PAICS (NM_006452) Human Untagged Clone

SKU SC111158

PAICS (untagged)-Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PAICS
• Synonyms: ADE2; ADE2H1; AIRC; PAIS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111158 sequence for NM_006452 edited (data generated by NextGen Sequencing); ATGGCGACAGCTGAGGTACTGAACATTGGTAAAAAATTATATGAGGGTAAAACAAAAGAA; GTCTACGAATTGTTAGACAGTCCAGGAAAAGTCCTCCTGCAGTCCAAGGACCAGATTACA; GCAGGAAATGCAGCTAGAAAAAACCACCTGGAAGGAAAAGCTGCAATCTCAAATAAAATC; ACCAGTTGTATTTTTCAGTTATTACAGGAAGCAGGTATTAAAACTGCCTTCACCAGAAAA; TGTGGGGAGACAGCTTTCATTGCACCGCAGTGTGAAATGATTCCAATTGAATGGGTTTGC; AGAAGAATAGCAACTGGTTCTTTTCTCAAAAGAAATCCTGGTGTCAAGGAAGGATATAAG; TTTTACCCACCTAAAGTGGAGTTGTTTTTCAAGGATGATGCCAATAATGACCCACAGTGG; TCTGAGGAACAGCTGATTGCTGCAAAATTTTGCTTTGCTGGACTTCTTATAGGCCAGACT; GAAGTGGATATCATGAGTCATGCTACACAGGCTATATTTGAAATACTGGAGAAATCCTGG; TTGCCCCAGAATTGTACACTGGTTGATATGAAGATTGAATTTGGTGTTGATGTAACCACC; AAAGAAATTGTTCTTGCTGATGTTATTGACAATGATTCCTGGAGACTCTGGCCATCAGGA; GATCGAAGCCAACAGAAAGACAAACAGTCTTATCGGGACCTCAAAGAAGTAACTCCTGAA; GGGCTCCAAATGGTAAAGAAAAACTTTGAGTGGGTTGCAGAGAGAGTAGAGTTGCTTTTG; AAATCAGAAAGTCAGTGCAGGGTTGTAGTGTTGATGGGCTCTACTTCTGATCTTGGTCAC; TGTGAAAAAATCAAGAAGGCCTGTGGAAATTTTGGCATTCCATGTGAACTTCGAGTAACA; TCTGCGCATAAAGGACCAGATGAAACTCTGAGGATTAAAGCTGAGTATGAAGGGGATGGC; ATTCCTACTGTATTTGTGGCAGTGGCAGGCAGAAGTAATGGTTTGGGACCAGTGATGTCT; GGGAACACTGCATATCCAGTTATCAGCTGTCCTCCCCTCACACCAGACTGGGGAGTTCAG; GATGTGTGGTCTTCTCTTCGACTACCCAGTGGTCTTGGCTGTTCAACCGTACTTTCTCCA; GAAGGATCAGCTCAATTTGCTGCTCAGATATTTGGGTTAAGCAACCATTTGGTATGGAGC; AAACTGCGAGCAAGCATTTTGAACACATGGATTTCCTTGAAGCAGGCTGACAAGAAAATC; AGAGAATGTAATTTATAA; ; Clone variation with respect to NM_006452.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006452 unedited; TTCAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTCTGC; CTCGCTTCCCGGCGCGGTCGCAGCCCTCAGCCCACTTAGGATAATGGCGACAGCTGAGGT; ACTGAACATTGGTAAAAAATTATATGAGGGTAAAACAAAAGAAGTCTACGAATTGTTAGA; CAGTCCAGGAAAAGTCCTCCTGCAGTCCAAGGACCAGATTACAGCAGGAAATGCAGCTAG; AAAAAACCACCTGGAAGGAAAAGCTGCAATCTCAAATAAAATCACCAGTTGTATTTTTCA; GTTATTACAGGAAGCAGGTATTAAAACTGCCTTCACCAGAAAATGTGGGGAGACAGCTTT; CATTGCACCGCAGTGTGAAATGATTCCAATTGAATGGGTTTGCAGAAGAATAGCAACTGG; TTCTTTTCTCAAAAGAAATCCTGGTGTCAAGGAAGGATATAAGTTTTACCCACCTAAAGT; GGAGTTGTTTTTCAAGGATGATGCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGAT; TGCTGCAAAATTTTGCTTTGCTGGACTTCTTATAGGCCAGACTGAAGTGGATATCATGAG; TCATGCTACACAGGCTATATTTGAAATACTGGAGAAATCCTGGTTGCCCCAGAATTGTAC; ACTGGTTGATATGAAGATTGAATTTGGTGTNGATGNTACCACCAAAAGAATTNNGTCTGC; TGNATGTATTTGACATGATTCCTGGAGACTCTGGCCATCAGGAGATCGAAGCCCNACAGA; AGAACAACAGTCTTATCGGGACCTCAAAGAAGTACTCCCTGAGGGCNNTCAATGGNTAAG; NAAAAACTTGAGTGGGTTGCANAGAGAGTAGAGTTGCTTTTTGAATCAGAAGTCANTGCA; GGNTTGTANTGGTGAGGGCTCTACTTCTGATCTGGGTCACTGTGAAAAATCAGAAAGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006452 unedited; ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATTCACTAATACA; TTTTATTTGTGTTCTCTAATTTAAAATTACCTTTTCATCTTGCTTGATTTTCCTTCAGCT; AAATTAGAAATTTGTAGTTTTTCCCCTAAAAAATTCAATGGCATTCTTTCTTATAAATTA; CATTCTCTGATTTTCTTGTCAGCCTGCTTCAAGGAAATCCATGTGTTCAAAATGCTTGCT; CGCAGTTTGCTCCATACCAAATGGTTGCTTAACCCAAATATCTGAGCAGCAAATTGAGCT; GATCCTTCTGGAGAAAGTACGGTTGAACAGCCAAGACCACTGGGTAGTCGAAGAGAAGAC; CACACATCCTGAACTCCCCAGTCTGGTGTGAGGGGAGGACAGCTGATAACTGGATATGCA; GTGTTCCCAGACATCACTGGTCCCAAACCATTACTTCTGCCTGCCACTGCCACAAATACA; GTAGGAATGCCATCCCCTTCATACTCAGCTTTAATCCTCAGAGTTTCATCTGGTCCTTTA; TGCGCAGATGTTACTCGAAGTTCACATGGAATGCCAAAATTTCCACAGGCCTTCTTGATT; TTTTCACAGTGACCAAGATCAGAAGTAGAGCCCATCAACACTACAACCCTGCACTGACTT; TCTGATTTCAAAAGCAACTCTACTCTCTCTGCACCCACTCAAAGTTTTTCTTTACCATTT; GGAGCCCTTCANGAGTTACTTCTTTGAGGTCCCGNATAGACTGTTTGTCTTTCTGTGGCC; TTCGATCTCCTGATGGCCANAGTCTCCAGAAATCATTGTCAATAACATCAGCCAGAACAA; TTTCTTTGGGTGGTACATCACACCAAATTCAATCTTCATATCAACCAGTGTACAATTCTG; GGGCAACCAGGATTCTCCAGTATTNACAAATAGCCTGGTAACATGACTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006452
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006452.2, NP_006443.1
• RefSeq Size: 3322 bp
• RefSeq ORF: 1278 bp
• Locus ID: 10606
• UniProt ID: P22234
• Cytogenetics: 4q12
• Domains: AIRC, SAICAR_synt
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Summary: This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the CDS, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Variants 2 and 3 encode the same isoform.