TRAF2 (NM_021138) Human Untagged Clone

SKU SC111044

TRAF2 (untagged)-Human TNF receptor-associated factor 2 (TRAF2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRAF2
• Synonyms: MGC:45012; RNF117; TRAP; TRAP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021138 edited; GAATTCGGCACGAGGGAACCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCCTCAGGTGT; GCTACGTTCCCGCTCTCGGGGCTGGAGGGGAACCCGTGGTAGCTATAGGGAGTGCACTAG; CCAGCCTCAGATGTGCTACGTTCCCGCTCTCAGGGCTGGAGGGGAACCCGTGGTAGCTAA; AGGGAGTGCACTAGCCAGCTCCAGGTGTGCTACATTCCCGCTCTCGGGGCTGGAGGGGAA; CCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCCCCAGGTGTGCTACATTCCCGCTCTAG; GGGCTGGTCTGGCGGCATGGCTCTCTCCTGAGTGCCTTCAGAGCCTGAGTGCCAGCAGGC; AGGCGTCTCCTGCCCAGTGGGTTTGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTG; CAGCTAGCGTGACCCCCCCTGGCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCC; TCCTGGGGACCAAGCTGGAAGCCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGCA; GGCCCTTCCAGGCGCAGTGTGGCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCA; GCTCTGGGCCTCAGAACTGTGCTGCCTGTGTTCACGAGGGCATATATGAAGAAGGCATTT; CTATTTTAGAAAGCAGTTCGGCCTTCCCAGATAATGCTGCCCGCAGGGAGGTGGAGAGCC; TGCCGGCCGTCTGTCCCAGTGATGGATGCACCTGGAAGGGGACCCTGAAAGAATACGAGA; GCTGCCACGAAGGCCGCTGCCCGCTCATGCTGACCGAATGTCCCGCGTGCAAAGGCCTGG; TCCGCCTTGGTGAAAAGGAGCGCCACCTGGAGCACGAGTGCCCGGAGAGAAGCCTGAGCT; GCCGGCATTGCCGGGCACCCTGCTGCGGAGCAGACGTGAAGGCGCACCACGAGGTCTGCC; CCAAGTTCCCCTTAACTTGTGACGGCTGCGGCAAGAAGAAGATCCCCCGGGAGAAGTTTC; AGGACCACGTCAAGACTTGTGGCAAGTGTCGAGTCCCTTGCAGATTCCACGCCATCGGCT; GCCTCGAGACGGTAGAGGGTGAGAAACAGCAGGAGCACGAGGTGCAGTGGCTGCGGGAGC; ACCTGGCCATGCTACTGAGCTCGGTGCTGGAGGCAAAGCCCCTCTTGGGAGACCAGAGCC; ACGCGGGGTCAGAGCTCCTGCAGAGGTGCGAGAGCCTGGAGAAGAAGACGGCCACTTTTG; AGAACATTGTCTGCGTCCTGAACCGGGAGGTGGAGAGGGTGGCCATGACTGCCGAGGCCT; GCAGCCGGCAGCACCGGCTGGACCAAGACAAGATTGAAGCCCTGAGTAGCAAGGTGCAGC; AGCTGGAGAGGAGCATTGGCCTCAAGGACCTGGCGATGGCTGACTTGGAGCAGAAGGTCT; TGGAGATGGAGGCATCCACCTACGATGGGGTCTTCATCTGGAAGATCTCAGACTTCGCCA; GGAAGCGCCAGGAAGCTGTGGCTGGCCGCATACCCGCCATCTTCTCCCCAGCCTTCTACA; CCAGCAGGTACGGCTACAAGATGTGTCTGCGTATCTACCTGAACGGCGACGGCACCGGGC; GAGGAACACACCTGTCCCTCTTCTTTGTGGTGATGAAGGGCCCGAATGACGCCCTGCTGC; GGTGGCCCTTCAACCAGAAGGTGACCTTAATGCTGCTCGACCAGAATAACCGGGAGCACG; TGATTGACGCCTTCAGGCCCGACGTGACTTCATCCTCTTTTCAGAGGCCAGTCAACGACA; TGAACATCGCAAGCGGCTGCCCCCTCTTCTGCCCCGTCTCCAAGATGGAGGCAAAGAATT; CCTACGTGCGGGACGATGCCATCTTCATCAAGGCCATTGTGGACCTGACAGGGCTCTAAC; TGCCCCCTACTGGTGTCTGGGGGTTGGGGGCAGCCAGGCACAGCCGGCTCACGGAGGGGC; CACCACACTGGGCCAGGGTCTCACTGTACAAGTGGGCAGGGGCCGCGCTTGGGCGCTTGG; GAGGGTGTCGGCCTGCAGCCAAGTTCACTGTCACGGGGGAAGGAGCCACCAGCCAGTCCT; CAGATTTCAGAGACTGCGGAGGGGCTTGGCAGACGGTCTTAGCCAAGGGCTGTGGTGGCA; TTGGCCGAGGGTCTTCGGGTGCTTCCCAGCACAAGCTGCCCTTGCTGTCCTGTGCAGTGA; AGGGAGAGGCCCTGGGTGGGGGACACTCAGAGTGGGAGCACATCCCAGCAGTGCCCATGT; AGCAGGAGCACAGTGGATGGCCTTGTGTCCCTCGGGCATGACAGGCAGAAACGAGGGCTG; CTCCAGGAGAAGGGCCTCCTGCTGGCCAGAGCAAGGAAGGCTGAGCAGCTTGGTTCTCCC; CTCTGGCCCCTGGAGAGAAGGGGGAGCATTCCTAGACCCCTGGGTGCTTGTCTGCACAGA; GCTCTGGTCTGTGCCACCTTGGCCAGGCTGGCTGTGGGAGGGTCTGGTCCCACGCCGCCT; CTGCTCAGACCACTGTGTGGGAGGTGCACAGCACAGCCTGCGGGTAAAGTGTGAGAGCTT; GCCATCCAGCTCACGAAGACAGAGTTATTAAACCATTCAAATCTCAAAAAAAAAAAAAAA; AAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021138 unedited; TACATAATTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGAGGAACCC; GTGGTAGCTAAAGGGAGTGCACTAGCCAGCCTCAGGTGTGCTACGTTCCCGCTCTCGGGG; CTGGAGGGGAACCCGTGGTAGCTATAGGGAGTGCACTAGCCAGCCTCAGATGTGCTACGT; TCCCGCTCTCAGGGCTGGAGGGGAACCCGTGGTAGCTAAAGGGAGTGCACTAGCCAGCTC; CAGGTGTGCTACATTCCCGCTCTCGGGGCTGGAGGGGAACCCGTGGTAGCTAAAGGGAGT; GCACTAGCCAGCCCCAGGTGTGCTACATTCCCGCTCTAGGGGCTGGTCTGGCGGCATGGC; TCTCTCCTGAGTGCCTTCAGAGCCTGAGTGCCAGCAGGCAGGCGTCTCCTGCCCAGTGGG; TTTGGGCTTTGTTCGCGGGGGTCACAGCTCTCATGGCTGCAGCTAGCGTGACCCCCCCTG; GCTCCCTGGAGTTGCTACAGCCCGGCTTCTCCAAGACCCTCCTGGGGACCAAGCTGGAAG; CCAAGTACCTGTGCTCCGCCTGCAGAAACGTCCTCCGCAGGCCCTTCCAGGCGCAGTGTG; GCCACCGGTACTGCTCCTTCTGCCTGGCCAGCATCCTCAGCTCTGGGCCTCAGAACTGTG; CTGCCTGTGTTCACGATGGCATATATGAAGAAGGCATTTCTATTTTAGAAAGCAGTTCGG; CCTTCCCAGATAATGCTGCCCGCANGGAGGTGGAGAGCCTGCNCGCCGTCTGTCCCAGTG; ATGGATGCACCTGGAAGGNGACCCCTGAAGAATACGAGAGCTGCCACGAAAGCCGCTGNC; CGCTCATGCTGACCGAAATGTCCCGCGTGNCAAGGCCTTGGTCGCCTGGNTGAAAATGAG; CGCACCTGGAGCACGGTGCCCCGANAGATCCTGAGCTGCCNGCATTGCGGNCACCTGCTG; CGGACANAGTGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021138 unedited; TTTTTTTTTTTTTTTTTNGAAATTTGATGGTTTAATAACTCTGTCTTCGTGAGCTGGATG; GCAAGCTCTCACACTTTACCCGCAGGCTGTGCTGTGCACCTCCCACACAGTGGTCTGAGC; AAAGGCGGCGTGGGACCAGACCCTCCCACAGCCAGCCTGGCCAAGGTGGCACAGACCAGA; GCTCTGTGCAGACAAGCACCCAGGGGTCTAGGAATGCTCCCCCTTCTCTCCAGGGGCCAG; AGGGGAGAACCAAGCTGCTCAGCCTTCCTTGCTCTGGCCAGCAGGAGGCCCTTCTCCTGG; AGCAGCCCTCGTTTCTGCCTGTCATGCCCGAGGGACACAAGGCCATCCACTGTGCTCCTG; CTACATGGGCACTGCTGGGATGTGCTCCCACTCTGAGTGTCCCCCACCCAGGGCCTCTCC; CTTCACTGCACAGGACAGCAAGGGCAGCTTGTGCTGGGAAGCACCCGAAGACCCTCGGCC; AATGCCACCACAGCCCTTGGCTAAGACCGTCTGCCAAGCCCCTCCGCAGTCTCTGAAATC; TGAGGACTGGCTGGTGGCTCCTTCCCCCGTGACAGTGAACTTGGCTGCAGGCCGACACCC; TCCCAAGCGCCCAAGCGCGGCCCCTGCCCACTTGTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_021138
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021138.3, NP_066961.2
• RefSeq Size: 2298 bp
• RefSeq ORF: 1506 bp
• Locus ID: 7186
• UniProt ID: Q12933
• Cytogenetics: 9q34.3
• Domains: MATH, RING, zf-TRAF
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, MAPK signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer
• Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes a longer isoform (1).