TMLHE (NM_018196) Human Untagged Clone

SKU SC111322

TMLHE (untagged)-Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMLHE
• Synonyms: AUTSX6; BBOX2; TMLD; TMLH; TMLHED; XAP130
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018196 edited; ATGTGGTACCACAGATTGTCCCACCTACACAGCAGGCTTCAGGACTTGCTGAAGGGAGGA; GTCATATATCCGGCCCTTCCACAGCCCAACTTCAAAAGCTTACTTCCTTTAGCTGTCCAT; TGGCACCATACAGCCTCCAAGTCTCTGACTTGTGCTTGGCAGCAACATGAAGATCATTTT; GAGCTGAAATATGCTAATACCGTGATGCGCTTTGATTACGTCTGGCTTCGAGACCACTGC; CGCTCAGCATCGTGCTACAACTCTAAGACTCACCAGCGCAGCCTGGATACTGCCAGTGTG; GATTTATGTATCAAGCCAAAGACCATTCGTCTGGATGAGACCACACTCTTTTTCACTTGG; CCAGATGGTCATGTGACTAAATATGATTTGAATTGGCTGGTGAAAAACAGCTATGAAGGG; CAGAAACAAAAAGTCATCCAGCCTAGAATACTATGGAATGCTGAAATCTACCAGCAAGCC; CAAGTTCCATCGGTAGATTGCCAGAGCTTCTTAGAAACCAACGAGGGACTGAAGAAGTTT; CTGCAAAACTTTCTGCTCTATGGAATTGCATTCGTAGAAAATGTCCCTCCCACTCAAGAG; CACACAGAGAAGTTGGCAGAAAGGATCAGCTTAATCAGAGAAACCATTTATGGGAGGATG; TGGTATTTCACTTCAGACTTCTCCAGAGGTGACACTGCGTACACCAAGCTAGCTCTGGAT; CGGCACACTGACACTACCTATTTTCAAGAGCCCTGTGGCATTCAAGTGTTTCATTGTCTT; AAACATGAAGGAACTGGTGGCAGGACACTGCTAGTAGATGGATTCTATGCAGCAGAACAG; GTACTTCAAAAGGCACCTGAGGAATTTGAACTCCTCAGTAAAGTGCCATTGAAGCATGAA; TATATTGAAGATGTTGGAGAATGTCACAACCACATGATTGGGATTGGGCCAGTCTTAAAT; ATCTACCCATGGAATAAAGAGCTGTATTTGATCAGGTACAACAACTATGACCGGGCTGTC; ATCAATACCGTTCCTTATGATGTCGTCCATCGCTGGTATACAGCACACCGGACTCTAACG; ATAGAGTTGAGGAGACCTGAGAATGAGTTTTGGGTCAAACTAAAGCCTGGCAGGGTCCTA; TTTATAGACAACTGGCGTGTCCTACATGGCAGGGAATGCTTCACTGGCTACCGCCAACTG; TGTGGCTGCTATTTAACAAGAGATGATGTATTAAACACTGCTCGCCTCTTGGGGCTTCAG; GCTTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018196 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGAGCGGGCTGGGGGAGGG; GAGCGTGGGGCCGACAGTTTTGGGGGTGAAAAGGCAAAAGGCGGGTGAAAGGCTGCCTCC; CGAGACTCTCCTTGCTTGGAATTCTGCCCACTCTGCGGAGTTAGCAGTCACGACCTCCAG; CACAGGATGTGGTACCACAGATTGTCCCACCTACACAGCAGGCTTCAGGACTTGCTGAAG; GGAGGAGTCATATATCCGGCCCTTCCACAGCCCAACTTCAAAAGCTTACTTCCTTTAGCT; GTCCATTGGCACCATACAGCCTCCAAGTCTCTGACTTGTGCTTGGCAGCAACATGAAGAT; CATTTTGAGCTGAAATATGCTAATACCGTGATGCGCTTTGATTACGTCTGGCTTCGAGAC; CACTGCCGCTCAGCATCGTGCTACAACTCTAAGACTCACCAGCGCAGCCTGGATACTGCC; AGTGTGGATTTATGTATCAAGCCAAAGACCATTCGTCTGGATGAGACCACACTCTTTTTC; ACTTGGCCAGATGGTCATGTGACTAAATATGATTTGAATTGGCTGGTGAAAAACAGCTAT; GAAGGGCAGAAACAAAAAGTCATCCAGCCTAGAATACTATGGAATGCTGAAATCTACCAG; CAAGCCCAAGTTCCATCGGTAGATTGCCAGAGCTTCTTAAAAACAACGAGGGACTGAAGA; AATTTCTGCAAAACTTTCTGCTCTATGGAANTGCATTCGTAGAANATGTCCCTCCCACTC; AGACACACAGAGAAGNTGGGCAGAAGGATCAGCTTNATCAGAGAACCATTTATGGGGAGA; TGTGGTATTTCACTTCAGACTCTNCAGAGTGACCTGCGTACACCAGCTAGCTCTGATCGG; CCACTGACCTACTATTTCAGAGCCTGGGCATCAGTGTTCATGGCTAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018196 unedited; GCTATGGACCGCGGCCCGCCTGCTAAATACGGTTAATCCCTTTTTTTTTTTGACTCATTC; AAATTTTGGCAATTAGCATGTATAGTATGCCTATGAGAGAGAGAGAGAGAAATTAGCAGA; AATGGAGGAAAACATATTACATCCTAAATACCACCAAATTGCCAAAATACACATTTGGGT; AGTAATCTCCAGAGAACTCTCTGGAGGCACAGCATCTATCATGCTGATTTCCCTGAACAA; TATTACAGAATAGCCCTGTGTACTTTCTCTTGGGGGGGGGAGGTGTAAAGAATTATCATA; TGGAACCTCACAAGTCTCTAAAACCTTTCTGCAATTATCCCTCATAGGATTACCACACTG; ATGAGTTTGTTCAACAGGCGCTGTTGTGACTTCCTAATTTAACTCCTATAGAGATGATTT; GTGTGGAAGATAAAACACTATATTTGAACTCAATTCCCACCTTATCCCTGAGCTTATGCT; ACCAAGCAATTCTTACAATGTTTGGAGTTCTATTAGACTACAGAGAAGAATCTAGGGTTT; CAGCTAAACCCTTTAATCCACAGGCCTAGGTAAAGGACTGTCTCAAGGTAGTCGCTAGCC; CATGGGTTCTCAAAGTATGGTCTGAAGACCCCTGGAGGAGTCCCTGAGACCCTTTTCGTG; GGTCTGCAGATTAAAAATTATTTTGTATAATAACACCAAGATGAGTTACATTCAATTAGG; CATTTTTCTTTTGAAATTAAGCAGACTTTTGGAAACTAAGATGTGCATAGCCCTGTAGTA; AGCTAAGTGGAAGTTACCAAGGCAGTAGTAGCCTTTTTTTCCCCCTCTCGNTATCTCATG; AAGATACAGAGGACTTTTCCAGAGCTACATGACATATGGTTATGGCATCTCTCTCATAGC; TGACGGATGTGTGCTTCCGCGCTCCCTTCTGAGCTCTTAAAATTTCTCAGTCCTTATACC; CTAAATAATTAGTACTCCAAGATAGGACCCCACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_018196
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018196.1, NP_060666.1
• RefSeq Size: 2836 bp
• RefSeq ORF: 1266 bp
• Locus ID: 55217
• UniProt ID: Q9NVH6
• Cytogenetics: Xq28
• Domains: Gamma-BBH
• Protein Pathways: Lysine degradation
• Summary: This gene encodes the protein trimethyllysine dioxygenase which is the first enzyme in the carnitine biosynthesis pathway. Carnitine play an essential role in the transport of activated fatty acids across the inner mitochondrial membrane. The encoded protein converts trimethyllysine into hydroxytrimethyllysine. A pseudogene of this gene is found on chromosome X. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.