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CCDC28B (NM_024296) Human Untagged Clone

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SKU
SC111413
CCDC28B (untagged)-Human coiled-coil domain containing 28B (CCDC28B)
  $300.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CCDC28B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_024296, the custom clone sequence may differ by one or more nucleotides


ATGGATGACAAAAAGAAGAAACGGAGTCCCAAGCCCTGCCTGGCCCAGCCAGCCCAGGCCCCAGGCACAC
TACGGAGGGTCCCTGTGCCTACCAGCCACAGCGGCTCCTTGGCCCTAGGACTTCCTCATCTGCCATCCCC
CAAGCAGCGGGCCAAGTTCAAGAGAGTAGGCAAAGAGAAGTGCCGCCCAGTCCTGGCTGGAGGTGGAAGC
GGCTCTGCAGGCACGCCCCTGCAGCACTCCTTCCTGACCGAGGTGACTGATGTCTATGAGATGGAGGGGG
GACTCCTGAACCTGCTCAATGATTTCCACTCTGGCCGGCTGCAGGCCTTCGGGAAGGAATGCTCCTTTGA
GCAGCTGGAGCACGTTCGGGAGATGCAGGAGAAGCTAGCCCGGCTGCACTTCAGCCTGGATGTGTGTGGG
GAGGAGGAGGACGATGAAGAGGAAGAGGATGGGGTCACTGAGGGGCTGCCAGAGGAGCAGAAGAAGACAA
TGGCTGACCGTAACCTGGACCAGCTGCTTAGCAATCTGGAAGACCTCAGTAATTCTATCCAGAAGCTGCA
CCTGGCCGAGAACGCCGAGCCTGAGGAGCAGTCCGCTGCGTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_024296 unedited
CGTCAGCAATTGTAAACGACTCATATAGGCGGCCGCGAAATCGGCACGAGGCCTCGTGCC
GAATTCGGCACGAGGATGGATGACAAAAAGAAGAAACGGAGTCCCAAGCCCTGCCTGGCC
CAGCCAGCCCAGGCCCCAGGCACACTACGGAGGGTCCCTGTGCCTACCAGCCACAGCGGC
TCCTTGGCCCTAGGACTTCCTCATCTGCCATCCCCCAAGCAGCGGGCCAAGTTCAAGAGA
GTAGGCAAAGAGAAGTGCCGCCCAGTCCTGGCTGGAGGTGGAAGCGGCTCTGCAGGCACG
CCCCTGCAGCACTCCTTCCTGACCGAGGTGACTGATGTCTATGAGATGGAGGGGGGACTC
CTGAACCTGCTCAATGATTTCCACTCTGGCCGGCTGCAGGCCTTCGGGAAGGAATGCTCC
TTTGAGCAGCTGGAGCACGTTCGGGAGATGCAGGAGAAGCTAGCCCGGCTGCACTTCAGC
CTGGATGTGTGTGGGGAGGAGGAGGACGATGAAGAGGAAGAGGATGGGGTCACTGAGGGG
CTGCCAGAGGAGCAGAAGAAGACAATGGCTGACCGTAACCTGGACCAGCTGCTTAGCAAT
CTGGAAGACCTCAGTAATTCTATCCAGAAGCTGCACCTGGCCGAGAACGCCGAGCCTGAG
GAGCAGTCCGCTGCGTAGGCGTCCCACGCAGGCCCACACTGCCCCTCTCATTCTCTTCAA
ACTGTGACTNTNTACAGACTCGGGCGGGTGTTCTGCGGCCCCCCAGTGCTATGGGGGCAG
GGGGCGTTGAATGGCATTAACCAGAAAGAAGGCTNNNNNNNNNNNNNCCCNNNNCNNNNN
CNCNNCNNNNNNNCNCCCNCCCCTCCCNNCCCCCCCCCCCCCTCTCCCCCCTTCTCCCTC
TCCCACACTCCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024296 unedited
TGGTAAATCTCCTCTCAACGCCCCCCGTCCCCCATAGCACCTGGGGGGCCGCAGAACACC
CGCCCGAGTCTGTAAAAGTCACAGTTTGAAGAGAATGAGAGGGGCAGTGTGGGCCTGCGT
GGGACGCCTACGCAGCGGACTGCTCCTCAAGCTCGGCGTTCTCGGCCAGGTGCAGCTTCT
GGATAGAATTACTGAGGTCTTCCAGATTGCTAATCAGCTGGTCCAGGTTACGGTCAGCCA
TTGTCTTCTTCTGCTCCTCTGGCAGCCCCTCAGTGACCCCATCCTCTTCCTCTTCATCGT
CCTCCTCCTCCCCACACACATCCAGGCTGAAGTGCAGCCGGGCTAGCTTCTCCTGCATCT
CCCGAACGTGCTCCAGCTGCTCAAAGGAGCATTCCTTCCCGAAGGCCTGCAGCCGGCCAG
AGTGGAAATCATTGAGCAGGTTCAGGAGTCCCCCCTCCATCTCATAGACATCAGTCACCT
CGGTCAAGAAAGAGTGCTGCAGGGGCGTGCCTGCAGAGCCGCTTCCACCTCCAGCCAGGA
CTGAGCGGCACTTCTCTTTGCCTACTCTCTTGAACTTGGCCCGCTGCTTGGGGGATGGCA
GATGATGAAGTCCTATGGCCAAAGAGCCGCTGTGGCTGGTAAGCACAGGGACCCTNCGTA
NTGTGCCTGGGGCCCTGAGCTGGCTGGGCCANGCANGGNCTTGAGACCTCGTTTCTCTCT
TTTTGTCATCCATTCTCGTGCCGAATTCGGCACGAAGGCTCGTGCCGAATTCGCCGCCGC
CCTATAGTGAGTCGGATTTCAAAATNCTGACCGTTCACTAAACGAACTCTGCTTATTTAG
ACTCCCACCGTACACCCCTACCGGCCATTGGGTTAACGGGGCGGGGTTATTACGAACTTT
TGGAAAGTCCCGTTGGTTTTGGTGGCAAACAAACTCCTTTGGCCGCATGGGGTGGAAACT
TGGAATCCCGCGGAGTAACCCCTTTCACCCCCTGGGGGCCGGCAAAACCCTTCCATGTGA
A
Restriction Sites NotI-NotI |
ACCN NM_024296
Insert Size 980 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024296.2, NP_077272.1
RefSeq Size 1448 bp
RefSeq ORF 726 bp
Locus ID 79140
UniProt ID Q9BUN5
Cytogenetics 1p35.2
Summary The product of this gene localizes to centrosomes and basal bodies. The protein colocalizes with several proteins associated with Bardet-Biedl syndrome (BBS) and participates in the regulation of cilia development. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2014
Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.
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Documents
Resources
"NM_024296" in other vectors (4)
SKU Description Size Price
RC203631 CCDC28B (Myc-DDK-tagged)-Human coiled-coil domain containing 28B (CCDC28B) 10 ug
$300.00
RC203631L3 Lenti ORF clone of Human coiled-coil domain containing 28B (CCDC28B), Myc-DDK-tagged 10 ug
$600.00
RC203631L4 Lenti ORF clone of Human coiled-coil domain containing 28B (CCDC28B), mGFP tagged 10 ug
$600.00
RG203631 CCDC28B (tGFP-tagged) - Human coiled-coil domain containing 28B (CCDC28B) 10 ug
$489.00 $500.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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