SEC13L1 (SEC13) (NM_183352) Human Untagged Clone

SKU SC111014

SEC13 (untagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SEC13L1
• Synonyms: D3S1231E; npp-20; SEC13L1; SEC13R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111014 sequence for NM_183352 edited (data generated by NextGen Sequencing); ATGGTGTCAGTAATTAACACTGTGGATACCTCCCATGAGGACATGATTCACGACGCCCAG; ATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCAAAATCTTT; GATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTG; TGGCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGAC; CGGAAAGTCATTATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCG; GGACACGACTCCTCAGTGAACTCGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTG; GCCTGTGGGAGCTCGGATGGGGCCATCTCCCTGCTGACTTACACCGGGGAAGGCCAATGG; GAAGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCT; GCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATTACATCAAG; AGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGC; CAGTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGG; GCCCCCTCCATCGGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTG; TTCATTTGGACCTGTGATGATGCCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAG; TTCAACGATGTGGTGTGGCATGTGAGCTGGTCCATCACAGCCAACATCCTGGCTGTCTCT; GGTGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGATGGGCAGTGGGTGTGCATC; AGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCCAGCAGAAC; GAGCAGTGA; ; Clone variation with respect to NM_183352.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183352 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGNCCGGGAGCTGC; CACGTCCGAGACCTGGAGCAGCCACCGCCGCAATCATGGTGTCAGTAATTAACACTGTGG; ATACCTCCCATGAGGACATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGG; CAACCTGCTCATCAGACAGGTCCGTCAAAATCTTTGATGTGCGCAATGGAGGGCAGATCC; TTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTGGCAAGTGGCCTGGGCTCACCCCA; TGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATTATCTGGAGAGAGG; AAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACTCGG; TGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCA; TCTCCCTGCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTC; ACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAG; ACCACCCATCGGGGCAGAAACCCAATTACATCAAGAGGTTTGCATCAGGTGGCTGTGACA; ACCTCATCAAGCTGTGGAAGGAGGAGGANGACGGCCAGTGGAAGGAGGAGCAGAAGCTAG; AAGCGCACAGTGACTGGNGTCGAGATGTGGGCTGGNGCCCCTNCATCGGNCTGCCCACCA; GCACCATCGNCAGCTGCTCCAGGATGGTCGTGTTTCATTTTGACCTGTGATGATGCCTAA; GCATACGTGGTCCCTAAANTGTGCACAAGTCAACGATGTGTGTTGCATGTGAGCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_183352 unedited; GCCGCATTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTATAAGCAATATGACTT; TATTTAGTTCCTTTGGAAACAAAACCCCCCAAAAATGCCTGAACCCAAAGGTACATAAAA; ATGACCCAAAATAGATTTGAACATCACTTGTAGTTTCTTCCTCGTAACATGAGTGCTTTC; AGCTGGACAGTAGATTACAAAGCATCTCCGATCACGTTAAGGCAGATGATCAATCTGTGG; CTGCATCTGTAACTCCTCCTGGGAAAATAATCCTGTTGGAGTTGGGGGCTCTTCCCAGTT; GTTTGGTTAGTTGGCCCAGGAAGGGGCAGTCCTGGAGCTGGCGGGTGGGGAGCCAGGCCC; CACCTGTCTTGTCACTGCTCGTTCTGCTGGCCCTCTGTCACTGATGCTGATACGGAGCCC; TGGCCCTTGTTGACATCACTGATGCACACCCACTGCCCATCAACTGACTCCTTCCACAGG; GTCACCTTATTGTCTCCACCAGAGACAGCCAGGATGTTGGCTGTGATGGACCANCTCACA; TGCCACACCACATCGTTGAACTTGTGCAACAATTTAGGGGACCACGTATTGCTTGAGGCA; TCATCACAGGTCCAAATGAACACACGACCATCCTGGGAGCANCTGGCGATGGTGCTGGTG; GGCAGGCCGATGGAGGGGGCCCAGGCCACATCTCGAACCCAGTCACTGTGCGCTTCTAGC; TTCTGCTCCTCCTTACACTGGCCGGGCTCCTCCTTCTTCCACAGTTTGATGAAGGTTGTC; ACAGCCCCTGATGCCAACCTTTGAAGTAATCGGTCTCTGCCCCGATGGCTGGTCTATGAG; CCTTCAAGTACACAGCAAGGGCCCACCTGACGCATTGCACCAATGTGTAGCGTTGTGATC; TTTTTACTTCCATTGGCTTTCCCGGGTAGTCACAAGGAGAGGCCCCATCCACTCCCCAGG; CAGTATAGGCGTATTCTGTGGGCCCCCCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_183352
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_183352.1, NP_899195.1
• RefSeq Size: 1428 bp
• RefSeq ORF: 969 bp
• Locus ID: 6396
• UniProt ID: P55735
• Cytogenetics: 3p25.3
• Summary: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]; Transcript Variant: This variant (1) differs in the 5' UTR and lacks an exon in the 5' coding region, compared to variant 3. It encodes isoform 1 which has a shorter and distinct N-terminus, compared to isoform 3.