATACTCCATTTGACTGGTTCGAGGCAAAAAGGCGACGCTTTATTCCATAA
AACAGGATGGGGCTCCCACCCTGGGGACCATACAAGTGACTCCCTCAGGCAGCCACCCCT
TTCTAGGCAGGCTGGGGGAGGGGCTGGCACACCAGCACCAGATAGCTCTGGCTTAAGCCT
GATGGAGACACAGACTGGGACCTCCCTCTGCCAGATGTTCCTGACCCCATCTCCACCTCC
AGCCTCCAGCCTCACTGGCAGTTCCCTGTGACCCAGCCTGTTCCATAGCCAAAGGGACCA
AAGTGAACGATCAGAGTGCCCCGCAACTATTCTAGGGGCCTTGGCTCTCAGTAGAGCTCA
ACCCATGGCATCTGAGAGCACCCACCCTGTCCCTGAGGGCAGCTGCACCGGGCCAGGAGG
CCGCAAGGGCACGGGTGAGTCTCGGTCACTGGCAGCACTCGGCCACATGCCTGAGGAGCT
CTTCCCCTTGCTCGTCACTGAAGCACTGCAGGCAGTGAGGGCACTGAAGGTCCCCCTGGC
CTCTCTGGGCCGCGCCAGGATGCCCGCCCTCTGCAGGGGGTTCTGGCTGCTGGGACCCAG
TCCCAGGCCTCCAGCCACCAGGCACCATAAGCTCTAATGCGTCGGCCGCCAAATACTTGG
CAGTTTTGCTCCCACGTGAATCCGGCCGGCGTCTGGCTCTCGAGAATCCTGTCTCCAGGA
ACCTGGTGCAGCAAAGAGCCG
Restriction Sites NotI-NotI |
ACCN NM_024309
Insert Size 2250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024309.2, NP_077285.2
RefSeq Size 1951 bp
RefSeq ORF 1290 bp
Locus ID 79155
UniProt ID Q8NFZ5
Cytogenetics 4p16.3
Summary This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.provided by RefSeq, May 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_024309" in other vectors (6)
SKU Description Size Price
RC201339 TNIP2 (Myc-DDK-tagged)-Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1 10 ug
$457.00
RC201339L1 Lenti ORF clone of Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201339L2 Lenti ORF clone of Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201339L3 Lenti ORF clone of Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201339L4 Lenti ORF clone of Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1, mGFP tagged 10 ug
$757.00
RG201339 TNIP2 (tGFP-tagged) - Human TNFAIP3 interacting protein 2 (TNIP2), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines
Charlaftis, Suddason, Wu et al
EMBO J. (2014)
Cited by 48
AI Summary: OriGene contributed pCMV6-XL4 TRAF2, pCMV6-XL4 TNIP2, and pCMV6-Myc TNIP1 plasmids used in this study to investigate the molecular mechanisms of MEKK1 PHD-mediated MAPK activation.
Snippet: … Mouse anti-TRAF2 (sc-7346), rabbit anti-STAM (sc-33588), goat anti-TAB1 (sc-6052), rabbit anti-MEKK1 (sc-252) and rabbit anti-Abin1 (or TNIP1) (sc-134660) antibodies were from Santa Cruz Biotechnology. …Rabbit anti-pItch (AB10050) antibody was purchased from Merck Millipore. …pCMV-Myc mouse MEKK1 and pCMV-Myc mouse MEKK1 C438A, I440A plasmids were purchased from GenScript. pCMV6-XL4 TRAF2, pCMV6-XL4 TNIP2 and pCMV6-Myc TNIP1 were from Origene. … Rabbit anti-pMEKK1 was custom made by ThermoScientific. Rabbit anti-phospho MEKK1 was generated as previously described (Matsuzawa et al, 2008). … Chemical inhibitors include AG-490 (EGFR inhibitor, Santa Cruz Biotechnology), NSC697923 (UBE2N inhibitor, Millipore), (5Z)-7-Oxozeaenol (TAK1 inhibitor, Sigma), SB431542 (TGFβR inhibitor, Sigma) and SB203580 (p38 MAPK inhibitor, Sigma). …
WB FC IP Array
Showing 1-1 of 1 papers.
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