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SRD5A3 (NM_024592) Human Untagged Clone

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SKU
SC112141
SRD5A3 (untagged)-Human steroid 5 alpha-reductase 3 (SRD5A3)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SRD5A3
Synonyms CDG1P; CDG1Q; KRIZI; SRD5A2L; SRD5A2L1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112141 sequence for NM_024592 edited (data generated by NextGen Sequencing)
ATGGCTCCCTGGGCGGAGGCCGAGCACTCGGCGCTGAACCCGCTGCGCGCGGTGTGGCTC
ACGCTGACCGCCGCCTTCCTGCTGACCCTACTGCTGCAGCTCCTGCCGCCCGGCCTGCTC
CCGGGCTGCGCGATCTTCCAGGACCTGATCCGCTATGGGAAAACCAAGTGTGGGGAGCCG
TCGCGCCCCGCCGCCTGCCGAGCCTTTGATGTCCCCAAGAGATATTTTTCCCACTTTTAT
ATCATCTCAGTGCTGTGGAATGGCTTCCTGCTTTGGTGCCTTACTCAATCTCTGTTCCTG
GGAGCACCTTTTCCAAGCTGGCTTCATGGTTTGCTCAGAATTCTCGGGGCGGCACAGTTC
CAGGGAGGGGAGCTGGCACTGTCTGCATTCTTAGTGCTAGTATTTCTGTGGCTGCACAGC
TTACGAAGACTCTTCGAGTGCCTCTACGTCAGTGTCTTCTCCAATGTCATGATTCACGTC
GTGCAGTACTGTTTTGGACTTGTCTATTATGTCCTTGTTGGCCTAACTGTGCTGAGCCAA
GTGCCAATGGATGGCAGGAATGCCTACATAACAGGGAAAAATCTATTGATGCAAGCACGG
TGGTTCCATATTCTTGGGATGATGATGTTCATCTGGTCATCTGCCCATCAGTATAAGTGC
CATGTTATTCTCGGCAATCTCAGGAAAAATAAAGCAGGAGTGGTCATTCACTGTAACCAC
AGGATCCCATTTGGAGACTGGTTTGAATATGTTTCTTCCCCTAACTACTTAGCAGAGCTG
ATGATCTACGTTTCCATGGCCGTCACCTTTGGGTTCCACAACTTAACTTGGTGGCTAGTG
GTGACAAATGTCTTCTTTAATCAGGCCCTGTCTGCCTTTCTCAGCCACCAATTCTACAAA
AGCAAATTTGTCTCTTACCCGAAGCATAGGAAAGCTTTCCTACCATTTTTGTTTTAA

Clone variation with respect to NM_024592.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024592 unedited
GTAACGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGGGCTA
GCGGGCGGTGGGGGCGCCAGCAGCGCGGAAGGCGGGCACGCGGGCCATGGCTCCCTGGGC
GGAGGCCGAGCACTCGGCGCTGAACCCGCTGCGCGCGGTGTGGCTCACGCTGACCGCCGC
CTTCCTGCTGACCCTACTGCTGCAGCTCCTGCCGCCCGGCCTGCTCCCGGGCTGCGCGAT
CTTCCAGGACCTGATCCGCTATGGGAAAACCAAGTGTGGGGAGCCGTCGCGCCCCGCCGC
CTGCCGAGCCTTTGATGTCCCCAAGAGATATTTTTCCCACTTTTATATCATCTCAGTGCT
GTGGAATGGCTTCCTGCTTTGGTGCCTTACTCAATCTCTGTTCCTGGGAGCACCTTTTCC
AAGCTGGCTTCATGGTTTGCTCAGAATTCTCGGGGCGGCACAGTTCCAGGGAGGGGAGCT
GGCACTGTCTGCATTCTTAGTGCTAGTATTTCTGTGGCTGCACAGCTTACGAAGACTCTT
CGAGTGCCTCTACGTCAGTGTCTTCTCCAATGTCATGATTCACGTCGTGCAGTACTGTTT
TGGACTTGTCTATTATGTCCTTGTTGGCCTAACTGTGCTGAGCCAAGTGCCAATGGATGG
CAGGAATGCCTACATAACAGGGAAAAATCTATTGATGCAAGCACGGTNGGTTCCATATTC
TTGGGATGATGATGTTCATCTGGTCATCTGCCCATCAGTATAAGTGCCATGTTATTCTCG
GCAATCTCAGGGAAAATAAGCCAGAGTGGTCATTCACTGGTACCACAGGATCCCATTTNG
NAGACTGGNTTTGATATGTTTTCTTNCCCTNACTACTTAGCAGAGCTGATGATCTACGTT
TCCATGGCCGTCACCTA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024592 unedited
GGCACGCAATTCTAAATCGAGTTTTTTTTTTTTTTTTTTGCAGATTTGACATTATTATTT
ATTCTTTGAAGATACTTTGGTAGATTCATTTTCTTGAGTGGCACTGCCATGCTCATTCAT
AGAAAACTTGTGGGGTATAGAAATGGAATGGAGAGTTTCAAACAGCTTTGCTGAAACTGT
ACTTTGGGCTCCAGACTCTCCTGTCCTTATGCATTGAAACCATCACCTGGCTTGCATTCT
TAATGACTGAGGTTAACTTAAAACAAACATGGCAGGAAAGCTTTCCTATGCTTCGGGTAA
GAGACAAATTTGCTTTTGTACAATTGGCGGCTGACAAAGGCACACAGGCACTGCTTAAAC
AACACATTTGCCACCACTATCCACCCAAGTTAACCCGTGGAACCCACAGGTGACGGCCAT
GGAAACGCACATCATCACCTCTGCTAAGCACCTAGGGGAAGAAACATATTCTAACCAATC
TCCAAATGGGATCCTGTGGCCACCGTGAATGACCACTCCCCGTTTTATTTTCCCCTGAGA
TTGACGACAAATACCCTGGCCCTTATCCCTGTGGGCATATGACCACATGAACCTCATCCT
TGCAAGAATATGGGCACCACCGCGCTTGCATAAATACATTTACATCTGTTATGAAAGCAA
TACCTGACATTACCATTTGGCACCTTGTCTCAAGTCAGTTTGGCCCCCACGGACCTATTC
GACAAGTCCAAATCAGCACCTGCGCGAAGGTGAATCCATGACATCGTGAGAAAAACCCTT
GACGTACACGCCCCTCCCAAAAGTCTTTCGCTAGTCCGCGCCACCCACACACAATACTTA
CCACCTAACAATGCCCCACTGGGCCCACATACATCTTCCCTTGAAACTGGGGCCGCATCT
CGAAAATTCTGTGACCACCCCCTGATACCACCCCTGGTAAAAGCGGCCTCCCACGGACCC
ACAATTGGGCCACGCGCCCAAGTTGGAATTCCTTTCCAGGCCTCGAAAGATTAAAAGCGG
CTACAAATTCTCTGGCGACACTAN
Restriction Sites NotI-NotI |
ACCN NM_024592
Insert Size 1250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024592.1, NP_078868.1
RefSeq Size 2271 bp
RefSeq ORF 957 bp
Locus ID 79644
UniProt ID Q9H8P0
Cytogenetics 4q12
Domains Steroid_dh
Protein Families Transmembrane
Protein Pathways Androgen and estrogen metabolism
Summary The protein encoded by this gene belongs to the steroid 5-alpha reductase family, and polyprenol reductase subfamily. It is involved in the production of androgen 5-alpha-dihydrotestosterone (DHT) from testosterone, and maintenance of the androgen-androgen receptor activation pathway. This protein is also necessary for the conversion of polyprenol into dolichol, which is required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-linked glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type Iq. provided by RefSeq, Mar 2011
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Documents
Resources
"NM_024592" in other vectors (6)
SKU Description Size Price
RC200970 SRD5A3 (Myc-DDK-tagged)-Human steroid 5 alpha-reductase 3 (SRD5A3) 10 ug
$450.00
RC200970L1 Lenti ORF clone of Human steroid 5 alpha-reductase 3 (SRD5A3), Myc-DDK-tagged 10 ug
$750.00
RC200970L2 Lenti ORF clone of Human steroid 5 alpha-reductase 3 (SRD5A3), mGFP tagged 10 ug
$750.00
RC200970L3 Lenti ORF clone of Human steroid 5 alpha-reductase 3 (SRD5A3), Myc-DDK-tagged 10 ug
$750.00
RC200970L4 Lenti ORF clone of Human steroid 5 alpha-reductase 3 (SRD5A3), mGFP tagged 10 ug
$750.00
RG200970 SRD5A3 (tGFP-tagged) - Human steroid 5 alpha-reductase 3 (SRD5A3) 10 ug
$650.00

file-alt Citations

Hamster SRD5A3 lacks steroid 5α-reductase activity in vitro
Chávez, Ramos, García-Becerra et al
Steroids (2014)
Cited by 33
AI Summary: OriGene’s PCMV6-XL4 plasmid containing the human SRD5A2 cDNA (clone SC119922) was used in transfection experiments to assess and compare steroid 5α-reductase activity in cultured cells.
Snippet: 2.6. Steroid 5α-reductase activity in cultured cells. Enzyme activity measurements after transient transfection with pcDNA3.1/hSrd5a3, pcDNA3.1/hSrd5a1, PCMV6-XL4 (containing the human SRD5A2 cDNA;clone SC119922, OriGene Tech. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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