NOTUM (NM_178493) Human Untagged Clone

SKU
SC111889
NOTUM (untagged)-Human notum pectinacetylesterase homolog (Drosophila) (NOTUM)
$686.00
5 Weeks*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NOTUM
Synonyms hNOTUM
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_178493, the custom clone sequence may differ by one or more nucleotides


ATGGGCCGAGGGGTGCGCGTGCTGCTGCTGCTGAGCCTGCTGCACTGCGCCGGGGGCAGCGAGGGCAGGA
AGACCTGGCGGCGCCGGGGTCAGCAGCCGCCTCCTCCCCCGCGGACCGAGGCGGCGCCGGCGGCCGGACA
GCCCGTGGAGAGCTTCCCGCTGGACTTCACGGCCGTGGAGGGTAACATGGACAGCTTCATGGCGCAAGTC
AAGAGCCTGGCGCAGTCCCTGTACCCCTGCTCCGCGCAGCAGCTCAACGAGGACCTGCGCCTGCACCTCC
TACTCAACACCTCGGTGACCTGCAACGACGGCAGCCCCGCCGGCTACTACCTGAAGGAGTCCAGGGGCAG
CCGGCGGTGGCTCCTCTTCCTGGAAGGCGGCTGGTACTGCTTCAACCGCGAGAACTGCGACTCCAGATAC
GACACCATGCGGCGCCTCATGAGCTCCCGGGACTGGCCGCGCACTCGCACAGGCACAGGGATCCTGTCCT
CACAGCCGGAGGAGAACCCCTACTGGTGGAACGCAAACATGGTCTTCATCCCCTACTGCTCCAGTGATGT
TTGGAGCGGGGCTTCATCCAAGTCTGAGAAGAACGAGTACGCCTTCATGGGCGCCCTCATCATCCAGGAG
GTGGTGCGGGAGCTTCTGGGCAGAGGGCTGAGCGGGGCCAAGGTGCTGCTGCTGGCCGGGAGCAGCGCGG
GGGGCACCGGGGTGCTCCTGAATGTGGACCGTGTGGCTGAGCAGCTGGAGAAGCTGGGCTACCCAGCCAT
CCAGGTGCGAGGCCTGGCTGACTCCGGCTGGTTCCTGGACAACAAGCAGTATCGCCACACAGACTGCGTC
GACACGATCACGTGCGCGCCCACGGAGGCCATCCGCCGTGGCATCAGGTACTGGAACGGGGTGGTCCCGG
AGCGCTGCCGACGCCAGTTCCAGGAGGGCGAGGAGTGGAACTGCTTCTTTGGCTACAAGGTCTACCCGAC
CCTGCGCTGCCCTGTGTTCGTGGTGCAGTGGCTGTTTGACGAGGCACAGCTGACGGTGGACAACGTGCAC
CTGACGGGGCAGCCGGTGCAGGAGGGCCTGCGGCTGTACATCCAGAACCTCGGCCGCGAGCTGCGCCACA
CACTCAAGGACGTGCCGGCCAGCTTTGCCCCCGCCTGCCTCTCCCATGAGATCATCATCCGGAGCCACTG
GACGGATGTCCAGGTGAAGGGGACGTCGCTGCCCCGAGCACTGCACTGCTGGGACAGGAGCCTCCATGAC
AGCCACAAGGCCAGCAAGACCCCCCTCAAGGGCTGCCCCGTCCACCTGGTGGACAGCTGCCCCTGGCCCC
ACTGCAACCCCTCATGCCCCACCGTCCGAGACCAGTTCACGGGGCAAGAGATGAACGTGGCCCAGTTCCT
CATGCACATGGGCTTCGACATGCAGACGGTGGCCCAGCCGCAGGGACTGGAGCCCAGTGAGCTGCTGGGG
ATGCTGAGCAACGGAAGCTAG


5' Read Nucleotide Sequence
>OriGene 5' read for NM_178493 unedited
NNNNAATGTTCANAATTTTGTNAATACGAACTNCATNATAGGGNNCGGCCGCGNNAATTC
GGCACCAGAANCTCAACGAGGACCTGCGCCTGCACCTCCTACTCAACACCTCGGTGACCT
GCAACGACGGCAGCCCCGCCGGCTACTACCTGAAGGAGTCCAGGGGCAGCCGGCGGTGGC
TCCTCTTCCTGGAAGGCGGCTGGTACTGCTTCAACCGCGAGAACTGCGACTCCAGATACG
ACACCATGCGGCGCCTCATGAGCTCCCGGGACTGGCCGCGCACTCGCACAGGCACAGGGA
TCCTGTCCTCACAGCCGGAGGAGAACCCCTACTGGTGGAACGCAAACATGGTCTTCATCC
CCTACTGCTCCAGTGATGTTTGGAGCGGGGCTTCATCCAAGTCTGAGAAGAACGAGTACG
CCTTCATGGGCGCCCTCATCATCCAGGAGGTGGTGCGGGAGCTTCTGGGCAGAGGGCTGA
GCGGGGCCAAGGTGCTGCTGCTGGCCGGGAGCAGCGCGGGGGGCACCGGGGTGCTCCTGA
ATGTGGACCGTGTGGCTGAGCAGCTGGAGAAGCTGGGCTACCCAGCCATCCAGGTGCGAG
GCCTGGCTGACTCCGGCTGGTTCCTGGACAACAAGCAGTATCGCCACACAGACTGCGTCG
ACACGATCACGTGCGCGCCCACGGAGGCCATCCGCCGTGGCATCAGGTACTGGAACGGNG
TGGTCCCGGAGCGCTGCCGACGCCAGTTCCAGGAAGGCGAGGAGTGGAACTGCTTCTTTG
GCTACAAGGTCTACCCGACCCTGCGCTGCCCTGTGTTCGTGGTGCAGTGGCTGTTTGACG
AGCACAGCTGACGGTGGACACGTGCACCTGACGGGGCAGCCGTGCCCAGAGGCCTGCNGC
TGTACATCCAGAACCTCGGCCGCGAGCTGCGCCAACACTCAAGGACGTG
Restriction Sites NotI-NotI
ACCN NM_178493
Insert Size 2000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178493.3, NP_848588.2
RefSeq Size 2281 bp
RefSeq ORF 1293 bp
Locus ID 147111
UniProt ID Q6P988
Cytogenetics 17q25.3
Summary Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.