DUSP15 (NM_177991) Human Untagged Clone

SKU
SC110786
DUSP15 (untagged)-Human dual specificity phosphatase 15 (DUSP15), transcript variant 2
$150.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DUSP15
Synonyms C20orf57; VHY
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC110786 sequence for NM_177991 edited (data generated by NextGen Sequencing)
ATGACTGTGACGGGGCTAGGCTGGCGGGACGTGCTTGAAGCCATCAAGGCCACCAGGCCC
ATCGCCAACCCCAACCCAGGCTTTAGGCAGCAGCTTGAAGAGTTTGGCTGGGCCAGTTCC
CAGAAGCTTCGCCGGCAGCTGGAGGAGCGCTTCGGCGAGAGCCCCTTCCGCGACGAGGAG
GAGTTGCGCGCGCTGCTGCCGCTGTGCAAGCGCTGCCGGCAGGGCTCCGCGACCTCGGCC
TCCTCCGCCGGGCCGCACTCAGCAGCCTCCGAGGGAACCGTGCAGCGCCTGGTGCCGCGC
ACGCCCCGGGAAGCCCACCGGCCGCTGCCGCTGCTGGCGCGCGTCAAGCAGACTTTCTCT
TGCCTCCCCCGGTGTCTGTCCCGCAAGGGCGGCAAGTGA

Clone variation with respect to NM_177991.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_177991 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGCCTCTGACCTT
CTGTTCTCGCCGGTCTCCGTGCGGCGGCATCTCCGTGTCCCTGTGACTCGAGACCGCGGC
TGTCGGGTCTCCCCCTCTCTGACCCTGCGTCTCTGTCCCCGCTGTGTCCGCCCTCTCTCC
GTGACTCTGTGTGTCTCTCGCTGTCTTGCTGTGGCTGTCACTCCCAGGCTGTCTCCCAGT
ACTTCCTGGACTCTACCTCGGAAACTTCATTGATGCCAAAGACCTGGATCAGCTGGGCCG
AAATAAGATCACACACATCATCTCTATCCATGAGTCACCCCAGCCTCTGCTGCAGGATAT
CACCTACCTTCGCATCCCGGTCGCTGATACCCCTGAGGTACCCATCAAAAAGCACTTCAA
AGAATGTATCAACTTCATCCACTGCTGCCGCCTTAATGGGGGGAACTGCCTTGTGCACTG
CTTTGCAGGCATCTCTCGCAGCACCACGATTGTGACAGCGTATGTGATGACTGTGACGGG
GCTAGGCTGGCGGGACGTGCTTGAAGCCATCAAGGCCACCAGGCCCATCGCCAACCCCAA
CCCAGGCTTTANGCAGCAGCTTGAAGAGTTTGGCTGGGCCAGTTCCCAGAAGCTTCGCCG
GCAGCTGGAGGAGCGCTTCGGCGAGAGCCCCTTCCGCGACGAGGGAGAGTTGCGCGCGCT
GCTGCCGCTGNGCAAGCGCTGCCGGCAGGGCTCCGCGACCTCGGCCTNCTNCGNCGGGCC
GNNACTAACAGCCTCCGAGGGACCGTGCNACGCCTGGTGCCCGCGCACGCCCCGGAAGCC
CACCGGCCGCTGCGCTGCTGGCGCGCCTNAANCANACTTTCTCTTGCCTCCCCGGGTCTG
CCCCGCAGCCGGCAGAGAGGAGCCC
Restriction Sites NotI-NotI
ACCN NM_177991
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177991.1, NP_817130.1
RefSeq Size 1184 bp
RefSeq ORF 399 bp
Locus ID 128853
UniProt ID Q9H1R2
Cytogenetics 20q11.21
Protein Families Druggable Genome, Phosphatase
Summary The protein encoded by this gene has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. This protein may function in the differentiation of oligodendrocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to isoform a. Variants 2 and 3 both encode isoform b, which has a shorter N-terminus than isoform a.
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Citations

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