ORC2 (NM_006190) Human Untagged Clone

SKU SC110965

ORC2 (untagged)-Human origin recognition complex, subunit 2 (ORC2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ORC2
• Synonyms: ORC2L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110965 sequence for NM_006190 edited (data generated by NextGen Sequencing); ATGAGTAAACCAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGAT; GATGTTCTTAATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCG; CAGCTTTTGGTCAACCCCAAAAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGAT; GACCAGGAGGTCTTAAAAGATCAGAACTATGTGGAAATTATGGGAAGAGATGTTCAAGAA; TCATTGAAAAATGGCTCTGCTACAGGTGGTGGAAATAAAGTTTATTCTTTTCAGAATAGA; AAACACTCTGAAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACCACAAAAAAGT; GTTTCATTCAGTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCAAAGTAGCAAG; GGCCATTCTGCTTCAGACAAGGTTCAACCGAAGAACAATGACAAAAGTGAATTTCTGTCA; ACAGCACCTCGTAGTCTAAGAAAAAGATTAATAGTTCCAAGGTCTCATTCTGACAGTGAA; AGCGAATATTCTGCTTCCAACTCAGAGGATGATGAAGGGGTTGCACAGGAACATGAAGAG; GACACTAATGCAGTCATATTCAGCCAAAAGATTCAAGCTCAGAATAGAGTAGTTTCAGCT; CCTGTTGGCAAAGAAACACCTTCTAAGAGAATGAAAAGAGATAAAACAAGTGACTTAGTA; GAAGAATATTTTGAAGCTCACAGCAGTTCAAAAGTTTTAACCTCTGATAGAACACTGCAG; AAGCTAAAGAGAGCTAAACTGGATCAGCAAACTTTGCGTAACTTATTGAGCAAGGTTTCC; CCTTCCTTTTCTGCCGAACTTAAACAACTAAATCAACAGTATGAAAAATTATTTCATAAA; TGGATGCTGCAATTACACCTTGGGTTCAACATTGTGCTTTATGGTTTGGGTTCTAAGAGA; GATTTACTAGAAAGGTTTCGAACCACTATGCTGCAAGATTCCATTCACGTTGTCATCAAT; GGCTTCTTTCCTGGAATCAGTGTGAAATCAGTCCTGAATTCTATAACAGAAGAAGTCCTC; GATCATATGGGTACTTTCCGCAGTATACTGGATCAGCTAGACTGGATAGTAAACAAATTT; AAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAGCCAGATGTTG; AGAGGAGAGAAGAGCCAGCAAATCATTGGTCAGTTGTCATCTTTGCATAACATTTACCTT; ATAGCATCCATTGACCACCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTT; TTTAACTGGCTCTGGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTAT; GAGAACTCTCTTCTGGTAAAGCAGTCTGGATCCCTGCCACTTAGCTCCCTTACTCATGTC; TTACGAAGCCTTACCCCTAATGCAAGGGGAATTTTCAGGCTACTAATAAAATACCAGCTG; GACAACCAGGATAACCCTTCTTACATTGGCCTTTCTTTTCAAGATTTTTACCAGCAGTGT; CGGGAGGCATTCCTCGTCAATAGTGATCTGACACTCCGGGCCCAGTTAACTGAATTTAGG; GACCACAAGCTTATAAGAACAAAGAAGGGAACTGATGGAGTAGAGTATTTATTAATTCCT; GTTGATAATGGAACATTGACTGATTTCTTGGAAAAGGAAGAAGAGGAGGCTTGA; ; Clone variation with respect to NM_006190.4; 1173 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006190 unedited; AAACCGACTTTCCTATAGGCGGCCGCGCAATTCGCACGAGGGTCGGGCGCGTATTACTGG; AAATTGGCTTTTCCCGTTGGGGCCGAAGTACCTTCCCTGCGGCGGCGACTCAGCGGGGTG; TCGTTCGGCCGGCGTGACGCAGCCGGATCGGCGCCAGACGGAAACCTAGCGGTGACTGTA; TCTGAATTTTGCAGCTGCAGAATGTGTAGTACCTTAAAAGGTTGGCAACAATGAGTAAAC; CAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGATGATGTTCTTA; ATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCGCAGCTTTTGG; TCAACCCCAAAAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGATGACCAGGAGG; TCTTAAAAGATCAGAACTATGTGGAAATTATGGGAAGAGATGTTCAAGAATCATTGAAAA; ATGGCTCTGCTACAGGTGGTGGAAATAAAGTTTATTCTTTTCAGAATAGAAAACACTCTG; AAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACCACAAAAAAGTGTTTCATTCA; GTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCANAGTAGCAAGGGCCATTCTG; CTTNCAGACAGGTTCAACCGAAGAACCATGACAAAGTGAANTTCTGTCAACAGCACCTCG; TAGTCTAAGAAAAAGATTATAGTTNCCAGGTCTCATTCTGACAGTGAAAGCGAATATTCT; GCTTNCCCACTCAGAGATGATGAAGGGGCTGCACAGGAACCATGAGAGGGACACTATGCA; GTCATATTCAGCCAAAAGATTNCAGCTCAGAATANAGTAGTTNCAGCTCCTGGTGGCAAG; AAACACCTCTAAGAGAAATGAAGAGATAACAGTGACTTATAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006190 unedited; GTCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTGACGGAGAACGTATTTTATTGCAGGGATATTTTCAGGAAAAA; CTTCATTTCCAAGGGTCAAATATTTCAGGGGGTATGTGATGACTGTAAGCTGACAACAGT; AGCCAAGGGGAGCCTTTTTTTTTTCCTAAAAAAAAAGTCTCGCGCAAATATTCAGGAGGT; TTGAAATCATCACTTTAAGGAAAAAACTGAAATATCTTACAGGGAGTTTTAGGTCTACTT; GACAGAGGAATCAAAATTAATTTACAGCCCGCCATTAATATGCTACTCACCTGCATGTTG; TTCAAGAAAAGAAAAATTTGTCAATTTACAAGAATAATGAAAGGGTTTATTTTTTGGTGG; CTTGAAAACAATTACTAAAATGTAATTCAGGATTTTATTTCTTTAGCCTGGCTTTTCACT; GGGATCATGAAGGGCATGGCACAAACAATAACTGTTCCAAATATATGACGTATTGGTCCT; GGGCTTTCTAGGCTGGGTAGATACCCTATATACTCTGTTTTTCCCATTATCCCAACTGAA; TAGTATTATAATATATTCCCTCCCCTGCCCCCACCAAGTACATAGCCTCAATCCAAAAGG; AGAAAGCTGGATCACTCACCTCTTCCCAATCTACACCTTTTAAGTTGTTTTAGAAACAAT; AAGAGATGACCTGCCCTCTCCATTACACTCGTTGGGTTCTGACACACAGAATAGCAAACA; AAACCAATTTACGTGATGCCCATGTCTTTATACTTNCACACTCTGGAACAAACACACTTG; CAAACATCAGCTCGGGGAGTGTTAAAAGGCCCNTCAAAAATCAGAATATCTCTAGTTAAA; ATAATCAAAACATNCAGNTCCAATAGCCCTGAGCCACTGNTCATTCAGCAGAGTCTCAGA; GAGTATGAATGAAGGCCATTNTCTCACTGAGACCGTGCTATACTGACGTACAGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_006190
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006190.3, NP_006181.1
• RefSeq Size: 3138 bp
• RefSeq ORF: 1734 bp
• Locus ID: 4999
• UniProt ID: Q13416
• Cytogenetics: 2q33.1
• Domains: ORC2
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle
• Summary: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (1) encodes a functional protein.