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Apolipoprotein O (APOO) (NM_024122) Human Untagged Clone

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SKU
SC112255
APOO (untagged)-Human apolipoprotein O (APOO), transcript variant 1
  $450.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Apolipoprotein O
Synonyms FAM121B; Mic23; MIC26; MICOS26; My025
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112255 sequence for NM_024122 edited (data generated by NextGen Sequencing)
ATGTTCAAGGTAATTCAGAGGTCCGTGGGGCCAGCCAGCCTGAGCTTGCTCACCTTCAAA
GTCTATGCAGCACCAAAAAAGGACTCACCTCCCAAAAATTCCGTGAAGGTTGATGAGCTT
TCACTCTACTCAGTTCCTGAGGGTCAATCGAAGTATGTGGAGGAGGCAAGGAGCCAGCTT
GAAGAAAGCATCTCACAGCTCCGACACTATTGCGAGCCATACACAACCTGGTGTCAGGAA
ACGTACTCCCAAACTAAGCCCAAGATGCAAAGTTTGGTTCAATGGGGGTTAGACAGCTAT
GACTATCTCCAAAATGCACCTCCTGGATTTTTTCCGAGACTTGGTGTTATTGGTTTTGCT
GGCCTTATTGGACTCCTTTTGGCTAGAGGTTCAAAAATAAAGAAGCTAGTGTATCCGCCT
GGTTTCATGGGATTAGCTGCCTCCCTCTATTATCCACAACAAGCCATCGTGTTTGCCCAG
GTCAGTGGGGAGAGATTATATGACTGGGGTTTACGAGGATATATAGTCATAGAAGATTTG
TGGAAGGAGAACTTTCAAAAGCCAGGAAATGTGAAGAATTCACCTGGAACTAAGTAG

Clone variation with respect to NM_024122.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024122 unedited
CGTCAGATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGCTGATTGAA
CTAAACCTTCGCCGCACCGAGTTTGCAGTACGGCCGTCACCCGCACCGCTGCCTGCTTGC
GGTTGGAGAAATCAAGGCCCTACCGGGCCTCCGTAGTCACCTCTCTATAGTGGGCGTGGC
CGAGGCCGGGGTGACCCTGCCGGAGCCTCCGCTGCCAGCGACATGTTCAAGGTAATTCAG
AGGTCCGTGGGGCCAGCCAGCCTGAGCTTGCTCACCTTCAAAGTCTATGCAGCACCAAAA
AAGGACTCACCTCCCAAAAATTCCGTGAAGGTTGATGAGCTTTCACTCTACTCAGTTCCT
GAGGGTCAATCGAAGTATGTGGAGGAGGCAAGGAGCCAGCTTGAAGAAAGCATCTCACAG
CTCCGACACTATTGCGAGCCATACACAACCTGGTGTCAGGAAACGTACTCCCAAACTAAG
CCCAAGATGCAAAGTTTGGTTCAATGGGGGTTAGACAGCTATGACTATCTCCAAAATGCA
CCTCCTGGATTTTTTCCGAGACTTGGTGTTATTGGTTTTGCTGGCCTTATTGGACTCCTT
TTGGCTAGAGGTTCAAAAATAAAGAAGCTAGTGTATCCGCCTGGTTTCATGGGATTAGCT
GCCTCCCTCTATTATCCACAACAAGCCATCGTGTTTGCCCAGGTCAGTGGGGAGAGATTA
TATGACTGGGGTTTACGAGGATATATAGTCATAGAAGATTTGTGGCAGGAGAACTTTCAA
AGTCAGNACATGTGAAGAATTCACCTGGACTAAGTAGAAACTCCATGCTCTGCCATCTTA
ATCAGGAAAAACTAGACCAGACCTCTGTTATCTTCTGTGAAATCATCCTACAAGCAACTA
AN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024122 unedited
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACTTGGGTTTTAAT
TAGGTACATAAATAGCAACATGAGAATGAGGAGTTCTAAGGCTTGTACATTATCTCTAGG
TGAAGGGATTCCAGGTTAGTTTGCTTGTAGGATGATTTCACAGAAGATAACAGAGGTCTG
GTCTAGTTTTTCCTGATTAAGATGGCAGAGCATGGAGTTTTCTACTTAGTTCCAGGTGAA
TTCTTCACATTTCCTGGCTTTTGAAAGTTCTCCTTCCACAAATCTTCTATGACTATATAT
CCTCGTAAACCCCAGTCATATAATCTCTCCCCACTGACCTGGGCAAACACGATGGCTTGT
TGTGGATAATAGAGGGAGGCAGCTAATCCCATGAAACCAGGCGGATACACTAGCTTCTTT
ATTTTTGAACCTCTAGCCAAAAGGAGTCCAATAAGGCCAGCAAAACCAATAACACCAAGT
CTCGGAAAAAATCCAGGAGGTGCATTTTGGAGATAGTCATAGCTGTCTAACCCCCATTGA
ACCAAACTTTGCATCTTGGGCTTAGTTTGGGAGTACGTTTCCTGACACCAGGTTGTGTAT
GGCTCGCAATAGTGTCGGAGCTGTGAGATGCTTTCTTCAAGCTGGCTCCTTGCCTCCTCC
ACATACTTCGATTGACCCTCAGGAACTGAGTAGAGTGAAAGCTCATCAACCTTCACGGAA
TTTTTGGGAGGTGAGTCCTTTTTTGGTGCTGCATAGACTTTGAAGGTGAGCAAGCTCANG
CTGGCTGGCCCCCACGNACCTCTGAATACCTTTGACATGTCNCTGGCAGCGGNAGGCTCC
GCAGGGTACCCCCGGNCTCGNCACGCCACTTAAGAGGTGATACNGAGGCCCGTTAAGCCC
TTGATTTCTCCACCGCAGCAGCN
Restriction Sites NotI-NotI |
ACCN NM_024122
Insert Size 1020 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024122.2, NP_077027.1
RefSeq Size 1124 bp
RefSeq ORF 597 bp
Locus ID 79135
UniProt ID Q9BUR5
Cytogenetics Xp22.11
Protein Families Secreted Protein, Transmembrane
Summary This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.provided by RefSeq, Sep 2009
Transcript Variant: This variant (1) represents the longer transcript and is predicted to encode the functional protein.
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Documents
Resources
"NM_024122" in other vectors (4)
SKU Description Size Price
RC201451 APOO (Myc-DDK-tagged)-Human apolipoprotein O (APOO), transcript variant 1 10 ug
$450.00
RC201451L3 Lenti ORF clone of Human apolipoprotein O (APOO), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC201451L4 Lenti ORF clone of Human apolipoprotein O (APOO), transcript variant 1, mGFP tagged 10 ug
$750.00
RG201451 APOO (tGFP-tagged) - Human apolipoprotein O (APOO), transcript variant 1 10 ug
$650.00

file-alt Citations

Overexpression of apolipoprotein O does not impact on plasma HDL levels or functionality in human apolipoprotein A-I transgenic mice
Nijstad, de Boer, Lagor et al
Biochim. Biophys. Acta (2011) 1811 (4), 294-9 DOI: 10.1016/j.bbalip.2011.01.008
Cited by 34
AI Summary: OriGene contributed to this study by providing the human ApoO cDNA expression vector (catalog number SC112255), which was used to generate adeno-associated viral vectors for apoO overexpression in mice.
Snippet: 2.3. Generation of adeno-associated viral vectors. To generate adeno-associated viral vectors, an expression vector encoding the human ApoO cDNA was obtained from Origene (catalog number SC112255, Genbank NM_024122).
Showing 1-1 of 1 papers.
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