NOVA1 (NM_002515) Human Untagged Clone
SKU
SC111659
NOVA1 (untagged)-Human neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
$473.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | NOVA1 |
| Synonyms | Nova-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_002515 |
| Insert Size | 3750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002515.2, NP_002506.2 |
| RefSeq Size | 3918 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 4857 |
| UniProt ID | P51513 |
| Cytogenetics | 14q12 |
| Domains | KH |
| Summary | This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) encodes the longest isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002515" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210407 | NOVA1 (Myc-DDK-tagged)-Human neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1 | 10 ug |
$472.00
|
|
| RC210407L3 | Lenti ORF clone of Human neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$772.00
|
|
| RC210407L4 | Lenti ORF clone of Human neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1, mGFP tagged | 10 ug |
$772.00
|
|
| RG210407 | NOVA1 (tGFP-tagged) - Human neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1 | 10 ug |
$489.00
$672.00
|
|
Citations
The splicing machinery is dysregulated and represents a therapeutic vulnerability in breast cancer
Hermán-Sánchez, G-García, Jiménez-Vacas et al
Cell Mol Life Sci (2024) 82 (1), 18 DOI: 10.1007/s00018-024-05515-6
Hermán-Sánchez, G-García, Jiménez-Vacas et al
Cell Mol Life Sci (2024) 82 (1), 18 DOI: 10.1007/s00018-024-05515-6
Cited by 3
AI Summary: OriGene's NOVA1 siRNA (SC111659 C) was used to silence NOVA1 expression in breast cancer cell lines, enabling the study of NOVA1's role in cancer aggressiveness and splicing dysregulation.
Snippet: significant overexpression of NOVA1/PRPF8 among the tested concentrations (500 ng/mL, 1000 ng/mL, and 1500 ng/mL). NOVA1 silencing was performed using one siRNA sequence targeting NOVA1 (SC111659 C, Origene, Rockville, MD, USA) and Lipofectamine RNAiMAX (Thermo Fisher). For pharmacological treatment with pladienolide B (QUIMIGEN, Madrid, Spain), a half maximal inhibitory concentration (IC50) test
Spliceosomic dysregulation unveils NOVA1 as a candidate actionable therapeutic target in pancreatic neuroendocrine tumors
Pedraza-Arevalo, Alors-Pérez, Blázquez-Encinas et al
Transl Res (2022)
Pedraza-Arevalo, Alors-Pérez, Blázquez-Encinas et al
Transl Res (2022)
Cited by 23
AI Summary: OriGene products, specifically the NOVA1 siRNA (SC111659 A and B) and NOVA1 overexpression plasmid (SR303213), were used to manipulate NOVA1 expression in cell models, enabling the study to investigate NOVA1’s role in pancreatic neuroendocrine tumor cell proliferation and signaling.
Snippet: … Inappropriate function of this Save;QGP-1 and BON-1 cells were transfected with specific siRNAs or overexpression plasmid, previously validated in our laboratory (SC111659 A and B, and SR303213, respectively;Origene, Rockville, MD, USA). Specifically, cells were seeded in 6-well culture plates and transfected with NOVA1 siRNA (100 nM) or plasmid (1 µg), using Lipofectamine RNAiM
Physical and Functional Interaction between 5-HT6 Receptor and Nova-1
Kim, Seo, Hwang et al
Exp Neurobiol (2019) 28 (1), 17-29 DOI: 10.5607/en.2019.28.1.17
Kim, Seo, Hwang et al
Exp Neurobiol (2019) 28 (1), 17-29 DOI: 10.5607/en.2019.28.1.17
AI Summary: In this study, the full-length human Nova-1 in pCMV6-XL5 plasmid purchased from OriGene was used to investigate the interaction between 5-HT6 receptor and Nova-1, enabling functional and localization analyses.
Snippet: TC-3′;Rv, 5′-CTCGAGAGTGTCACTGGGCTGAGCAG-3′) and 7BRCT (Fw, 5′-GAATTCCCGGACCTGAGGACCACCTATC-3′;Rv, 5′-CTCGAGCAGCACAAACTCAGGTC- 3′). Full length human Nova-1 in pCMV6-XL5 was purchased from Origene (Rockville Cached
Redundant contribution of a Transient Receptor Potential cation channel Member 1 exon 11 single nucleotide polymorphism to equine congenital stationary night blindness
Scott, John, Bellone et al
BMC Vet. Res. (2016) 12 (1), 121 DOI: 10.1186/s12917-016-0745-1
Scott, John, Bellone et al
BMC Vet. Res. (2016) 12 (1), 121 DOI: 10.1186/s12917-016-0745-1
Cited by 7
AI Summary: OriGene's pCMV6-XL5 expression vector was used to overexpress human Nova-1 in cultured choroidal melanocytes, enabling the study of Nova-1’s effect on TRPM1 mRNA expression in the context of equine congenital stationary night blindness.
Snippet: choroidal melanocytes. Transfection constructs. Nova 1 expression in cultured choroidal melanocytes was increased by transfecting cells with human Nova-1 in Origene's pCMV6-XL5 expression vector. Microphthalmia-associated ... Cite SaveSaving
Showing 1-4 of 4 papers.
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