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KEAP1 (NM_012289) Human Untagged Clone

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SKU
SC111946
KEAP1 (untagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2
  $582.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KEAP1
Synonyms INrf2; KLHL19
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_012289, the custom clone sequence may differ by one or more nucleotides


ATGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGTCACAGTGCC
CTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGGAGGTGACGCCCTCCCAGCA
TGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCAAGCAGGCCTTTGGCATCATGAACGAGCTG
CGGCTCAGCCAGCAGCTGTGTGACGTCACACTGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCA
TGGCCCACAAGGTGGTGCTGGCCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGA
GCAGGGCATGGAGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCC
TACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCATGTACCAGA
TCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACCCCAGCAATGCCATCGGCAT
CGCCAACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACCAGCGTGCCCGGGAGTACATCTACATGCAT
TTTGGGGAGGTGGCCAAGCAAGAGGAGTTCTTCAACCTGTCCCACTGCCAACTGGTGACCCTCATCAGCC
GGGACGACCTGAACGTGCGCTGCGAGTCCGAGGTCTTCCACGCCTGCATCAACTGGGTCAAGTACGACTG
CGAACAGCGACGGTTCTACGTCCAGGCGCTGCTGCGGGCCGTGCGCTGCCACTCGTTGACGCCGAACTTC
CTGCAGATGCAGCTGCAGAAGTGCGAGATCCTGCAGTCCGACTCCCGCTGCAAGGACTACCTGGTCAAGA
TCTTCGAGGAGCTCACCCTGCACAAGCCCACGCAGGTGATGCCCTGCCGGGCGCCCAAGGTGGGCCGCCT
GATCTACACCGCGGGCGGCTACTTCCGACAGTCGCTCAGCTACCTGGAGGCTTACAACCCCAGTGACGGC
ACCTGGCTCCGGTTGGCGGACCTGCAGGTGCCGCGGAGCGGCCTGGCCGGCTGCGTGGTGGGCGGGCTGT
TGTACGCCGTGGGCGGCAGGAACAACTCGCCCGACGGCAACACCGACTCCAGCGCCCTGGACTGTTACAA
CCCCATGACCAATCAGTGGTCGCCCTGCGCCCCCATGAGCGTGCCCCGTAACCGCATCGGGGTGGGGGTC
ATCGATGGCCACATCTATGCCGTCGGCGGCTCCCACGGCTGCATCCACCACAACAGTGTGGAGAGGTATG
AGCCAGAGCGGGATGAGTGGCACTTGGTGGCCCCAATGCTGACACGAAGGATCGGGGTGGGCGTGGCTGT
CCTCAATCGTCTCCTTTATGCCGTGGGGGGCTTTGACGGGACAAACCGCCTTAATTCAGCTGAGTGTTAC
TACCCAGAGAGGAACGAGTGGCGAATGATCACAGCAATGAACACCATCCGAAGCGGGGCAGGCGTCTGCG
TCCTGCACAACTGTATCTATGCTGCTGGGGGCTATGATGGTCAGGACCAGCTGAACAGCGTGGAGCGCTA
CGATGTGGAAACAGAGACGTGGACTTTCGTAGCCCCCATGAAGCACCGGCGAAGTGCCCTGGGGATCACT
GTCCACCAGGGGAGAATCTACGTCCTTGGAGGCTATGATGGTCACACGTTCCTGGACAGTGTGGAGTGTT
ACGACCCAGATACAGACACCTGGAGCGAGGTGACCCGAATGACATCGGGCCGGAGTGGGGTGGGCGTGGC
TGTCACCATGGAGCCCTGCCGGAAGCAGATTGACCAGCAGAACTGTACCTGTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_012289 unedited
TCACTATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCGC
GGCCATGGCGGGGTCCCTGAGTGCCAGAGGTGGTGGTGTTGCTTATCTTCTGGAACCCCA
TGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGT
CACAGTGCCCTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGG
AGGTGACGCCCTCCCAGCATGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCA
AGCAGGCCTTTGGCATCATGAACGAGCTGCGGCTCAGCCAGCAGCTGTGTGACGTCACAC
TGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCATGGCCCACAAGGTGGTGCTGG
CCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGAGCAGGGCATGG
AGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCCT
ACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCA
TGTACCAGATCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACC
CCAGCAATGCCATCGGCATCGCCCACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACC
ACCGTGCCCGGGGAGTACATCTACATGCATTTTGGGGAGGTTGGCCAACCAAGAGGAGTT
CTTCACCCTGTCCCACTGGCCACTGGGTGACCCTCATCAGCCGGGACGACCTGNACGTGC
GCTGCGAGTCCCAAGTCTTCCACGCCCTGCATCAACTGGGTGCAAGNACGACTGCGAACA
GCGACCGTTTTACNTTCAGGCGCTTGCTGCGGGCCCGGCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012289 unedited
GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT
TAGTCTGTTCTTTATTTTCCTTGGAAAATTACAGGGTTACTTATCAACAAAATAACTGTC
CCTCCGGGGGGGAAGTGCCATCCGGTGGCTTCTATGTACAGGCCCATCCCTGGGAACCAC
ATTTCCAAAGAGGGGCCTCCCCCTGAGGCCCCCATTGGCCCCCTCCCAGGTATCCAAGAA
TAAATCACATGGTGACAGCTGCCGGCATGGGTGAGTGGGACCCAAGCCTATTACCCGGCC
AGAGGGTTTGGGGGCCTCTCTCCTGGAAGCCTGCTCTTTCCACACCCCCTTTCCCAGCCA
GGCTGTCTTGGACACTCCCGGGGCTCCGCTGAGGGGCACATGATTCCCGCTTTGGACTTC
TTTTGAGATGTCATTTTAACACTGAGGCATCCTGGCCTCCCTTCCCGGAAAATGGGTTAT
TTGCAGAGCTGGCTTTTCTTTTAATCCCGGTTTTTGTACAAAAACAATGATACTCCCCAT
TGGACTGTATTTTTGCCCAAAAAACAAAAGTGCCTCAACAGGTACAGTTCTGCTGGGCAA
TCTGCTTCCCGGAGGGCTCCATGGTGACAGCCACCCCCACCCCACTCCCGCCCCGATGGC
TTTCCGGTCACCTCGCCCCAAGTGGCTTGATTCTGGGCCGAACCCTCCACACTGGTCCGG
AACCTGTGACCCTCTTAGCCCCAAGGCCTAAATTCTCCCCTGGGGCCAAGGATCCCCAGG
CCCTTTCCGGGCCTTTGGGGCCTCCAAACTCCCGTCTCTTTTCACCACAAACGCCCCCCC
TGTCACCGGGCCCGCCCCCATCCCCCCCCCCTAATACCCCCGGACAGACCAAACCCCTCC
CCTTCCGGAGGGCCCCTGCTCTACCCCCGCACCCCCCCTTTGGGGTAGAACCCCACGAAT
ACGCCGTCGCCG
Restriction Sites NotI-NotI |
ACCN NM_012289
Insert Size 2600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012289.3, NP_036421.2
RefSeq Size 2577 bp
RefSeq ORF 1875 bp
Locus ID 9817
UniProt ID Q14145
Cytogenetics 19p13.2
Domains BTB, Kelch
Protein Families Transcription Factors
Protein Pathways Ubiquitin mediated proteolysis
Summary This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1.
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Documents
Resources
"NM_012289" in other vectors (6)
SKU Description Size Price
RC202513 KEAP1 (Myc-DDK-tagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2 10 ug
$581.00
RC202513L1 Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged 10 ug
$881.00
RC202513L2 Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged 10 ug
$881.00
RC202513L3 Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged 10 ug
$881.00
RC202513L4 Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged 10 ug
$881.00
RG202513 KEAP1 (tGFP-tagged) - Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2 10 ug
$489.00 $781.00

file-alt Citations

N-acetyl-l-cysteine ethyl ester (NACET) induces the transcription factor NRF2 and prevents retinal aging and diabetic retinopathy
Realini, Amato, Rasa et al
Redox Biol (2025) 88, 103914 DOI: 10.1016/j.redox.2025.103914
Cited by 5
AI Summary: OriGene contributed to this study by providing the KEAP1 expression plasmid (Cat# SC111946), which was used to restore KEAP1 expression in KEAP1-silenced cells for mechanistic investigations of NRF2 activation.
Snippet: TRC shRNA library (Sigma-Aldrich;Merck KGaA, Darmstadt, Germany) expressing specific shRNA for 3’ UTR of human KEAP1 (#TRCN0000155340). Plasmid for KEAP1 (NM_012289) expression in KEAP1-silenced cells was purchased from ORIGENE (#SC111946;Rockville, MD, USA)
Rotavirus NSP1 associates with components of the cullin RING ligase family of E3 ubiquitin ligases
Lutz, Pace, Arnold
J. Virol. (2016)
AI Summary: OriGene products, including the plasmids pCMV6-XL5-Cul3, pCMV6-XL5-KEAP1, pCMV6-XL5-KLHL12, and pCMV6-XL5-SPOP, were used to express key cullin RING ligase components, enabling the study of their interactions with rotavirus NSP1 in this research.
Snippet: pCMV6-XL5-Cul3 (Origene), pENTR223-Rbx1 (Dana Farber Harvard Cancer Center PlasmID 181 Page 9. 9 Repository), pCMV6-XL5-KEAP1 (Origene), pCMV6-XL5-KLHL12 (Origene), and pCMV6-XL5- 182 SPOP (Origene). ... Cite SaveSaving
KEAP1 is a redox sensitive target that arbitrates the opposing radiosensitive effects of parthenolide in normal and cancer cells
Xu, Fang, Miriyala et al
Cancer Res. (2013) 73 (14), 4406-17 DOI: 10.1158/0008-5472.CAN-12-4297
AI Summary: OriGene’s plasmid-cloned Keap1 cDNA and Bcl-xL cDNA were used in this study to investigate the roles of KEAP1 and Bcl-xL in mediating the differential radiosensitizing effects of parthenolide in cancer and normal cells.
Snippet: All cell lines were cultured and maintained in the media recommended in the manufacturer's protocols. Plasmid cloned Keap1 cDNA and Bcl-xL cDNA (OriGene) and siRNAs for knocking down Keap1, Nrf2, thioredoxin (TrX), PGAM5, and Bcl-xL (Dharmacon)
Dysfunctional KEAP1-NRF2 interaction in non-small-cell lung cancer
Singh, Misra, Thimmulappa et al
PLoS Med (2006) 3 (10), e420 DOI: 10.1371/journal.pmed.0030420
Cited by 1060
AI Summary: In this study, OriGene’s KEAP1 cDNA clone (OriGene, SC119278) was used to investigate the functional impact of KEAP1 mutations in non-small-cell lung cancer cells.
FigureFigureFigure
Showing 1-4 of 4 papers.
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