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EGFR (NM_005228) Human Untagged Clone

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SKU
SC110878
EGFR (untagged)-Human epidermal growth factor receptor (EGFR), transcript variant 1
  $1,581.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EGFR
Synonyms ERBB; ERBB1; ERRP; HER1; mENA; NISBD2; PIG61
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_005228 edited
ATGCGACCCTCCGGGACGGCCGGGGCAGCGCTCCTGGCGCTGCTGGCTGCGCTCTGCCCG
GCGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAGCTCACGCAG
TTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATGTTCAATAACTGTGAGGTG
GTCCTTGGGAATTTGGAAATTACCTATGTGCAGAGGAATTATGATCTTTCCTTCTTAAAG
ACCATCCAGGAGGTGGCTGGTTATGTCCTCATTGCCCTCAACACAGTGGAGCGAATTCCT
TTGGAAAACCTGCAGATCATCAGAGGAAATATGTACTACGAAAATTCCTATGCCTTAGCA
GTCTTATCTAACTATGATGCAAATAAAACCGGACTGAAGGAGCTGCCCATGAGAAATTTA
CAGGAAATCCTGCATGGCGCCGTGCGGTTCAGCAACAACCCTGCCCTGTGCAACGTGGAG
AGCATCCAGTGGCGGGACATAGTCAGCAGTGACTTTCTCAGCAACATGTCGATGGACTTC
CAGAACCACCTGGGCAGCTGCCAAAAGTGTGATCCAAGCTGTCCCAATGGGAGCTGCTGG
GGTGCAGGAGAGGAGAACTGCCAGAAACTGACCAAAATCATCTGTGCCCAGCAGTGCTCC
GGGCGCTGCCGTGGCAAGTCCCCCAGTGACTGCTGCCACAACCAGTGTGCTGCAGGCTGC
ACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAATTCCGAGACGAAGCCACGTGC
AAGGACACCTGCCCCCCACTCATGCTCTACAACCCCACCACGTACCAGATGGATGTGAAC
CCCGAGGGCAAATACAGCTTTGGTGCCACCTGCGTGAAGAAGTGTCCCCGTAATTATGTG
GTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGCTATGAGATGGAGGAA
GACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGTGTGTAACGGAATA
GGTATTGGTGAATTTAAAGACTCACTCTCCATAAATGCTACGAATATTAAACACTTCAAA
AACTGCACCTCCATCAGTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCC
TTCACACATACTCCTCCTCTGGATCCACAGGAACTGGATATTCTGAAAACCGTAAAGGAA
ATCACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCTTT
GAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTCTTGCAGTC
GTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGATAAGTGATGGAGAT
GTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATACAATAAACTGGAAAAAACTG
TTTGGGACCTCCGGTCAGAAAACCAAAATTATAAGCAACAGAGGTGAAAACAGCTGCAAG
GCCACAGGCCAGGTCTGCCATGCCTTGTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCC
AAGGACTGCGTCTCTTGCCGGAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAAC
CTTCTGGAGGGTGAGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCA
GAGTGCCTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC
CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGAGTCATG
GGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGTGTGCCACCTGTGC
CATCCAAACTGCACCTACGGATGCACAGGGCCAGGTCTTGAAGGCTGTCCAACGAATGGG
CCTAAGATCCCGTCCATCGCCACTGGGATGGTGGGGGCCCTCCTCTTGCTGCTGGTGGTG
GCCCTGGGGATCGGCCTCTTCATGCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGG
AGGCTGCTGCAGGAGAGGGAGCTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAAC
CAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCC
GGTGCGTTCGGCACGGTGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATT
CCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTC
GATGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATC
TGCCTCACCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGAC
TATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAG
ATCGCAAAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCC
AGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAA
CTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAGTGCCTATCAAGTGG
ATGGCATTGGAATCAATTTTACACAGAATCTATACCCACCAGAGTGATGTCTGGAGCTAC
GGGGTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCCCTGCC
AGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATATGTACC
ATCGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGACGCAGATAGTCGCCCAAAG
TTCCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCTACCTTGTC
ATTCAGGGGGATGAAAGAATGCATTTGCCAAGTCCTACAGACTCCAACTTCTACCGTGCC
CTGATGGATGAAGAAGACATGGACGACGTGGTGGATGCCGACGAGTACCTCATCCCACAG
CAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGCTCTCTGAGTGCA
ACCAGCAACAATTCCACCGTGGCTTGCATTGATAGAAATGGGCTGCAAAGCTGTCCCATC
AAGGAAGACAGCTTCTTGCAGCGATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGAC
AGCATAGACGACACCTTCCTCCCAGTGCCTGAATACATAAACCAGTCCGTTCCCAAAAGG
CCCGCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGCCTCTGAACCCCGCGCCCAGC
AGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTGGGCAACCCCGAGTATCTCAAC
ACTGTCCAGCCCACCTGTGTCAACAGCACATTCGACAGCCCTGCCCACTGGGCCCAGAAA
GGCAGCCACCAAATTAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAA
GCCAAGCCAAATGGCATCTTTAAGGGCTCCACAGCTGAAAATGCAGAATACCTAAGGGTC
GCGCCACAAAGCAGTGAATTTATTGGAGCATGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005228 unedited
GCGCCCGCCGCGGCCGAGGCGGCCGGAGTCCCGAGCTAGCCCCGGCGGCCGCCGCCGCCC
AGACCGGACGACAGGCCACCTCGTCGGCGTCCGCCCGAGTCCCCGCCTCGCCGCCAACGC
CACAACCACCGCGCACGGCCCCCTGACTCCGTCCAGTATTGATCGGGAGAGCCGGAGCGA
GCTCTTCGGGGAGCAGCGATGCGACCCTCCGGGACGGCCGGGGCAGCGCTCCTGGCGCTG
CTGGCTGCGCTCTGCCCGGCGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACG
AGTAACAAGCTCACGCAGTTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATG
TTCAATAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTATGTGCAGAGGAATTAT
GATCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGTTATGTCCTCATTGCCCTCAAC
ACAGTGGAGCGAATTCCTTTGGAAAACCTGCAGATCATCAGAGGAAATATGTACTACGAA
AATTCCTATGCCTTAGCAGTCTTATCTAACTATGATGCAAATAAAACCGGACTGANAGAG
CTGCCCATGAGAAATNTACAGGAAATCCTGCATGGCGCCGTGCGGGTCAGCAACANCCCT
GCCCTGTGNCACGTGGAGAGCATNCCTTGGCGGGACATAGTCAGCAGTGACTTTCTAGCC
ACATGTCGATGGACTTTCAGAACCACTGGGCAGCTGCCAAAGTGTGATCCAAGCTGTCCC
ATGGGAGCTGCTGGGGTGCAAGAGAAGAAACTGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005228 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAATATACCTCTTTGAAAG
ATAAATTTCTGCTCAAAGGACAATATTCTTGCTGGATGCGTTTCTGTAAATGCTTCACAG
TTTGAAGACAAAGGAATGCAACTTCCCAAAATGTGCCCGAGGTGGAAGTACTTCCTGGCT
AGTCGGTGTAAACGTTGCAAAACCAGTCTGTGGGTCTAAGAGCTAATGCGGGCATGGCTG
TTGGGATGGAGGACCTGCTGTGGCTTGGTCCTGGGTATCGAAAGAGTCTGGATTTTTAGG
GCTCATACTATCCTCCGTGGTCATGCTCCATTAAATCCACTGCTTTGCGGCGCGACCCTT
AAGTATCCTGCTTCTTCAGCTGGGGAGCCTTTAAAGATGCCTTTTCGCTCGCCTCCCTCG
CAAAAAACATCTTGTCTGCACCCAGCCTCCCCCACGTTACTTGCCGGCTCTCCTTCTGCC
CCCCCTTACCGCCCTCTCTTCCCCCCTTTCTTTTTTTCTGACTGCATCTTCCTCGTATTT
TCCCTTTCCATTTTTCCCTTTCCTCTCCTCCCCTTCCCTCTTCCTTTCTTTCTTTTCTCC
CCTCTCTCCCACTCTGTCCTTTGACTCATCCTTCTCCCTTTTCTTTCCTATTTCCCCCCC
CCCTCATTTTTTATCCCATCTCCCCCCTCCTTCTCCTCCCCCCCTTTTTTTTCTCTTCTC
CTCTCTCCTTTCACTTTTCATTTTTTTCCTCCCCCTTATTCCTCTTTCTTCCCCACCCTT
TCCTTTTCTTTCATATCCCTCCCCTCGCACCCCTTCCTTCTTTCCTCCACCCCTCCTCTC
CTTATTTCCTTTTTTTCCTTCCTCCCTCTTTCTTGTTCCTCTATTTCTCTCTCCCCCCGC
TCTTCCTCCCTCGAATCCTATCCTTTCTCTTTCATTTCCCTATTACTTNATCTCTTTCCC
TCCCCTTTCCCTCCTCCCCATTATTCTCTCCCCCTTNTTTTTTTTATTTCCCTCCCCCA
Restriction Sites NotI-NotI |
ACCN NM_005228
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005228.3, NP_005219.2
RefSeq Size 5616 bp
RefSeq ORF 3633 bp
Locus ID 1956
UniProt ID P00533
Cytogenetics 7p11.2
Domains FU, Furin-like, pkinase, Recep_L_domain, S_TKc, TyrKc
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton
Summary The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). provided by RefSeq, Jul 2020
Transcript Variant: This variant (1) encodes the longest isoform (a).
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Documents
Resources
"NM_005228" in other vectors (7)
SKU Description Size Price
RC217384 EGFR (Myc-DDK-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 1 10 ug
$589.00 $1,580.00
RC217384L1 Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,880.00
RC217384L2 Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 1, mGFP tagged 10 ug
$1,880.00
RC217384L3 Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,880.00
RC217384L4 Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 1, mGFP tagged 10 ug
$1,880.00
RC600001 EGFR (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens epidermal growth factor receptor, transcript variant 1, Signal peptide (1-24) plus EC domain (25-645) 10 ug
$289.00 $600.00
RG217384 EGFR (tGFP-tagged) - Human epidermal growth factor receptor (EGFR), transcript variant 1 10 ug
$789.00 $1,780.00

file-alt Citations

PDGF induced microRNA alterations in cancer cells
Shao, Rossi, Chelladurai et al
Nucleic Acids Res. (2011) 39 (10), 4035-47 DOI: 10.1093/nar/gkq1305
Cited by 47
AI Summary: OriGene’s pMirTarget-EGFR 3′-UTR reporter clone (SC216236) was used in this study to assess how PDGF-induced microRNAs regulate EGFR expression in cancer cells.
Snippet: Typically miR or gene knock-down or overexpression were achieved within 24–48 h of transfection. ... A Dual-Luciferase Assay (Promega) was performed to quantify EGFR 3′-UTR activities. EGFR 3′-UTR reporter clone pMirTarget-EGFR was from Origene (SC216236).
FigureFigureFigure
Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Gaborit, Larbouret, Vallaghe et al
J. Biol. Chem. (2011) 286 (13), 11337-45 DOI: 10.1074/jbc.M111.223503
Cited by 13
AI Summary: OriGene products pCMV6-XL4-EGFR and pCMV6-XL4-ERBB2 plasmids were used as sources for EGFR and HER2 transcripts, enabling the study of EGFR/HER2 dimerization and its disruption by targeted therapies in this research.
Snippet: 2) and HER2 transcript variant 1 (NM_004448.2) were isolated from pCMV6-XL4-EGFR and pCMV6-XL4-ERBB2 (Origene, CliniSciences, Montrouge ... 5A), and Lapatinib was more efficient than Pertuzumab to slow down tumor progression (p <0.001) as shown by the curve
Showing 1-2 of 2 papers.
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