AAGAB (NM_024666) Human Untagged Clone

SKU SC112105

AAGAB (untagged)-Human alpha- and gamma-adaptin binding protein (AAGAB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AAGAB
• Synonyms: KPPP1; p34; PPKP1; PPKP1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024666, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTGCTGGCGTACCCTGTGCGTTAGTCACCAGCTGCTCCTCCGTCTTCTCAGGAGACCAGCTGGTCC; AACATATCCTTGGAACAGAAGATCTTATTGTGGAAGTGACTTCCAATGATGCTGTGAGATTTTATCCCTG; GACCATTGATAATAAATACTATTCAGCAGACATCAATCTATGTGTGGTGCCAAACAAATTTCTTGTTACT; GCAGAGATTGCAGAATCTGTCCAAGCATTTGTGGTTTACTTTGACAGCACACAAAAATCGGGCCTTGATA; GTGTCTCCTCATGGCTTCCACTGGCAAAAGCATGGTTACCTGAGGTGATGATCTTGGTCTGCGATAGAGT; GTCTGAAGATGGTATAAACCGACAAAAAGCTCAAGAATGGTGCATCAAACATGGCTTTGAATTGGTAGAA; CTTAGTCCAGAGGAGTTGCCTGAGGAGGATGATGACTTCCCAGAATCTACAGGAGTAAAGCGAATTGTCC; AAGCCCTGAATGCCAATGTGTGGTCCAATGTAGTGATGAAGAATGATAGGAACCAAGGCTTTAGCCTTCT; CAACTCATTGACTGGAACAAACCATAGCATTGGGTCAGCAGATCCCTGTCACCCAGAGCAACCCCATTTG; CCAGCAGCAGATAGTACTGAATCCCTCTCTGATCATCGGGGTGGTGCATCTAACACAACAGATGCCCAGG; TTGATAGCATTGTGGATCCCATGTTAGATCTGGATATTCAAGAATTAGCCAGTCTTACCACTGGAGGAGG; AGATGTGGAGAATTTTGAAAGACTCTTTTCAAAGTTAAAGGAAATGAAAGACAAGGCTGCGACGCTTCCT; CATGAGCAAAGAAAAGTGCATGCAGAAAAGGTGGCCAAAGCATTCTGGATGGCAATCGGGGGAGACAGAG; ATGAAATTGAAGGCCTTTCATCTGATGAAGAGCACTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024666 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGAGCGCAGCTATGGCT; GCTGGCGTACCCTGTGCGTTAGTCACCAGCTGCTCCTCCGTCTTCTCAGGAGACCAGCTG; GTCCAACATATCCTTGGAACAGAAGATCTTATTGTGGAAGTGACTTCCAATGATGCTGTG; AGATTTTATCCCTGGACCATTGATAATAAATACTATTCAGCAGACATCAATCTATGTGTG; GTGCCAAACAAATTTCTTGTTACTGCAGAGATTGCAGAATCTGTCCAAGCATTTGTGGTT; TACTTTGACAGCACACAAAAATCGGGCCTTGATAGTGTCTCCTCATGGCTTCCACTGGCA; AAAGCATGGTTACCTGAGGTGATGATCTTGGTCTGCGATAGAGTGTCTGAAGATGGTATA; AACCGACAAAAAGCTCAAGAATGGTGCCTCAAACATGGCTTTGAATTGGTAGAACTTAGT; CCAGAGGAGTTGCCTGAGGAGGATGATGACTTCCCAGAATCTACAGGAGTAAAGCGAATT; GTCCAAGCCCTGAATGCCAATGTGTGGTCCAATGTAGTGATGAAGAATGATAGGAACCAA; GGCTNTAGCCTTCTCAACTCATTGACTGGAACAAACCATAGCATTGGGTCAGCAGATCCC; TGTCACCCAGAGCAACCCCATTTGCCAGCAGCAGATAGTACTGAATCCCTCTCTGATCAT; CGNGGTGGTGCATCTAACACAACAGATGCCCAGGTTGATAGCATTGTGGATCCCATGTTA; GATCTGGNATATTCAAGAATTAGCCCAGTCTTACACTTGGAGNNAGAGATGTGAGAATTT; TGAAGACTCCTTTCAAGTTAAAGAAATGAAAGACAGGCTGCGACGCTTCTNATGAGCAAG; AN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024666 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTACAAAAACAAATAAGGATTTTTATTTGCAGTACTTTCCACTCTTCCTTTAAAAACTT; GCCATTTGCTTATCAGTTCCTCTGGGGCTGACCCACTCAAACAAGACAAAGGATAAAGAA; CAAAAGATAGTCCTCCGAGGTTACAGGCTTGGAAGGGCAAAAAGGAGCTACAAACCTTGG; AAAAAAAACAAGGGGCTCAGGAATTCATCGCCTAACATTTCACTTCCCCACCCACCCCTT; AGTGCTCCCACTTTGGCAGGGATCTCTTTTTGGCTTTAAAGAAAAAGGGGGAAATGTGCC; TTGTTTTGCAGGTGTGCAACAACACAGCTTTGGCATTTCAAGCAGCAGGGGAAAACTCTA; AAACAAAAAAATTTCTTCATGAAAATCACGGTATGTTATCACATACTGTCTCCATGGCCC; ATACAAGGACTCCTTAAGGTTCTCTTTAACATACAACATATCCCCCACAACTCAGTAAAG; AGGTTTTCTTCCCACTGGAATAGAAATCCTTTGCCTCATTTATTACAGTCTAAAAATCCA; CACTGGCTTTTTGATTCTTTCTAGCATGAGCTCAGACACTATACTTCAAAAGAATGCCCA; GAGGCATTTTCTTAGGTAGAGACTTAGCTGCGGGCTGAGACTTCGGCCCCCACATAGCTC; ACATNAATTCTGCAGCTGCCTCCTTTCCTCCAATAAAAGCATCATAGCTCAGAGCTACAA; GCTCCTTTGATGGAAAAAACCCAATCACCCAGGAAAGGTGACACCACTCCTGATGTGCAG; TATCAAGTGTGAATGCTGGAAATGCTACTTNAAACGTTGACCAGAAATGCGCTGNNAGGT; GCCTTCAACTTGAGTAAATACCACAGACTCTGAGATTATCCTACCCTCATCCTAGAACAA; AAAAAGTCATTAAAAATCCTCCTATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_024666
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024666.2, NP_078942.2
• RefSeq Size: 2774 bp
• RefSeq ORF: 948 bp
• Locus ID: 79719
• UniProt ID: Q6PD74
• Cytogenetics: 15q23
• Summary: The protein encoded by this gene interacts with the gamma-adaptin and alpha-adaptin subunits of complexes involved in clathrin-coated vesicle trafficking. Mutations in this gene are associated with type I punctate palmoplantar keratoderma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]; Transcript Variant: This variant (1) encodes the longer isoform (1).