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UBA1 (NM_003334) Human Untagged Clone

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SKU
SC111049
UBA1 (untagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1
  $922.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UBA1
Synonyms A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111049 sequence for NM_003334 edited (data generated by NextGen Sequencing)
ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGT
TCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGA
ATGGCCAAGAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTAT
GTGTTGGGCCATGAGGCAATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTG
CGGGGCCTGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACC
CTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTACCTGCGGGAG
GAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGC
TATGTGCCTGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAG
GTGGTGGTGCTCACCAACACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCAC
AACCGTGGCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTCTGT
GACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATG
GTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCTGCCTGGATGAGGCCCGACAC
GGGTTTGAGAGCGGGGACTTTGTCTCCTTTTCAGAAGTACAGGGCATGGTTGAACTCAAC
GGAAATCAGCCCATGGAGATCAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACC
TCCAACTTCTCCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAG
ATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGTGACGGACTTC
GCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCTTCCAGGCCCTGCACCAGTTCTGT
GCTCAGCATGGCCGGCCACCTCGGCCCCGCAATGAGGAGGATGCAGCAGAACTGGTAGCC
TTAGCACAGGCTGTGAATGCTCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAG
GACCTCATCCGGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCCTTC
ATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTTCATGCCCATC
ATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTGAGGACAAAGAGGTCCTCACA
GAGGACAAGTGCCTCCAGCGCCAGAACCGTTATGACGGGCAAGTGGCTGTGTTTGGCTCA
GACCTGCAAGAGAAGCTGGGCAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGC
TGTGAGCTGCTCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAAATC
ATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACAGTTTCTTTTCCGG
CCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTGCAGCTGTGCGCCAAATGAAT
CCACATATCCGGGTGACAAGCCACCAGAACCGTGTGGGTCCTGACACGGAGCGCATCTAT
GATGACGATTTTTTCCAAAACCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCC
CGCATGTACATGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACA
CTGGGCACCAAAGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGAGTCGTACAGTTCC
AGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTACCCTGAAGAACTTCCCTAATGCC
ATCGAGCACACCCTGCAGTGGGCTCGGGATGAGTTTGAAGGCCTCTTCAAGCAGCCAGCA
GAAAATGTCAACCAGTACCTCACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCA
GGCACTCAGCCCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTCGAAC
AACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACCAGCTCACAAGCTCAGGAGCGCCG
TTCTGGTCTGGGCCCAAACGCTGTCCACACCCGCTCACCTTTGATGTCAACAATCCCCTG
CATCTGGACTATGTGATGGCTGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGC
TCTCAGGACCGAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTCACC
CCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGCCAATGCCTCT
GTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGCCCAGCCCAGACAAGCTCCCT
GGATTCAAGATGTACCCCATTGACTTTGAGAAGGATGATGACAGCAACTTTCATATGGAT
TTCATCGTGGCTGCATCCAACCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGG
CACAAGAGCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCC
GTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCACCGACAGCTTGAC
TCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCTTTGGTTTCTCTGAACCCCTT
GCCGCACCACGTCACCAGTACTATAACCAAGAGTGGACATTGTGGGATCGCTTTGAGGTA
CAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAG
ACAGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTC
TTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGC
CGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGGCGCTGGTGCTTGAGCTGTGCTGT
AACGACGAGAGCGGCGAGGATGTCGAGGTTCCCTATGTCCGATACACCATCCGCTGA

Clone variation with respect to NM_003334.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003334 unedited
GGGTTCAAATTTATGTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGGCTT
GGCTTCGGCTCCTTGAGGAGTTGGCGGCGGCGCGACCCGGGGAACCGGCATTGATGTCCA
GCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACT
GCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCA
AGAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGG
GCCATGAGGCAATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCC
TGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACCCTACATG
ACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTACCTGCGGGAGGAGGACA
TCGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGCTATGTGC
CTGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGG
TGCTCACCAACACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTG
GCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTCTGTGACTTTG
GAGAGGAAATGATCCTCACAGATTTCAATGGNGAGCAGCCACTCAGTGCTATGGTTTCTA
TGGTTACCAAGGACACCCCCGTGTGGTTACCTGCCTGGATGANGCCCGACACCGGTTTGA
GAGCGGGGACTTTGTCTCCTTTTAANAATACACGGCATGGTTGAACCTAACGGAAATCAG
N
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003334 unedited
GGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTGTGGGAGTTATTAATTCTTTAT
TAGGATTCAGGTTTCAAACAAGGTAGAAGGCAGTGGCAAGAGGGGTTCAGGTAGGGGGAT
GATGAGGGAACACCAGACTTGGCTAGTTGGGCCACTGCCAGAAGCCAAATGGGAACCCTG
GGCTGGAAGGGGTGTGGAGAGGGGTGGACAAGGGGCCAGCCTAAAGGAGCAGACGGGGTC
AGCGGATGGTGTATCGGACATAGGGAACCTCGACATCCTCGCCGCTCTCGTCGTTACAGC
ACAGCTCAAGCACCAGCGCCCGCACGTGGCGGCCCAGCTTTCGCTTCGACACACGGCTCA
CAATCTCTGTCATCGGCTGATCCAACCGTTCCTTGAGCTTGGCAGCTGGCATGAAGAAGG
AATAGAGCATGGACACGCCCTGGGACAGCATGGTGATCTCTAATTTGTGCTCTGTCTTAA
AATAGTCGAGGAACTGTTTGAGGGTCATCTCCTCACCATTAGGCTGCAGCCCTTGTACCT
CAAAGCGATCCCACAATGTCCACTCTTGGTTATAGTACTGGTGACGTGGTGCGGCAAGGG
GTTCAGAGAAACCAAAGAAAGGCAGGGCCAAGTTGAGGAAACCATTCTTGTANGAGTCAA
GCTGTCGGTGCCCCTGCACAACCTTGTACAGCTCCAGACACACAAGGCCAACCACGGNTT
GCTGTGGTCGTGGCAATGGCTTGGGATGATCTTCCCCTGAATCAACTTGCTCTTGTGCCC
GGGCTGCACAAAGAAAGTCATTATTTTCTGCCCCGAAGTTGGATGCCCCCCCNATGAAAT
CCCTATGAAAGGTTGTTGCATTATCCCTCCTCAAGACAATGGGGTCACTTTTGAATCCAG
GGACCTTGTTTGGGTCTGGCAAAATAGGTTTTTAACTCTCTTATACAATGCCTCAAAAGA
GGCATTGCGCCTCTCCACTTCCGG
Restriction Sites NotI-NotI |
ACCN NM_003334
Insert Size 3520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003334.2, NP_003325.2
RefSeq Size 3504 bp
RefSeq ORF 3177 bp
Locus ID 7317
UniProt ID P22314
Cytogenetics Xp11.3
Domains ThiF, UBACT
Protein Pathways Parkinson's disease, Ubiquitin mediated proteolysis
Summary The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_003334" in other vectors (7)
SKU Description Size Price
RC201446 UBA1 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1 10 ug
$921.00
RC201446L1 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,221.00
RC201446L2 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1, mGFP tagged 10 ug
$1,221.00
RC201446L3 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,221.00
RC201446L4 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1, mGFP tagged 10 ug
$1,221.00
RG201446 UBA1 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1 10 ug
$789.00 $1,121.00
SC320329 UBA1 (untagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 1 10 ug
$922.00

file-alt Citations

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